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CD152 (CTLA-4) determines CD4 T cell migration in vitro and in vivo.

Knieke K, Hoff H, Maszyna F, Kolar P, Schrage A, Hamann A, Debes GF, Brunner-Weinzierl MC - PLoS ONE (2009)

Bottom Line: We show here that the genetic and serological inactivation of CD152 in Th1 cells reduced migration towards CCL4, CXCL12 and CCL19, but not CXCL9, in a G-protein dependent manner.Importantly, migration of CD152 positive Th1 lymphocytes in in vivo experiments increased more than 200% as compared to CD152 negative counterparts showing that indeed CD152 orchestrates specific migration of selected Th1 cells to sites of inflammation and antigenic challenge in vivo.We show here, that CD152 signaling does not just silence cells, but selects individual ones for migration.

View Article: PubMed Central - PubMed

Affiliation: Experimentelle Pädiatrie, Universitätskinderklinik, Otto-von-Guericke Universität, Magdeburg, Germany.

ABSTRACT

Background: Migration of antigen-experienced T cells to secondary lymphoid organs and the site of antigenic-challenge is a mandatory prerequisite for the precise functioning of adaptive immune responses. The surface molecule CD152 (CTLA-4) is mostly considered as a negative regulator of T cell activation during immune responses. It is currently unknown whether CD152 can also influence chemokine-driven T cell migration.

Methodology/principal findings: We analyzed the consequences of CD152 signaling on Th cell migration using chemotaxis assays in vitro and radioactive cell tracking in vivo. We show here that the genetic and serological inactivation of CD152 in Th1 cells reduced migration towards CCL4, CXCL12 and CCL19, but not CXCL9, in a G-protein dependent manner. In addition, retroviral transduction of CD152 cDNA into CD152 negative cells restored Th1 cell migration. Crosslinking of CD152 together with CD3 and CD28 stimulation on activated Th1 cells increased expression of the chemokine receptors CCR5 and CCR7, which in turn enhanced cell migration. Using sensitive liposome technology, we show that mature dendritic cells but not activated B cells were potent at inducing surface CD152 expression and the CD152-mediated migration-enhancing signals. Importantly, migration of CD152 positive Th1 lymphocytes in in vivo experiments increased more than 200% as compared to CD152 negative counterparts showing that indeed CD152 orchestrates specific migration of selected Th1 cells to sites of inflammation and antigenic challenge in vivo.

Conclusions/significance: We show here, that CD152 signaling does not just silence cells, but selects individual ones for migration. This novel activity of CD152 adds to the already significant role of CD152 in controlling peripheral immune responses by allowing T cells to localize correctly during infection. It also suggests that interference with CD152 signaling provides a tool for altering the cellular composition at sites of inflammation and antigenic challenge.

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CD152 enhances chemotaxis of Th1 cells.(A) Migration of unpolarized T cells. Recall response of CD4+ OVA-specific TCRtg T cells from CD152−/− or CD152+/+ mice was induced by adding 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of recall response cells were analyzed in chemotaxis assays. Bars indicate migrated CD4+ cells as percentage of input cells. (B) Migration of CD152−/− and CD152+/+ T cells in primary stimulation: CD4+ OVA-specific TCRtg T cells were stimulated with 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of primary stimulation cells were analyzed in chemotaxis assays. Bars show the chemotactic index of CD4+ cells. (C) Specific migration of antigen-specific stimulated Th1 cells in a recall response: Primary stimulation and recall response of CD4+CD62L+ OVA-specific TCRtg T cells were performed under Th1 conditions with 1 µg/ml OVA-peptide in the presence of 200 µg/ml neutralizing anti-CD152 Fab fragments or hamster control Fab fragments. Cells were examined in chemotaxis assay on day 6 of recall response. (D) Chemotactic index of a polyclonally induced recall response of CD152−/− or CD152+/+ Th1 cells: Primary stimulation and recall response of splenocytes from CD152−/− and CD152+/+ mice were induced polyclonally (as described in Material and Methods) and CD4+ cells were used for chemotaxis assays on day 4 of recall response. (E) Migration of CD152−/− and CD152+/+ Th1 cells in a recall response is dose dependent: Primary stimulation and recall response of CD4+ cells from TCRtg CD152−/− and CD152+/+ mice were induced antigen-specifically using Th1 conditions with indicated amounts of OVA-peptide and cells were analyzed on day 6 of recall response in chemotaxis assays. All data shown represent one out of 3–4 similar experiments.
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pone-0005702-g001: CD152 enhances chemotaxis of Th1 cells.(A) Migration of unpolarized T cells. Recall response of CD4+ OVA-specific TCRtg T cells from CD152−/− or CD152+/+ mice was induced by adding 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of recall response cells were analyzed in chemotaxis assays. Bars indicate migrated CD4+ cells as percentage of input cells. (B) Migration of CD152−/− and CD152+/+ T cells in primary stimulation: CD4+ OVA-specific TCRtg T cells were stimulated with 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of primary stimulation cells were analyzed in chemotaxis assays. Bars show the chemotactic index of CD4+ cells. (C) Specific migration of antigen-specific stimulated Th1 cells in a recall response: Primary stimulation and recall response of CD4+CD62L+ OVA-specific TCRtg T cells were performed under Th1 conditions with 1 µg/ml OVA-peptide in the presence of 200 µg/ml neutralizing anti-CD152 Fab fragments or hamster control Fab fragments. Cells were examined in chemotaxis assay on day 6 of recall response. (D) Chemotactic index of a polyclonally induced recall response of CD152−/− or CD152+/+ Th1 cells: Primary stimulation and recall response of splenocytes from CD152−/− and CD152+/+ mice were induced polyclonally (as described in Material and Methods) and CD4+ cells were used for chemotaxis assays on day 4 of recall response. (E) Migration of CD152−/− and CD152+/+ Th1 cells in a recall response is dose dependent: Primary stimulation and recall response of CD4+ cells from TCRtg CD152−/− and CD152+/+ mice were induced antigen-specifically using Th1 conditions with indicated amounts of OVA-peptide and cells were analyzed on day 6 of recall response in chemotaxis assays. All data shown represent one out of 3–4 similar experiments.

