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Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

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Immunohistochemistry of Hsp105 during pseudopregnancy. The adult male animals were vasectomized and 14 days later were used to mate with the adult females of the same strain to induce pseudo-pregnancy (pseudo-pregnancy day 1 (PD1) = day of vaginal plug positive). Hsp150 protein was mainly detected in the luminal epithelium on day 1 (PD1). The protein staining was increased in the luminal epithelium and glandular epithelium from day 2 to 3 (PD2, PD3). The protein expression was decreased from day 4 to 7 (PD4, PD5, PD6, PD7). CON, negtive control from day 2 of pseudopregancy; le, luminal epithelium; ge, glandular epithelium; s, stroma. Bar = 200 μm.
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Figure 3: Immunohistochemistry of Hsp105 during pseudopregnancy. The adult male animals were vasectomized and 14 days later were used to mate with the adult females of the same strain to induce pseudo-pregnancy (pseudo-pregnancy day 1 (PD1) = day of vaginal plug positive). Hsp150 protein was mainly detected in the luminal epithelium on day 1 (PD1). The protein staining was increased in the luminal epithelium and glandular epithelium from day 2 to 3 (PD2, PD3). The protein expression was decreased from day 4 to 7 (PD4, PD5, PD6, PD7). CON, negtive control from day 2 of pseudopregancy; le, luminal epithelium; ge, glandular epithelium; s, stroma. Bar = 200 μm.

Mentions: To further confirm specific expression of Hsp105 in relation to implantation, we performed an experiment with pseudopregnant rats. The protein was mainly localized in the luminal epithelium on day 1 (Fig. 3, PD1), with the staining increased in both the luminal and the glandular epithelium on day 2 and 3 (Fig. 3, PD2, PD3), sharply decreased on day 4, and remaining at a low level on day 5 to 7 (Fig. 3, PD4, PD5, PD6, PD7). No peak level expression of this protein was observed in the pseudopregnant uterus. The score of the specific cell staining for Hsp105 in the uterus during pseudopregnancy is summarized in Table 2.


Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Immunohistochemistry of Hsp105 during pseudopregnancy. The adult male animals were vasectomized and 14 days later were used to mate with the adult females of the same strain to induce pseudo-pregnancy (pseudo-pregnancy day 1 (PD1) = day of vaginal plug positive). Hsp150 protein was mainly detected in the luminal epithelium on day 1 (PD1). The protein staining was increased in the luminal epithelium and glandular epithelium from day 2 to 3 (PD2, PD3). The protein expression was decreased from day 4 to 7 (PD4, PD5, PD6, PD7). CON, negtive control from day 2 of pseudopregancy; le, luminal epithelium; ge, glandular epithelium; s, stroma. Bar = 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667524&req=5

Figure 3: Immunohistochemistry of Hsp105 during pseudopregnancy. The adult male animals were vasectomized and 14 days later were used to mate with the adult females of the same strain to induce pseudo-pregnancy (pseudo-pregnancy day 1 (PD1) = day of vaginal plug positive). Hsp150 protein was mainly detected in the luminal epithelium on day 1 (PD1). The protein staining was increased in the luminal epithelium and glandular epithelium from day 2 to 3 (PD2, PD3). The protein expression was decreased from day 4 to 7 (PD4, PD5, PD6, PD7). CON, negtive control from day 2 of pseudopregancy; le, luminal epithelium; ge, glandular epithelium; s, stroma. Bar = 200 μm.
Mentions: To further confirm specific expression of Hsp105 in relation to implantation, we performed an experiment with pseudopregnant rats. The protein was mainly localized in the luminal epithelium on day 1 (Fig. 3, PD1), with the staining increased in both the luminal and the glandular epithelium on day 2 and 3 (Fig. 3, PD2, PD3), sharply decreased on day 4, and remaining at a low level on day 5 to 7 (Fig. 3, PD4, PD5, PD6, PD7). No peak level expression of this protein was observed in the pseudopregnant uterus. The score of the specific cell staining for Hsp105 in the uterus during pseudopregnancy is summarized in Table 2.

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

Show MeSH
Related in: MedlinePlus