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Modulation of NKT cells and Th1/Th2 imbalance after alpha-GalCer treatment in progressive load-trained rats.

Ru W, Peijie C - Int. J. Biol. Sci. (2009)

Bottom Line: The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-gamma (IFN-gamma) and interleukin (IL)-4 producing cells.Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1.In Series 2, alpha-galactosylceramide (alpha-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Sports Medicine, Shanghai University of Sport, Shanghai 200438, China.

ABSTRACT

Purpose: The purpose of this study was to determine whether alpha-galactosylceramide (alpha-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance.

Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasing load for 9 weeks. At 36 h and at 7 d after training, groups of rats were euthanized. The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-gamma (IFN-gamma) and interleukin (IL)-4 producing cells.

Results: Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1. The results showed, at 36h after training, that the decrease in Hb, T and C concentration reflected overtraining or excessive exercise. At 7 d after training, NKT cell populations decreased, and a T helper 1/T helper 2 (Th1/Th2) lymphocyte imbalance occurred. In Series 2, alpha-galactosylceramide (alpha-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells.

Conclusion: These data showed that, in addition to Th1/Th2 self-regulation, alpha-GalCer played an important modulatory role in the exercise-induced Th1/Th2 lymphocyte imbalance, which may be correlative with NKT immunoregulatory cells.

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Effect of α-GalCer treatment on NKT cell frequency and IFN-γ and IL-4 producing cells in a week post-training. There are 6 rats in each group. *p<0.05 compared with control. **p<0.01 compared with control. #p<0.05 compared with untreated training group. # #p<0.01 compared with untreated training group.
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Figure 1: Effect of α-GalCer treatment on NKT cell frequency and IFN-γ and IL-4 producing cells in a week post-training. There are 6 rats in each group. *p<0.05 compared with control. **p<0.01 compared with control. #p<0.05 compared with untreated training group. # #p<0.01 compared with untreated training group.

Mentions: In series 2, after training, α-GalCer treatment (twice in the week following training) significant increased the exercise training-induced reduction of NKT cells compared with untreated training group (p < 0.05). Moreover, α-GalCer injection significantly reduced the exercise-induced increase in IL-4 producing cells compared with untreated training group (p < 0.01), while not affecting the number of IFN-γ producing cells (Figure 1). Thus, we observed that α-GalCer injection normalized the levels of NKT cells and IL-4 producing cells in response to exercise training after training.


Modulation of NKT cells and Th1/Th2 imbalance after alpha-GalCer treatment in progressive load-trained rats.

Ru W, Peijie C - Int. J. Biol. Sci. (2009)

Effect of α-GalCer treatment on NKT cell frequency and IFN-γ and IL-4 producing cells in a week post-training. There are 6 rats in each group. *p<0.05 compared with control. **p<0.01 compared with control. #p<0.05 compared with untreated training group. # #p<0.01 compared with untreated training group.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2684679&req=5

Figure 1: Effect of α-GalCer treatment on NKT cell frequency and IFN-γ and IL-4 producing cells in a week post-training. There are 6 rats in each group. *p<0.05 compared with control. **p<0.01 compared with control. #p<0.05 compared with untreated training group. # #p<0.01 compared with untreated training group.
Mentions: In series 2, after training, α-GalCer treatment (twice in the week following training) significant increased the exercise training-induced reduction of NKT cells compared with untreated training group (p < 0.05). Moreover, α-GalCer injection significantly reduced the exercise-induced increase in IL-4 producing cells compared with untreated training group (p < 0.01), while not affecting the number of IFN-γ producing cells (Figure 1). Thus, we observed that α-GalCer injection normalized the levels of NKT cells and IL-4 producing cells in response to exercise training after training.

Bottom Line: The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-gamma (IFN-gamma) and interleukin (IL)-4 producing cells.Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1.In Series 2, alpha-galactosylceramide (alpha-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Sports Medicine, Shanghai University of Sport, Shanghai 200438, China.

ABSTRACT

Purpose: The purpose of this study was to determine whether alpha-galactosylceramide (alpha-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance.

Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasing load for 9 weeks. At 36 h and at 7 d after training, groups of rats were euthanized. The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-gamma (IFN-gamma) and interleukin (IL)-4 producing cells.

Results: Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1. The results showed, at 36h after training, that the decrease in Hb, T and C concentration reflected overtraining or excessive exercise. At 7 d after training, NKT cell populations decreased, and a T helper 1/T helper 2 (Th1/Th2) lymphocyte imbalance occurred. In Series 2, alpha-galactosylceramide (alpha-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells.

Conclusion: These data showed that, in addition to Th1/Th2 self-regulation, alpha-GalCer played an important modulatory role in the exercise-induced Th1/Th2 lymphocyte imbalance, which may be correlative with NKT immunoregulatory cells.

Show MeSH
Related in: MedlinePlus