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Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

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Western blot analysis of Hsp105 protein in uterus during early pregnancy. A: Representative Western Blot analysis of Hsp 105 protein. Actin protein was used as an internal control. B: The bar graph represents the densitometric analysis of the Hsp 105. The relative intensity was determined by the ratio of Hsp105 protein to its corresponding internal control as measured by densitometry. Data are presented as mean ± SEM (n = 3). Statistical analysis was performed using one-way ANOVA followed by the Fisher's protected least-significance-difference test. Bar with ** is significantly different from D1 of pregnancy (P < 0.01).
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Figure 2: Western blot analysis of Hsp105 protein in uterus during early pregnancy. A: Representative Western Blot analysis of Hsp 105 protein. Actin protein was used as an internal control. B: The bar graph represents the densitometric analysis of the Hsp 105. The relative intensity was determined by the ratio of Hsp105 protein to its corresponding internal control as measured by densitometry. Data are presented as mean ± SEM (n = 3). Statistical analysis was performed using one-way ANOVA followed by the Fisher's protected least-significance-difference test. Bar with ** is significantly different from D1 of pregnancy (P < 0.01).

Mentions: The quantitative change in uterine Hsp105 expression was estimated by Western blot, as shown in Fig. 2. The protein level in the uterus was increased in a time-dependent manner, the highest expression was observed on day5 and day 6, just around the time before and after implantation.


Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

Yuan JX, Xiao LJ, Lu CL, Zhang XS, Liu T, Chen M, Hu ZY, Gao F, Liu YX - Reprod. Biol. Endocrinol. (2009)

Western blot analysis of Hsp105 protein in uterus during early pregnancy. A: Representative Western Blot analysis of Hsp 105 protein. Actin protein was used as an internal control. B: The bar graph represents the densitometric analysis of the Hsp 105. The relative intensity was determined by the ratio of Hsp105 protein to its corresponding internal control as measured by densitometry. Data are presented as mean ± SEM (n = 3). Statistical analysis was performed using one-way ANOVA followed by the Fisher's protected least-significance-difference test. Bar with ** is significantly different from D1 of pregnancy (P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667524&req=5

Figure 2: Western blot analysis of Hsp105 protein in uterus during early pregnancy. A: Representative Western Blot analysis of Hsp 105 protein. Actin protein was used as an internal control. B: The bar graph represents the densitometric analysis of the Hsp 105. The relative intensity was determined by the ratio of Hsp105 protein to its corresponding internal control as measured by densitometry. Data are presented as mean ± SEM (n = 3). Statistical analysis was performed using one-way ANOVA followed by the Fisher's protected least-significance-difference test. Bar with ** is significantly different from D1 of pregnancy (P < 0.01).
Mentions: The quantitative change in uterine Hsp105 expression was estimated by Western blot, as shown in Fig. 2. The protein level in the uterus was increased in a time-dependent manner, the highest expression was observed on day5 and day 6, just around the time before and after implantation.

Bottom Line: Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. yuanjx@ioz.ac.cn

ABSTRACT

Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods: Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results: Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with that obtained by Western blot in the early pregnancy. In contrast, no obvious peak level of Hsp105 was observed in the uterus of pseudopregnant rat on day 5 or day 6. Furthermore, injection of antisense oligodeoxynucleotides to Hsp105 into the rat uterine horn on day 3 of pregnancy obviously suppressed the protein expression as expected and reduced number of the implanted embryos as compared with the control.

Conclusion: Temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation.

Show MeSH
Related in: MedlinePlus