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A pilot study on acute inflammation and cancer: a new balance between IFN-gamma and TGF-beta in melanoma.

Ma YM, Sun T, Liu YX, Zhao N, Gu Q, Zhang DF, Qie S, Ni CS, Liu Y, Sun BC - J. Exp. Clin. Cancer Res. (2009)

Bottom Line: Recent data have redefined the concept of inflammation as a critical component of tumor progression.In the early phase, inhibitory effects are present.The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, PR China.

ABSTRACT
Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims to observe the impact of a wound on a tumor, to build a new mouse tumor model with a manufactured surgical wound representing acute inflammation, and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth. We focus on the two phases that are present when acute inflammation influences tumor. In the early phase, inhibitory effects are present. The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation. In the latter phase, the inhibited tumor is made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory factor effect on the tumor cells. A pair of cytokines IFN-gamma/TGF-beta established a new balance to protect the tumor from the interference effect of the inflammation. The tumor was made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory effect on the tumor cells. This balance mechanism that occurred in the tumor cells increased proliferation and invasion. In vitro and in vivo experiments have confirmed a new view of clinical surgery that will provide more detailed information on the evaluation of tumors after surgery. This study also provides a better understanding of the relationship between tumor and inflammation, as well as tumor cell attacks on inflammatory factors.

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To verify whether TGF-β and IFN-γ can enhance melanoma cells invasion by gelatin zymography assay analyzed in vitro and in vivo. A.) B16 cells treated by cytokines, show that IFN-γ can reduce the activity of MMP-2 and MMP-9, which are key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9, giving TGF-β and IFN-γ. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and performed increasing activities on MMP-2 and MMP-9. B.) In vivo animal experiments also showed that there are significant features in day 7; the wound group had significantly lower activities of MMP-2 and MMP-9 compared with the control group from, 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound groups and the control group. (*, p < 0.01)
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Figure 4: To verify whether TGF-β and IFN-γ can enhance melanoma cells invasion by gelatin zymography assay analyzed in vitro and in vivo. A.) B16 cells treated by cytokines, show that IFN-γ can reduce the activity of MMP-2 and MMP-9, which are key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9, giving TGF-β and IFN-γ. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and performed increasing activities on MMP-2 and MMP-9. B.) In vivo animal experiments also showed that there are significant features in day 7; the wound group had significantly lower activities of MMP-2 and MMP-9 compared with the control group from, 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound groups and the control group. (*, p < 0.01)

Mentions: To verify whether TGF-β and IFN-γ can enhance melanoma cell invasion, gelatin zymography assay was used. In the in vitro analysis, IFN-γ can reduce the activity of MMP-2 and MMP-9, which are the key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and increased the activity of MMP-2 and MMP-9 in the T and I group. In vivo animal experiments also showed that there are significant features on day 7, as the wound group had a significantly lower MMP-2 and MMP-9 activity as compared to the control group, from 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound group and the control group (Figure 4).


A pilot study on acute inflammation and cancer: a new balance between IFN-gamma and TGF-beta in melanoma.

Ma YM, Sun T, Liu YX, Zhao N, Gu Q, Zhang DF, Qie S, Ni CS, Liu Y, Sun BC - J. Exp. Clin. Cancer Res. (2009)

To verify whether TGF-β and IFN-γ can enhance melanoma cells invasion by gelatin zymography assay analyzed in vitro and in vivo. A.) B16 cells treated by cytokines, show that IFN-γ can reduce the activity of MMP-2 and MMP-9, which are key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9, giving TGF-β and IFN-γ. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and performed increasing activities on MMP-2 and MMP-9. B.) In vivo animal experiments also showed that there are significant features in day 7; the wound group had significantly lower activities of MMP-2 and MMP-9 compared with the control group from, 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound groups and the control group. (*, p < 0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2683570&req=5

Figure 4: To verify whether TGF-β and IFN-γ can enhance melanoma cells invasion by gelatin zymography assay analyzed in vitro and in vivo. A.) B16 cells treated by cytokines, show that IFN-γ can reduce the activity of MMP-2 and MMP-9, which are key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9, giving TGF-β and IFN-γ. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and performed increasing activities on MMP-2 and MMP-9. B.) In vivo animal experiments also showed that there are significant features in day 7; the wound group had significantly lower activities of MMP-2 and MMP-9 compared with the control group from, 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound groups and the control group. (*, p < 0.01)
Mentions: To verify whether TGF-β and IFN-γ can enhance melanoma cell invasion, gelatin zymography assay was used. In the in vitro analysis, IFN-γ can reduce the activity of MMP-2 and MMP-9, which are the key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and increased the activity of MMP-2 and MMP-9 in the T and I group. In vivo animal experiments also showed that there are significant features on day 7, as the wound group had a significantly lower MMP-2 and MMP-9 activity as compared to the control group, from 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound group and the control group (Figure 4).

Bottom Line: Recent data have redefined the concept of inflammation as a critical component of tumor progression.In the early phase, inhibitory effects are present.The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, PR China.

ABSTRACT
Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims to observe the impact of a wound on a tumor, to build a new mouse tumor model with a manufactured surgical wound representing acute inflammation, and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth. We focus on the two phases that are present when acute inflammation influences tumor. In the early phase, inhibitory effects are present. The process that produces these effects is the functional reaction of IFN-gamma secretions from a wound inflammation. In the latter phase, the inhibited tumor is made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory factor effect on the tumor cells. A pair of cytokines IFN-gamma/TGF-beta established a new balance to protect the tumor from the interference effect of the inflammation. The tumor was made resistant to IFN-gamma through the release of TGF-beta to balance the inflammatory effect on the tumor cells. This balance mechanism that occurred in the tumor cells increased proliferation and invasion. In vitro and in vivo experiments have confirmed a new view of clinical surgery that will provide more detailed information on the evaluation of tumors after surgery. This study also provides a better understanding of the relationship between tumor and inflammation, as well as tumor cell attacks on inflammatory factors.

Show MeSH
Related in: MedlinePlus