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Genetic variation of promoter sequence modulates XBP1 expression and genetic risk for vitiligo.

Ren Y, Yang S, Xu S, Gao M, Huang W, Gao T, Fang Q, Quan C, Zhang C, Sun L, Liang Y, Han J, Wang Z, Zhang F, Zhou Y, Liu J, Zhang X - PLoS Genet. (2009)

Bottom Line: We then sequenced the variant in an additional 365 cases and 404 controls and found supporting evidence for the association (p_(trend) = 0.008, OR = 1.31, 95% CI = 1.07-1.59).The analysis of the combined 2,086 cases and 1,986 controls provided highly significant evidence for the association (p_(trend) = 2.94x10(-6), OR = 1.23, 95% CI = 1.13-1.35).Our subsequent functional study showed that the risk-associated C allele of rs2269577 had a stronger promoter activity than the non-risk G allele, and there was an elevated expression of XBP1 in the lesional skins of patients carrying the risk-associated C allele.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermatology and Department of Dermatology at No 1 Hospital, Anhui Medical University, Hefei, Anhui, China.

ABSTRACT
Our previous genome-wide linkage analysis identified a susceptibility locus for generalized vitiligo on 22q12. To search for susceptibility genes within the locus, we investigated a biological candidate gene, X-box binding protein 1(XBP1). First, we sequenced all the exons, exon-intron boundaries as well as some 5' and 3' flanking sequences of XBP1 in 319 cases and 294 controls of Chinese Hans. Of the 8 common variants identified, the significant association was observed at rs2269577 (p_(trend) = 0.007, OR = 1.36, 95% CI = 1.09-1.71), a putative regulatory polymorphism within the promoter region of XBP1. We then sequenced the variant in an additional 365 cases and 404 controls and found supporting evidence for the association (p_(trend) = 0.008, OR = 1.31, 95% CI = 1.07-1.59). To further validate the association, we genotyped the variant in another independent sample of 1,402 cases and 1,288 controls, including 94 parent-child trios, and confirmed the association by both case-control analysis (p_(trend) = 0.003, OR = 1.18, 95% CI = 1.06-1.32) and the family-based transmission disequilibrium test (TDT, p = 0.005, OR = 1.93, 95% CI = 1.21-3.07). The analysis of the combined 2,086 cases and 1,986 controls provided highly significant evidence for the association (p_(trend) = 2.94x10(-6), OR = 1.23, 95% CI = 1.13-1.35). Furthermore, we also found suggestive epistatic effect between rs2269577 and HLA-DRB1*07 allele on the development of vitiligo (p = 0.033). Our subsequent functional study showed that the risk-associated C allele of rs2269577 had a stronger promoter activity than the non-risk G allele, and there was an elevated expression of XBP1 in the lesional skins of patients carrying the risk-associated C allele. Therefore, our study has demonstrated that the transcriptional modulation of XBP1 expression by a germ-line regulatory polymorphism has an impact on the development of vitiligo.

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Related in: MedlinePlus

Immunohistochemical staining of XBP1 protein in the paired lesional (A,B) and non-lesional skin (C,D) of a patient with vitiligo (magnifications: (A,C)×200; (B,D)×400).
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pgen-1000523-g006: Immunohistochemical staining of XBP1 protein in the paired lesional (A,B) and non-lesional skin (C,D) of a patient with vitiligo (magnifications: (A,C)×200; (B,D)×400).

Mentions: We also performed immunohistochemistry analysis using formalin-fixed paraffin embedded skin biopsy samples from another 37 patients to assess the XBP1 protein level in the lesional and non-lesional skins. As shown in Figure 4 and Figure 5, there was a moderately enhanced XBP1 antibody staining in the lesional skin compared to the non-lesional skin (p = 0.003). Furthermore, the enhanced staining of XBP1 antibody in the lesional skin is more prominent in the patients carrying either CC (p = 0.04) or GC (p = 0.03) genotype. In the patients carrying the GG genotype, there was no significant enhancement (p = 0.41). An example of the enhanced XBP1 antibody staining in the lesional over the non-lesional skin of a patient is provided in Figure 6.


