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Nutrigenomic analysis of diet-gene interactions on functional supplements for weight management.

Lau FC, Bagchi M, Sen C, Roy S, Bagchi D - Curr. Genomics (2008)

Bottom Line: Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic.Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss.The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

View Article: PubMed Central - PubMed

Affiliation: InterHealth Research Center, Benicia, CA, USA.

ABSTRACT
Recent advances in molecular biology combined with the wealth of information generated by the Human Genome Project have fostered the emergence of nutrigenomics, a new discipline in the field of nutritional research. Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic. According to the World Health Organization, more than 60% of the global disease burden will be attributed to chronic disorders associated with obesity by 2020. Meanwhile in the US, the prevalence of obesity has doubled in adults and tripled in children during the past three decades. In this regard, a number of natural dietary supplements and micronutrients have been studied for their potential in weight management. Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss. Utilizing cDNA microarrays, we demonstrated for the first time that HCA-supplementation altered the expression of genes involved in lipolytic and adipogenic pathways in adipocytes from obese women and up-regulated the expression of serotonin receptor gene in the abdominal fat of rats. Similarly, we showed that NBC-supplementation up-regulated the expression of myogenic genes while suppressed the expression of genes that are highly expressed in brown adipose tissue in diabetic obese mice. The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

No MeSH data available.


Related in: MedlinePlus

Effect of HCA-SX treatment on the formation of lipid droplets. Differentiated adipocytes from overweight non-diabetic women were cultured with or without HCA-SX (0.5 mg/mL) for 96 hours. After treatment, cells were fixed in 10% buffered formalin, stained with oil red O and images were recorded (A). Large oil droplets (indicated by yellow arrows) in each image were counted with Zeiss Axiovision software (B). Image reproduced with permission from Mary Ann Liebert, Incorporated [95].
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Figure 4: Effect of HCA-SX treatment on the formation of lipid droplets. Differentiated adipocytes from overweight non-diabetic women were cultured with or without HCA-SX (0.5 mg/mL) for 96 hours. After treatment, cells were fixed in 10% buffered formalin, stained with oil red O and images were recorded (A). Large oil droplets (indicated by yellow arrows) in each image were counted with Zeiss Axiovision software (B). Image reproduced with permission from Mary Ann Liebert, Incorporated [95].

Mentions: Defects in the lipolytic process have been linked to human obesity [94]. Roy et al. (2007) sought to investigate the effects of HCA-SX on lipolysis in cultured human adipocytes using biochemical and genomic approaches [95]. Subcutaneous preadipocytes isolated from obese women were allowed to differentiate to adipocytes for two weeks in culture. The effects of HCA-SX on lipid metabolism and transcriptomic profile were examined. HCA-SX treatment at the experimental conditions did not cause any cytotoxicity in the human adipocytes. Fig. (4) showed that HCA-SX treated adipocytic cells exhibited a significant increase in the fat droplet dispersion as compared to the control cells suggesting that HCA-SX treatment potentiated lipolysis through mobilization of triacylglycerol storage.


Nutrigenomic analysis of diet-gene interactions on functional supplements for weight management.

Lau FC, Bagchi M, Sen C, Roy S, Bagchi D - Curr. Genomics (2008)

Effect of HCA-SX treatment on the formation of lipid droplets. Differentiated adipocytes from overweight non-diabetic women were cultured with or without HCA-SX (0.5 mg/mL) for 96 hours. After treatment, cells were fixed in 10% buffered formalin, stained with oil red O and images were recorded (A). Large oil droplets (indicated by yellow arrows) in each image were counted with Zeiss Axiovision software (B). Image reproduced with permission from Mary Ann Liebert, Incorporated [95].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2682937&req=5

Figure 4: Effect of HCA-SX treatment on the formation of lipid droplets. Differentiated adipocytes from overweight non-diabetic women were cultured with or without HCA-SX (0.5 mg/mL) for 96 hours. After treatment, cells were fixed in 10% buffered formalin, stained with oil red O and images were recorded (A). Large oil droplets (indicated by yellow arrows) in each image were counted with Zeiss Axiovision software (B). Image reproduced with permission from Mary Ann Liebert, Incorporated [95].
Mentions: Defects in the lipolytic process have been linked to human obesity [94]. Roy et al. (2007) sought to investigate the effects of HCA-SX on lipolysis in cultured human adipocytes using biochemical and genomic approaches [95]. Subcutaneous preadipocytes isolated from obese women were allowed to differentiate to adipocytes for two weeks in culture. The effects of HCA-SX on lipid metabolism and transcriptomic profile were examined. HCA-SX treatment at the experimental conditions did not cause any cytotoxicity in the human adipocytes. Fig. (4) showed that HCA-SX treated adipocytic cells exhibited a significant increase in the fat droplet dispersion as compared to the control cells suggesting that HCA-SX treatment potentiated lipolysis through mobilization of triacylglycerol storage.

Bottom Line: Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic.Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss.The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

View Article: PubMed Central - PubMed

Affiliation: InterHealth Research Center, Benicia, CA, USA.

ABSTRACT
Recent advances in molecular biology combined with the wealth of information generated by the Human Genome Project have fostered the emergence of nutrigenomics, a new discipline in the field of nutritional research. Nutrigenomics may provide the strategies for the development of safe and effective dietary interventions against the obesity epidemic. According to the World Health Organization, more than 60% of the global disease burden will be attributed to chronic disorders associated with obesity by 2020. Meanwhile in the US, the prevalence of obesity has doubled in adults and tripled in children during the past three decades. In this regard, a number of natural dietary supplements and micronutrients have been studied for their potential in weight management. Among these supplements, (-)-hydroxycitric acid (HCA), a natural extract isolated from the dried fruit rind of Garcinia cambogia, and the micronutrient niacin-bound chromium(III) (NBC) have been shown to be safe and efficacious for weight loss. Utilizing cDNA microarrays, we demonstrated for the first time that HCA-supplementation altered the expression of genes involved in lipolytic and adipogenic pathways in adipocytes from obese women and up-regulated the expression of serotonin receptor gene in the abdominal fat of rats. Similarly, we showed that NBC-supplementation up-regulated the expression of myogenic genes while suppressed the expression of genes that are highly expressed in brown adipose tissue in diabetic obese mice. The potential biological mechanisms underlying the observed beneficial effects of these supplements as elucidated by the state-of-the-art nutrigenomic technologies will be systematically discussed in this review.

No MeSH data available.


Related in: MedlinePlus