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Molecular identification and expression analysis of filaggrin-2, a member of the S100 fused-type protein family.

Wu Z, Hansmann B, Meyer-Hoffert U, Gläser R, Schröder JM - PLoS ONE (2009)

Bottom Line: We found that FLG2 transcripts are present in skin, thymus, tonsils, stomach, testis and placenta.We provide evidences that like filaggrin, FLG2 is initially expressed by upper granular cells, proteolytically processed and deposited in the stratum granulosum and stratum corneum (SC) layers of normal epidermis.Thus, FLG2 and filaggrin may have overlapping and perhaps synergistic roles in the formation of the epidermal barrier, protecting the skin from environmental insults and the escape of moisture by offering precursors of natural moisturizing factors.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University Hospital of Schleswig-Holstein, Kiel, Germany.

ABSTRACT
Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions. Recent genetic studies have shown that mutations within the gene encoding the SFTP filaggrin cause ichthyosis vulgaris and are major predisposing factors for atopic dermatitis. As a vital component of healthy skin, filaggrin is also a precursor of natural moisturizing factors. Here we present the discovery of a member of this family, designated as filaggrin-2 (FLG2) that is expressed in human skin. The FLG2 gene encodes a histidine- and glutamine-rich protein of approximately 248 kDa, which shares common structural features with other SFTP members, in particular filaggrin. We found that FLG2 transcripts are present in skin, thymus, tonsils, stomach, testis and placenta. In cultured primary keratinocytes, FLG2 mRNA expression displayed almost the same kinetics as that of filaggrin following Ca(2+) stimulation, suggesting an important role in molecular regulation of epidermal terminal differentiation. We provide evidences that like filaggrin, FLG2 is initially expressed by upper granular cells, proteolytically processed and deposited in the stratum granulosum and stratum corneum (SC) layers of normal epidermis. Thus, FLG2 and filaggrin may have overlapping and perhaps synergistic roles in the formation of the epidermal barrier, protecting the skin from environmental insults and the escape of moisture by offering precursors of natural moisturizing factors.

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Immunohistochemical analyses of filaggrin-2 expression in skin lesions of psoriasis and atopic dermatitis.Skin biopsies from affected and non-affected sites (upper back) on patients with psoriasis or atopic dermatitis (AD) were stained with anti-FLG2 antibody. A) panel a, lesional psoriatic skin; panel b, non-lesional psoriatic skin; B) panel a, lesional AD skin; panel b, non-lesional AD skin. Representative results of three different patients of each group are shown. Scale bars, 60 µm.
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pone-0005227-g008: Immunohistochemical analyses of filaggrin-2 expression in skin lesions of psoriasis and atopic dermatitis.Skin biopsies from affected and non-affected sites (upper back) on patients with psoriasis or atopic dermatitis (AD) were stained with anti-FLG2 antibody. A) panel a, lesional psoriatic skin; panel b, non-lesional psoriatic skin; B) panel a, lesional AD skin; panel b, non-lesional AD skin. Representative results of three different patients of each group are shown. Scale bars, 60 µm.

Mentions: To characterize FLG2 expression in inflammatory cutaneous diseases, lesional and non-lesional skin of patients suffering from psoriasis and atopic dermatitis were investigated. Psoriatic lesions (n = 3) exhibited decreased FLG2 immunoreactivity, whereas the non-lesional skin of the same patients exhibited a similar staining pattern as that of healthy individuals (Fig. 8A-a and -b). In contrast, there were no obvious staining differences observed between the lesional skin of AD patients and the non-lesional skin of the same patients and healthy individuals (n = 3; Fig. 8B-a and -b).


Molecular identification and expression analysis of filaggrin-2, a member of the S100 fused-type protein family.

Wu Z, Hansmann B, Meyer-Hoffert U, Gläser R, Schröder JM - PLoS ONE (2009)

Immunohistochemical analyses of filaggrin-2 expression in skin lesions of psoriasis and atopic dermatitis.Skin biopsies from affected and non-affected sites (upper back) on patients with psoriasis or atopic dermatitis (AD) were stained with anti-FLG2 antibody. A) panel a, lesional psoriatic skin; panel b, non-lesional psoriatic skin; B) panel a, lesional AD skin; panel b, non-lesional AD skin. Representative results of three different patients of each group are shown. Scale bars, 60 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2668185&req=5

pone-0005227-g008: Immunohistochemical analyses of filaggrin-2 expression in skin lesions of psoriasis and atopic dermatitis.Skin biopsies from affected and non-affected sites (upper back) on patients with psoriasis or atopic dermatitis (AD) were stained with anti-FLG2 antibody. A) panel a, lesional psoriatic skin; panel b, non-lesional psoriatic skin; B) panel a, lesional AD skin; panel b, non-lesional AD skin. Representative results of three different patients of each group are shown. Scale bars, 60 µm.
Mentions: To characterize FLG2 expression in inflammatory cutaneous diseases, lesional and non-lesional skin of patients suffering from psoriasis and atopic dermatitis were investigated. Psoriatic lesions (n = 3) exhibited decreased FLG2 immunoreactivity, whereas the non-lesional skin of the same patients exhibited a similar staining pattern as that of healthy individuals (Fig. 8A-a and -b). In contrast, there were no obvious staining differences observed between the lesional skin of AD patients and the non-lesional skin of the same patients and healthy individuals (n = 3; Fig. 8B-a and -b).

Bottom Line: We found that FLG2 transcripts are present in skin, thymus, tonsils, stomach, testis and placenta.We provide evidences that like filaggrin, FLG2 is initially expressed by upper granular cells, proteolytically processed and deposited in the stratum granulosum and stratum corneum (SC) layers of normal epidermis.Thus, FLG2 and filaggrin may have overlapping and perhaps synergistic roles in the formation of the epidermal barrier, protecting the skin from environmental insults and the escape of moisture by offering precursors of natural moisturizing factors.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University Hospital of Schleswig-Holstein, Kiel, Germany.

ABSTRACT
Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions. Recent genetic studies have shown that mutations within the gene encoding the SFTP filaggrin cause ichthyosis vulgaris and are major predisposing factors for atopic dermatitis. As a vital component of healthy skin, filaggrin is also a precursor of natural moisturizing factors. Here we present the discovery of a member of this family, designated as filaggrin-2 (FLG2) that is expressed in human skin. The FLG2 gene encodes a histidine- and glutamine-rich protein of approximately 248 kDa, which shares common structural features with other SFTP members, in particular filaggrin. We found that FLG2 transcripts are present in skin, thymus, tonsils, stomach, testis and placenta. In cultured primary keratinocytes, FLG2 mRNA expression displayed almost the same kinetics as that of filaggrin following Ca(2+) stimulation, suggesting an important role in molecular regulation of epidermal terminal differentiation. We provide evidences that like filaggrin, FLG2 is initially expressed by upper granular cells, proteolytically processed and deposited in the stratum granulosum and stratum corneum (SC) layers of normal epidermis. Thus, FLG2 and filaggrin may have overlapping and perhaps synergistic roles in the formation of the epidermal barrier, protecting the skin from environmental insults and the escape of moisture by offering precursors of natural moisturizing factors.

Show MeSH
Related in: MedlinePlus