Mentions: To examine the role of CD152 in T cell migration, we stimulated CD152-deficient (CD152−/−) monoclonal OVA-specific TCRtg CD4+ T cells from OTII mice (TCRtgCD152−/−) and monoclonal OVA-specific TCRtg CD4+ T cells from CD152+/+ OTII mice (TCRtgCD152+/+) with cognate antigen in vitro and tested chemotaxis to the CXCR4 ligand CXCL12, the CCR7 ligand CCL19, and the CCR5 ligand CCL4 in a Transwell chemotaxis assay (Fig. 1A, and data not shown). Similar baseline migration, which was routinely 5–10% in our Transwell migration assays (Figs. 1–5), was detectable for TCRtgCD152+/+ T cells and TCRtgCD152−/− T cells. Under these conditions, no migration towards the CCR5 ligand CCL4 was seen in either population (data not shown). In contrast, TCRtgCD152+/+ T cells migrated 2-fold better in response to CXCL12 than did TCRtgCD152−/− T cells (Fig. 1A). Similarly, migration towards the CCR7 ligand CCL19 was diminished by 70% in TCRtgCD152−/− T cells compared with TCRtgCD152+/+ cells.


CD152 (CTLA-4) determines CD4 T cell migration in vitro and in vivo.

Knieke K, Hoff H, Maszyna F, Kolar P, Schrage A, Hamann A, Debes GF, Brunner-Weinzierl MC - PLoS ONE (2009)

CD152 enhances chemotaxis of Th1 cells.(A) Migration of unpolarized T cells. Recall response of CD4+ OVA-specific TCRtg T cells from CD152−/− or CD152+/+ mice was induced by adding 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of recall response cells were analyzed in chemotaxis assays. Bars indicate migrated CD4+ cells as percentage of input cells. (B) Migration of CD152−/− and CD152+/+ T cells in primary stimulation: CD4+ OVA-specific TCRtg T cells were stimulated with 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of primary stimulation cells were analyzed in chemotaxis assays. Bars show the chemotactic index of CD4+ cells. (C) Specific migration of antigen-specific stimulated Th1 cells in a recall response: Primary stimulation and recall response of CD4+CD62L+ OVA-specific TCRtg T cells were performed under Th1 conditions with 1 µg/ml OVA-peptide in the presence of 200 µg/ml neutralizing anti-CD152 Fab fragments or hamster control Fab fragments. Cells were examined in chemotaxis assay on day 6 of recall response. (D) Chemotactic index of a polyclonally induced recall response of CD152−/− or CD152+/+ Th1 cells: Primary stimulation and recall response of splenocytes from CD152−/− and CD152+/+ mice were induced polyclonally (as described in Material and Methods) and CD4+ cells were used for chemotaxis assays on day 4 of recall response. (E) Migration of CD152−/− and CD152+/+ Th1 cells in a recall response is dose dependent: Primary stimulation and recall response of CD4+ cells from TCRtg CD152−/− and CD152+/+ mice were induced antigen-specifically using Th1 conditions with indicated amounts of OVA-peptide and cells were analyzed on day 6 of recall response in chemotaxis assays. All data shown represent one out of 3–4 similar experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2682661&req=5

