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Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

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Immunohistochemistry of Hsp105 in implantation site and inter-implantation segment. The pregnant rats were killed on Day 6, the implantation sites (D6m) and the inter-implantation segments (D6n) were isolated for preparation of immunohistochemistry. Hsp105 expression in endometrium at the implantation site was much stronger than that at the inter-implantation segment. CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm.
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Figure 4: Immunohistochemistry of Hsp105 in implantation site and inter-implantation segment. The pregnant rats were killed on Day 6, the implantation sites (D6m) and the inter-implantation segments (D6n) were isolated for preparation of immunohistochemistry. Hsp105 expression in endometrium at the implantation site was much stronger than that at the inter-implantation segment. CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm.

Mentions: In order to know whether Hsp105 expression is related to implantation, we analyzed its expression in both implantation site and the inter-implantation segment on day 6 by immunohistochemistry. The results showed that the expression of this protein at the implantation site (Fig. 4, D6m) was much stronger than that in the interimplantation segment (Fig. 4, D6n), as summarized in Table 3.


Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Immunohistochemistry of Hsp105 in implantation site and inter-implantation segment. The pregnant rats were killed on Day 6, the implantation sites (D6m) and the inter-implantation segments (D6n) were isolated for preparation of immunohistochemistry. Hsp105 expression in endometrium at the implantation site was much stronger than that at the inter-implantation segment. CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667524&req=5

Figure 4: Immunohistochemistry of Hsp105 in implantation site and inter-implantation segment. The pregnant rats were killed on Day 6, the implantation sites (D6m) and the inter-implantation segments (D6n) were isolated for preparation of immunohistochemistry. Hsp105 expression in endometrium at the implantation site was much stronger than that at the inter-implantation segment. CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm.
Mentions: In order to know whether Hsp105 expression is related to implantation, we analyzed its expression in both implantation site and the inter-implantation segment on day 6 by immunohistochemistry. The results showed that the expression of this protein at the implantation site (Fig. 4, D6m) was much stronger than that in the interimplantation segment (Fig. 4, D6n), as summarized in Table 3.

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

Show MeSH
Related in: MedlinePlus