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A pilot study on acute inflammation and cancer: a new balance between IFN-gamma and TGF-beta in melanoma.

Ma YM, Sun T, Liu YX, Zhao N, Gu Q, Zhang DF, Qie S, Ni CS, Liu Y, Sun BC - J. Exp. Clin. Cancer Res. (2009)

Bottom Line: Recent data have redefined the concept of inflammation as a critical component of tumor progression.In the early phase, inhibitory effects are present.The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, PR China.

ABSTRACT
Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims to observe the impact of a wound on a tumor, to build a new mouse tumor model with a manufactured surgical wound representing acute inflammation, and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth. We focus on the two phases that are present when acute inflammation influences tumor. In the early phase, inhibitory effects are present. The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation. In the latter phase, the inhibited tumor is made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory factor effect on the tumor cells. A pair of cytokines IFN-gamma/TGF-beta established a new balance to protect the tumor from the interference effect of the inflammation. The tumor was made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory effect on the tumor cells. This balance mechanism that occurred in the tumor cells increased proliferation and invasion. In vitro and in vivo experiments have confirmed a new view of clinical surgery that will provide more detailed information on the evaluation of tumors after surgery. This study also provides a better understanding of the relationship between tumor and inflammation, as well as tumor cell attacks on inflammatory factors.

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Related in: MedlinePlus

Determination of the effect of IFN-γ injection on the tumor via tail-vein to validate the IFN-γ released from the wound model. A.) The tumor growth curves showing the double-phase in the IFN-γ injection group, the inhibition phase, and the inhibition missing phase. In the inhibition missing phase, the level of TGF-β increased significantly in the IFN-γ injection group as compared to that in the control (marked by *, p < 0.05). B.) The activity of MMP-2 and MMP-9 as detected by the gelatin zymography analysis showing the decrease in the inhibition phase of the IFN-γ injection group and the significant increase in the inhibition missing phase as compared to the control group (marked by *, p < 0.05).
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Figure 6: Determination of the effect of IFN-γ injection on the tumor via tail-vein to validate the IFN-γ released from the wound model. A.) The tumor growth curves showing the double-phase in the IFN-γ injection group, the inhibition phase, and the inhibition missing phase. In the inhibition missing phase, the level of TGF-β increased significantly in the IFN-γ injection group as compared to that in the control (marked by *, p < 0.05). B.) The activity of MMP-2 and MMP-9 as detected by the gelatin zymography analysis showing the decrease in the inhibition phase of the IFN-γ injection group and the significant increase in the inhibition missing phase as compared to the control group (marked by *, p < 0.05).

Mentions: To investigate the process in which IFN-γ plays an important role in the process of wound inhibition on tumor, a validation experiment was done. We injected IFN-γ into the tail-vein (injection group) to mimic the inflammatory factors from the wound. The results show a similar effect on both the wound group and the injection group. The tumor growth curve showed two phases similar to the curve of the wound group: the inhibition phase (days 5 to 9) and the inhibition missing phase (after day 9). In the inhibition phase, there are no differences on the level of TGF-β between the injection group and the control group. However, in the inhibition missing phase, the level of TGF-β increased significantly both in the serum and the tumor of the injection group as compared to the control group (Figure 6A).


A pilot study on acute inflammation and cancer: a new balance between IFN-gamma and TGF-beta in melanoma.

Ma YM, Sun T, Liu YX, Zhao N, Gu Q, Zhang DF, Qie S, Ni CS, Liu Y, Sun BC - J. Exp. Clin. Cancer Res. (2009)

Determination of the effect of IFN-γ injection on the tumor via tail-vein to validate the IFN-γ released from the wound model. A.) The tumor growth curves showing the double-phase in the IFN-γ injection group, the inhibition phase, and the inhibition missing phase. In the inhibition missing phase, the level of TGF-β increased significantly in the IFN-γ injection group as compared to that in the control (marked by *, p < 0.05). B.) The activity of MMP-2 and MMP-9 as detected by the gelatin zymography analysis showing the decrease in the inhibition phase of the IFN-γ injection group and the significant increase in the inhibition missing phase as compared to the control group (marked by *, p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2683570&req=5

Figure 6: Determination of the effect of IFN-γ injection on the tumor via tail-vein to validate the IFN-γ released from the wound model. A.) The tumor growth curves showing the double-phase in the IFN-γ injection group, the inhibition phase, and the inhibition missing phase. In the inhibition missing phase, the level of TGF-β increased significantly in the IFN-γ injection group as compared to that in the control (marked by *, p < 0.05). B.) The activity of MMP-2 and MMP-9 as detected by the gelatin zymography analysis showing the decrease in the inhibition phase of the IFN-γ injection group and the significant increase in the inhibition missing phase as compared to the control group (marked by *, p < 0.05).
Mentions: To investigate the process in which IFN-γ plays an important role in the process of wound inhibition on tumor, a validation experiment was done. We injected IFN-γ into the tail-vein (injection group) to mimic the inflammatory factors from the wound. The results show a similar effect on both the wound group and the injection group. The tumor growth curve showed two phases similar to the curve of the wound group: the inhibition phase (days 5 to 9) and the inhibition missing phase (after day 9). In the inhibition phase, there are no differences on the level of TGF-β between the injection group and the control group. However, in the inhibition missing phase, the level of TGF-β increased significantly both in the serum and the tumor of the injection group as compared to the control group (Figure 6A).

Bottom Line: Recent data have redefined the concept of inflammation as a critical component of tumor progression.In the early phase, inhibitory effects are present.The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, PR China.

ABSTRACT
Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims to observe the impact of a wound on a tumor, to build a new mouse tumor model with a manufactured surgical wound representing acute inflammation, and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth. We focus on the two phases that are present when acute inflammation influences tumor. In the early phase, inhibitory effects are present. The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation. In the latter phase, the inhibited tumor is made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory factor effect on the tumor cells. A pair of cytokines IFN-gamma/TGF-beta established a new balance to protect the tumor from the interference effect of the inflammation. The tumor was made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory effect on the tumor cells. This balance mechanism that occurred in the tumor cells increased proliferation and invasion. In vitro and in vivo experiments have confirmed a new view of clinical surgery that will provide more detailed information on the evaluation of tumors after surgery. This study also provides a better understanding of the relationship between tumor and inflammation, as well as tumor cell attacks on inflammatory factors.

Show MeSH
Related in: MedlinePlus