pone-0005702-g001: CD152 enhances chemotaxis of Th1 cells.(A) Migration of unpolarized T cells. Recall response of CD4+ OVA-specific TCRtg T cells from CD152−/− or CD152+/+ mice was induced by adding 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of recall response cells were analyzed in chemotaxis assays. Bars indicate migrated CD4+ cells as percentage of input cells. (B) Migration of CD152−/− and CD152+/+ T cells in primary stimulation: CD4+ OVA-specific TCRtg T cells were stimulated with 1 µg/ml OVA323–339 and T cell-depleted APCs. On day 6 of primary stimulation cells were analyzed in chemotaxis assays. Bars show the chemotactic index of CD4+ cells. (C) Specific migration of antigen-specific stimulated Th1 cells in a recall response: Primary stimulation and recall response of CD4+CD62L+ OVA-specific TCRtg T cells were performed under Th1 conditions with 1 µg/ml OVA-peptide in the presence of 200 µg/ml neutralizing anti-CD152 Fab fragments or hamster control Fab fragments. Cells were examined in chemotaxis assay on day 6 of recall response. (D) Chemotactic index of a polyclonally induced recall response of CD152−/− or CD152+/+ Th1 cells: Primary stimulation and recall response of splenocytes from CD152−/− and CD152+/+ mice were induced polyclonally (as described in Material and Methods) and CD4+ cells were used for chemotaxis assays on day 4 of recall response. (E) Migration of CD152−/− and CD152+/+ Th1 cells in a recall response is dose dependent: Primary stimulation and recall response of CD4+ cells from TCRtg CD152−/− and CD152+/+ mice were induced antigen-specifically using Th1 conditions with indicated amounts of OVA-peptide and cells were analyzed on day 6 of recall response in chemotaxis assays. All data shown represent one out of 3–4 similar experiments.
Mentions: To examine the role of CD152 in T cell migration, we stimulated CD152-deficient (CD152−/−) monoclonal OVA-specific TCRtg CD4+ T cells from OTII mice (TCRtgCD152−/−) and monoclonal OVA-specific TCRtg CD4+ T cells from CD152+/+ OTII mice (TCRtgCD152+/+) with cognate antigen in vitro and tested chemotaxis to the CXCR4 ligand CXCL12, the CCR7 ligand CCL19, and the CCR5 ligand CCL4 in a Transwell chemotaxis assay (Fig. 1A, and data not shown). Similar baseline migration, which was routinely 5–10% in our Transwell migration assays (Figs. 1–5), was detectable for TCRtgCD152+/+ T cells and TCRtgCD152−/− T cells. Under these conditions, no migration towards the CCR5 ligand CCL4 was seen in either population (data not shown). In contrast, TCRtgCD152+/+ T cells migrated 2-fold better in response to CXCL12 than did TCRtgCD152−/− T cells (Fig. 1A). Similarly, migration towards the CCR7 ligand CCL19 was diminished by 70% in TCRtgCD152−/− T cells compared with TCRtgCD152+/+ cells.

Bottom Line: We show here that the genetic and serological inactivation of CD152 in Th1 cells reduced migration towards CCL4, CXCL12 and CCL19, but not CXCL9, in a G-protein dependent manner.Importantly, migration of CD152 positive Th1 lymphocytes in in vivo experiments increased more than 200% as compared to CD152 negative counterparts showing that indeed CD152 orchestrates specific migration of selected Th1 cells to sites of inflammation and antigenic challenge in vivo.We show here, that CD152 signaling does not just silence cells, but selects individual ones for migration.

View Article: PubMed Central - PubMed

Affiliation: Experimentelle Pädiatrie, Universitätskinderklinik, Otto-von-Guericke Universität, Magdeburg, Germany.

ABSTRACT

Background: Migration of antigen-experienced T cells to secondary lymphoid organs and the site of antigenic-challenge is a mandatory prerequisite for the precise functioning of adaptive immune responses. The surface molecule CD152 (CTLA-4) is mostly considered as a negative regulator of T cell activation during immune responses. It is currently unknown whether CD152 can also influence chemokine-driven T cell migration.

Methodology/principal findings: We analyzed the consequences of CD152 signaling on Th cell migration using chemotaxis assays in vitro and radioactive cell tracking in vivo. We show here that the genetic and serological inactivation of CD152 in Th1 cells reduced migration towards CCL4, CXCL12 and CCL19, but not CXCL9, in a G-protein dependent manner. In addition, retroviral transduction of CD152 cDNA into CD152 negative cells restored Th1 cell migration. Crosslinking of CD152 together with CD3 and CD28 stimulation on activated Th1 cells increased expression of the chemokine receptors CCR5 and CCR7, which in turn enhanced cell migration. Using sensitive liposome technology, we show that mature dendritic cells but not activated B cells were potent at inducing surface CD152 expression and the CD152-mediated migration-enhancing signals. Importantly, migration of CD152 positive Th1 lymphocytes in in vivo experiments increased more than 200% as compared to CD152 negative counterparts showing that indeed CD152 orchestrates specific migration of selected Th1 cells to sites of inflammation and antigenic challenge in vivo.

Conclusions/significance: We show here, that CD152 signaling does not just silence cells, but selects individual ones for migration. This novel activity of CD152 adds to the already significant role of CD152 in controlling peripheral immune responses by allowing T cells to localize correctly during infection. It also suggests that interference with CD152 signaling provides a tool for altering the cellular composition at sites of inflammation and antigenic challenge.

Show MeSH
Related in: MedlinePlus