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Diverse genotypes of Kaposi's sarcoma associated herpesvirus (KSHV) identified in infant blood infections in African childhood-KS and HIV/AIDS endemic region.

Kasolo FC, Spinks J, Bima H, Bates M, Gompels UA - J. Med. Virol. (2007)

Bottom Line: This current enlarged study analyses blood infections of 200 hospitalized infants (6-34 months age) with symptoms of fever as well as upper respiratory tract infection, diarrhoea, rash or rhinitis.KSHV and HIV viraemia and were prevalent in this group, 22% and 39%, respectively.This supports the interpretation that the acquisition of childhood KSHV infections and subsequent development of KS are due to additional co-factors.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, University Teaching Hospital, University of Zambia Medical School, Lusaka, Zambia.

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Sequence analyses of K1 variable region, VRI loop, identifies genotype diversity in childhood KSHV from African endemic region. The K1 region was PCR amplified from blood DNA, sequenced and aligned against published representatives of K1 genotypes [Zong et al., 1999]. Dashes indicated identity.
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fig01: Sequence analyses of K1 variable region, VRI loop, identifies genotype diversity in childhood KSHV from African endemic region. The K1 region was PCR amplified from blood DNA, sequenced and aligned against published representatives of K1 genotypes [Zong et al., 1999]. Dashes indicated identity.

Mentions: Many samples were at the limit of detection by PCR, which restricted subsequent multiple analyses by nucleotide sequencing. The PCR positive K1 loci was first examined by sequencing. The results showed the K1 A5 genotype, previously identified in infected children and in childhood KS in this region, but also unexpected diversity. The K1 A5 genotype was common 23% (5/22), now linked here with K12 B1 or B2 and K14.1 P genotypes, consistent with previous results for a distinct strain in this region (Table I and Fig. 1), and presenting with respiratory symptoms (4/5). However, also shown were K1–B (4/22), (prevalent in African adult KS) as well as K1–A2, A3, and C3, previously common in adult Mediterranean/Asian/Europe KS (C3, 7/22). There were HIV positive and negative examples of all. The Zambian infant blood KSHV sequences clustered with representative of K1 A, B, C, or D genotypes from adult KS biopsy samples (Fig. 2).


Diverse genotypes of Kaposi's sarcoma associated herpesvirus (KSHV) identified in infant blood infections in African childhood-KS and HIV/AIDS endemic region.

Kasolo FC, Spinks J, Bima H, Bates M, Gompels UA - J. Med. Virol. (2007)

Sequence analyses of K1 variable region, VRI loop, identifies genotype diversity in childhood KSHV from African endemic region. The K1 region was PCR amplified from blood DNA, sequenced and aligned against published representatives of K1 genotypes [Zong et al., 1999]. Dashes indicated identity.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2683451&req=5

fig01: Sequence analyses of K1 variable region, VRI loop, identifies genotype diversity in childhood KSHV from African endemic region. The K1 region was PCR amplified from blood DNA, sequenced and aligned against published representatives of K1 genotypes [Zong et al., 1999]. Dashes indicated identity.
Mentions: Many samples were at the limit of detection by PCR, which restricted subsequent multiple analyses by nucleotide sequencing. The PCR positive K1 loci was first examined by sequencing. The results showed the K1 A5 genotype, previously identified in infected children and in childhood KS in this region, but also unexpected diversity. The K1 A5 genotype was common 23% (5/22), now linked here with K12 B1 or B2 and K14.1 P genotypes, consistent with previous results for a distinct strain in this region (Table I and Fig. 1), and presenting with respiratory symptoms (4/5). However, also shown were K1–B (4/22), (prevalent in African adult KS) as well as K1–A2, A3, and C3, previously common in adult Mediterranean/Asian/Europe KS (C3, 7/22). There were HIV positive and negative examples of all. The Zambian infant blood KSHV sequences clustered with representative of K1 A, B, C, or D genotypes from adult KS biopsy samples (Fig. 2).

Bottom Line: This current enlarged study analyses blood infections of 200 hospitalized infants (6-34 months age) with symptoms of fever as well as upper respiratory tract infection, diarrhoea, rash or rhinitis.KSHV and HIV viraemia and were prevalent in this group, 22% and 39%, respectively.This supports the interpretation that the acquisition of childhood KSHV infections and subsequent development of KS are due to additional co-factors.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, University Teaching Hospital, University of Zambia Medical School, Lusaka, Zambia.

Show MeSH
Related in: MedlinePlus