Limits...
QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study.

Hinsch N, Frank M, Döring C, Vorländer C, Hansmann ML - BMC Cancer (2009)

Bottom Line: One major aspect is the lack of typical cytological criteria in well differentiated specimens.New marker molecules, shown by poly- or monoclonal antibodies proved helpful.The specificity of the antibody was validated by western blot analysis In gene expression analysis QPRT was detected as differently expressed between follicular thyroid adenoma and follicular thyroid carcinoma.

View Article: PubMed Central - HTML - PubMed

Affiliation: Senckenbergisches Institut für Pathologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt am Main, Frankfurt, Germany. n.hinsch@em.uni-frankfurt.de

ABSTRACT

Background: The differential diagnosis between follicular thyroid adenoma and minimal invasive follicular thyroid carcinoma is often difficult for several reasons. One major aspect is the lack of typical cytological criteria in well differentiated specimens. New marker molecules, shown by poly- or monoclonal antibodies proved helpful.

Methods: We performed global gene expression analysis of 12 follicular thyroid tumours (4 follicular adenomas, 4 minimal invasive follicular carcinomas and 4 widely invasive follicular carcinomas), followed by immunohistochemical staining of 149 cases. The specificity of the antibody was validated by western blot analysis

Results: In gene expression analysis QPRT was detected as differently expressed between follicular thyroid adenoma and follicular thyroid carcinoma. QPRT protein could be detected by immunohistochemistry in 65% of follicular thyroid carcinomas including minimal invasive variant and only 22% of follicular adenomas.

Conclusion: Consequently, QPRT is a potential new marker for the immunohistochemical screening of follicular thyroid nodules.

Show MeSH

Related in: MedlinePlus

qRT-PCR (A) and western blot analysis (B) of the 3 FTA and FTC presented in figure 2. In qRT-PCR, carcinomas reveal a relative quantity of QPRT-RNA expression between 3,65 and 25,18. The relative quantity of QPRT-RNA expression in adenomas is between 0,13 and 1,00. In western blot analysis, FTC reveal a strong band at 34 kD, while follicular adenomas lack any band. Blotting with actin was performed as loading control. Ad: Adenoma; Ca: Carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2667537&req=5

Figure 3: qRT-PCR (A) and western blot analysis (B) of the 3 FTA and FTC presented in figure 2. In qRT-PCR, carcinomas reveal a relative quantity of QPRT-RNA expression between 3,65 and 25,18. The relative quantity of QPRT-RNA expression in adenomas is between 0,13 and 1,00. In western blot analysis, FTC reveal a strong band at 34 kD, while follicular adenomas lack any band. Blotting with actin was performed as loading control. Ad: Adenoma; Ca: Carcinoma.

Mentions: Western blotting confirmed the specificity of the QPRT-antibody. 3 FTA and 3 FTC were used for western blotting. Only the three FTC, but none of the FTA, showed a band at 34 kD (Figure 3B).


QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study.

Hinsch N, Frank M, Döring C, Vorländer C, Hansmann ML - BMC Cancer (2009)

qRT-PCR (A) and western blot analysis (B) of the 3 FTA and FTC presented in figure 2. In qRT-PCR, carcinomas reveal a relative quantity of QPRT-RNA expression between 3,65 and 25,18. The relative quantity of QPRT-RNA expression in adenomas is between 0,13 and 1,00. In western blot analysis, FTC reveal a strong band at 34 kD, while follicular adenomas lack any band. Blotting with actin was performed as loading control. Ad: Adenoma; Ca: Carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667537&req=5

Figure 3: qRT-PCR (A) and western blot analysis (B) of the 3 FTA and FTC presented in figure 2. In qRT-PCR, carcinomas reveal a relative quantity of QPRT-RNA expression between 3,65 and 25,18. The relative quantity of QPRT-RNA expression in adenomas is between 0,13 and 1,00. In western blot analysis, FTC reveal a strong band at 34 kD, while follicular adenomas lack any band. Blotting with actin was performed as loading control. Ad: Adenoma; Ca: Carcinoma.
Mentions: Western blotting confirmed the specificity of the QPRT-antibody. 3 FTA and 3 FTC were used for western blotting. Only the three FTC, but none of the FTA, showed a band at 34 kD (Figure 3B).

Bottom Line: One major aspect is the lack of typical cytological criteria in well differentiated specimens.New marker molecules, shown by poly- or monoclonal antibodies proved helpful.The specificity of the antibody was validated by western blot analysis In gene expression analysis QPRT was detected as differently expressed between follicular thyroid adenoma and follicular thyroid carcinoma.

View Article: PubMed Central - HTML - PubMed

Affiliation: Senckenbergisches Institut für Pathologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt am Main, Frankfurt, Germany. n.hinsch@em.uni-frankfurt.de

ABSTRACT

Background: The differential diagnosis between follicular thyroid adenoma and minimal invasive follicular thyroid carcinoma is often difficult for several reasons. One major aspect is the lack of typical cytological criteria in well differentiated specimens. New marker molecules, shown by poly- or monoclonal antibodies proved helpful.

Methods: We performed global gene expression analysis of 12 follicular thyroid tumours (4 follicular adenomas, 4 minimal invasive follicular carcinomas and 4 widely invasive follicular carcinomas), followed by immunohistochemical staining of 149 cases. The specificity of the antibody was validated by western blot analysis

Results: In gene expression analysis QPRT was detected as differently expressed between follicular thyroid adenoma and follicular thyroid carcinoma. QPRT protein could be detected by immunohistochemistry in 65% of follicular thyroid carcinomas including minimal invasive variant and only 22% of follicular adenomas.

Conclusion: Consequently, QPRT is a potential new marker for the immunohistochemical screening of follicular thyroid nodules.

Show MeSH
Related in: MedlinePlus