Genetic variation of promoter sequence modulates XBP1 expression and genetic risk for vitiligo.

Ren Y, Yang S, Xu S, Gao M, Huang W, Gao T, Fang Q, Quan C, Zhang C, Sun L, Liang Y, Han J, Wang Z, Zhang F, Zhou Y, Liu J, Zhang X - PLoS Genet. (2009)

Immunohistochemical staining of XBP1 protein in the paired lesional (A,B) and non-lesional skin (C,D) of a patient with vitiligo (magnifications: (A,C)×200; (B,D)×400).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2689933&req=5

pgen-1000523-g006: Immunohistochemical staining of XBP1 protein in the paired lesional (A,B) and non-lesional skin (C,D) of a patient with vitiligo (magnifications: (A,C)×200; (B,D)×400).
Mentions: We also performed immunohistochemistry analysis using formalin-fixed paraffin embedded skin biopsy samples from another 37 patients to assess the XBP1 protein level in the lesional and non-lesional skins. As shown in Figure 4 and Figure 5, there was a moderately enhanced XBP1 antibody staining in the lesional skin compared to the non-lesional skin (p = 0.003). Furthermore, the enhanced staining of XBP1 antibody in the lesional skin is more prominent in the patients carrying either CC (p = 0.04) or GC (p = 0.03) genotype. In the patients carrying the GG genotype, there was no significant enhancement (p = 0.41). An example of the enhanced XBP1 antibody staining in the lesional over the non-lesional skin of a patient is provided in Figure 6.

Bottom Line: We then sequenced the variant in an additional 365 cases and 404 controls and found supporting evidence for the association (p_(trend) = 0.008, OR = 1.31, 95% CI = 1.07-1.59).The analysis of the combined 2,086 cases and 1,986 controls provided highly significant evidence for the association (p_(trend) = 2.94x10(-6), OR = 1.23, 95% CI = 1.13-1.35).Our subsequent functional study showed that the risk-associated C allele of rs2269577 had a stronger promoter activity than the non-risk G allele, and there was an elevated expression of XBP1 in the lesional skins of patients carrying the risk-associated C allele.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermatology and Department of Dermatology at No 1 Hospital, Anhui Medical University, Hefei, Anhui, China.

ABSTRACT
Our previous genome-wide linkage analysis identified a susceptibility locus for generalized vitiligo on 22q12. To search for susceptibility genes within the locus, we investigated a biological candidate gene, X-box binding protein 1(XBP1). First, we sequenced all the exons, exon-intron boundaries as well as some 5' and 3' flanking sequences of XBP1 in 319 cases and 294 controls of Chinese Hans. Of the 8 common variants identified, the significant association was observed at rs2269577 (p_(trend) = 0.007, OR = 1.36, 95% CI = 1.09-1.71), a putative regulatory polymorphism within the promoter region of XBP1. We then sequenced the variant in an additional 365 cases and 404 controls and found supporting evidence for the association (p_(trend) = 0.008, OR = 1.31, 95% CI = 1.07-1.59). To further validate the association, we genotyped the variant in another independent sample of 1,402 cases and 1,288 controls, including 94 parent-child trios, and confirmed the association by both case-control analysis (p_(trend) = 0.003, OR = 1.18, 95% CI = 1.06-1.32) and the family-based transmission disequilibrium test (TDT, p = 0.005, OR = 1.93, 95% CI = 1.21-3.07). The analysis of the combined 2,086 cases and 1,986 controls provided highly significant evidence for the association (p_(trend) = 2.94x10(-6), OR = 1.23, 95% CI = 1.13-1.35). Furthermore, we also found suggestive epistatic effect between rs2269577 and HLA-DRB1*07 allele on the development of vitiligo (p = 0.033). Our subsequent functional study showed that the risk-associated C allele of rs2269577 had a stronger promoter activity than the non-risk G allele, and there was an elevated expression of XBP1 in the lesional skins of patients carrying the risk-associated C allele. Therefore, our study has demonstrated that the transcriptional modulation of XBP1 expression by a germ-line regulatory polymorphism has an impact on the development of vitiligo.

Show MeSH
Related in: MedlinePlus