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Nutrigenomic analysis of diet-gene interactions on functional supplements for weight management.

Lau FC, Bagchi M, Sen C, Roy S, Bagchi D - Curr. Genomics (2008)

Bottom Line: Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic.Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss.The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

View Article: PubMed Central - PubMed

Affiliation: InterHealth Research Center, Benicia, CA, USA.

ABSTRACT
Recent advances in molecular biology combined with the wealth of information generated by the Human Genome Project have fostered the emergence of nutrigenomics, a new discipline in the field of nutritional research. Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic. According to the World Health Organization, more than 60% of the global disease burden will be attributed to chronic disorders associated with obesity by 2020. Meanwhile in the US, the prevalence of obesity has doubled in adults and tripled in children during the past three decades. In this regard, a number of natural dietary supplements and micronutrients have been studied for their potential in weight management. Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss. Utilizing cDNA microarrays, we demonstrated for the first time that HCA-supplementation altered the expression of genes involved in lipolytic and adipogenic pathways in adipocytes from obese women and up-regulated the expression of serotonin receptor gene in the abdominal fat of rats. Similarly, we showed that NBC-supplementation up-regulated the expression of myogenic genes while suppressed the expression of genes that are highly expressed in brown adipose tissue in diabetic obese mice. The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

No MeSH data available.


Related in: MedlinePlus

Flowchart of GeneChip® data analysis scheme. RNA was extracted from each sample and used to assess HCA-SX induced alterations in gene expression in human adipocytes with human genome microarray (U133 v2.0). Raw data were collected by GeneChip® operating software. Data contained in the .cel files were normalized by ArrayAssist® Expression software. The NetAffx ™ software was used to identify fat-specific probes. This subset was derived from the main subset with R 2.2.1 software. Genes whose expression was significantly (P<0.05) altered between the two groups were identified by the dChip software with the testing parameter values set as indicated in the diagram. FDR = false discovery rate. Image used with permission from Mary Ann Liebert, Incorporated [95].
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Figure 5: Flowchart of GeneChip® data analysis scheme. RNA was extracted from each sample and used to assess HCA-SX induced alterations in gene expression in human adipocytes with human genome microarray (U133 v2.0). Raw data were collected by GeneChip® operating software. Data contained in the .cel files were normalized by ArrayAssist® Expression software. The NetAffx ™ software was used to identify fat-specific probes. This subset was derived from the main subset with R 2.2.1 software. Genes whose expression was significantly (P<0.05) altered between the two groups were identified by the dChip software with the testing parameter values set as indicated in the diagram. FDR = false discovery rate. Image used with permission from Mary Ann Liebert, Incorporated [95].

Mentions: In order to gain insight into the genome-wide effects of HCA-SX treatment on human adipocyte gene expression profile, human genome microarrays were used to compare the changes in the transcriptomes between the HCA-SX treated and control human adipocytes. The data acquisition and analysis processed were illustrated in Fig. (5). The human genome microarrays containing a total of 54,676 probe sets per chip were use to screen HCA-SX induced alterations in gene expression profiles of the human adipocytes. HCA-SX treatment caused statistically significant changes in the transcriptome of a very small cluster of genes of which 348 genes were significantly down-regulated while 366 genes were significantly up-regulated as demonstrated in Fig. (6).


Nutrigenomic analysis of diet-gene interactions on functional supplements for weight management.

Lau FC, Bagchi M, Sen C, Roy S, Bagchi D - Curr. Genomics (2008)

Flowchart of GeneChip® data analysis scheme. RNA was extracted from each sample and used to assess HCA-SX induced alterations in gene expression in human adipocytes with human genome microarray (U133 v2.0). Raw data were collected by GeneChip® operating software. Data contained in the .cel files were normalized by ArrayAssist® Expression software. The NetAffx ™ software was used to identify fat-specific probes. This subset was derived from the main subset with R 2.2.1 software. Genes whose expression was significantly (P<0.05) altered between the two groups were identified by the dChip software with the testing parameter values set as indicated in the diagram. FDR = false discovery rate. Image used with permission from Mary Ann Liebert, Incorporated [95].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2682937&req=5

Figure 5: Flowchart of GeneChip® data analysis scheme. RNA was extracted from each sample and used to assess HCA-SX induced alterations in gene expression in human adipocytes with human genome microarray (U133 v2.0). Raw data were collected by GeneChip® operating software. Data contained in the .cel files were normalized by ArrayAssist® Expression software. The NetAffx ™ software was used to identify fat-specific probes. This subset was derived from the main subset with R 2.2.1 software. Genes whose expression was significantly (P<0.05) altered between the two groups were identified by the dChip software with the testing parameter values set as indicated in the diagram. FDR = false discovery rate. Image used with permission from Mary Ann Liebert, Incorporated [95].
Mentions: In order to gain insight into the genome-wide effects of HCA-SX treatment on human adipocyte gene expression profile, human genome microarrays were used to compare the changes in the transcriptomes between the HCA-SX treated and control human adipocytes. The data acquisition and analysis processed were illustrated in Fig. (5). The human genome microarrays containing a total of 54,676 probe sets per chip were use to screen HCA-SX induced alterations in gene expression profiles of the human adipocytes. HCA-SX treatment caused statistically significant changes in the transcriptome of a very small cluster of genes of which 348 genes were significantly down-regulated while 366 genes were significantly up-regulated as demonstrated in Fig. (6).

Bottom Line: Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic.Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss.The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

View Article: PubMed Central - PubMed

Affiliation: InterHealth Research Center, Benicia, CA, USA.

ABSTRACT
Recent advances in molecular biology combined with the wealth of information generated by the Human Genome Project have fostered the emergence of nutrigenomics, a new discipline in the field of nutritional research. Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic. According to the World Health Organization, more than 60% of the global disease burden will be attributed to chronic disorders associated with obesity by 2020. Meanwhile in the US, the prevalence of obesity has doubled in adults and tripled in children during the past three decades. In this regard, a number of natural dietary supplements and micronutrients have been studied for their potential in weight management. Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss. Utilizing cDNA microarrays, we demonstrated for the first time that HCA-supplementation altered the expression of genes involved in lipolytic and adipogenic pathways in adipocytes from obese women and up-regulated the expression of serotonin receptor gene in the abdominal fat of rats. Similarly, we showed that NBC-supplementation up-regulated the expression of myogenic genes while suppressed the expression of genes that are highly expressed in brown adipose tissue in diabetic obese mice. The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

No MeSH data available.


Related in: MedlinePlus