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Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations.

Canales M, Almazán C, Naranjo V, Jongejan F, de la Fuente J - BMC Biotechnol. (2009)

Bottom Line: Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control.Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively.These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, Ciudad Real, Spain. mario.canales@uclm.es

ABSTRACT

Background: The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick infestations. Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris.

Results: Recombinant Ba86 (Israel strain) was used to immunize cattle to test its efficacy for the control of B. annulatus (Mercedes, Texas, USA strain) and B. microplus (Susceptible, Mexico strain) infestations. Bm86 (Gavac and Mozambique strain) and adjuvant/saline were used as positive and negative controls, respectively. Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively. The efficacy of both Ba86 and Bm86 was higher for B. annulatus than for B. microplus. The efficacy of Ba86 was higher for B. annulatus (83.0%) than for B. microplus (71.5%). The efficacy of Bm86 (Gavac; 85.2%) but not Bm86 (Mozambique strain; 70.4%) was higher than that of Ba86 (71.5%) on B. microplus. However, the efficacy of Bm86 (both Gavac and Mozambique strain; 99.6%) was higher than that of Ba86 (83.0%) on B. annulatus.

Conclusion: These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.

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Antibody response in vaccinated cattle. Bovine serum antibody titers to recombinant Ba86 (Israeli strain) and Bm86 (Mozambique strain) antigens were determined by ELISA in cattle vaccinated with Bm86 (Gavac, Cuban Camcord strain), Bm86 (Mozambique strain), Ba86 (Israeli strain) and adjuvant/saline control. Antibody titers in immunized cattle were expressed as the OD450 nm value for the highest serum dilution (1:1000) and compared between vaccinated and control cattle using an ANOVA test (*P < 0.05). The time of vaccination shots (arrows) and tick infestation are indicated.
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Figure 1: Antibody response in vaccinated cattle. Bovine serum antibody titers to recombinant Ba86 (Israeli strain) and Bm86 (Mozambique strain) antigens were determined by ELISA in cattle vaccinated with Bm86 (Gavac, Cuban Camcord strain), Bm86 (Mozambique strain), Ba86 (Israeli strain) and adjuvant/saline control. Antibody titers in immunized cattle were expressed as the OD450 nm value for the highest serum dilution (1:1000) and compared between vaccinated and control cattle using an ANOVA test (*P < 0.05). The time of vaccination shots (arrows) and tick infestation are indicated.

Mentions: This is the first report on the protective capacity of recombinant Ba86 for the control of cattle tick infestations. To evaluate the protective capacity of Ba86 against B. microplus and B. annulatus infestations, cattle were vaccinated with the recombinant protein and compared to cattle vaccinated with two Bm86 preparations and adjuvant/saline control. The vaccinated animals but not the controls developed antibodies against recombinant proteins (Fig. 1). The antibody titers were similar for all groups when measured against Ba86 and Bm86 antigens, thus reflecting the presence of common antigenic epitopes between both proteins (ref. [23] and Fig. 2). The antibody titers in cattle vaccinated with Ba86 and Bm86 (Mozambique strain) were similar and higher than those in animals vaccinated with Bm86 (Gavac) (Fig. 1). As in previous experiments [11,20], except for animals vaccinated with Bm86 (Gavac), antibody titers increased after successive vaccinations and reached a peak two weeks after the third vaccination shot. The differences in the antibody response elicited by Bm86 (Gavac) and Bm86 (Mozambique strain) antigens could be attributed to differences in vaccine preparations (the Bm86 in Gavac was expressed in P. pastoris as membrane-bound while the Bm86 of the Mozambique strain was secreted in P. pastoris; ref. 23] and/or to other unknown factors such as cattle physiological status that have been demonstrated to affect cattle antibody response to Bm86 [14]. Additionally, as discussed bellow, Bm86 polymorphisms between Gavac and Mozambique strain antigens may affect antigen processing and immune response after vaccination [19].


Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations.

Canales M, Almazán C, Naranjo V, Jongejan F, de la Fuente J - BMC Biotechnol. (2009)

Antibody response in vaccinated cattle. Bovine serum antibody titers to recombinant Ba86 (Israeli strain) and Bm86 (Mozambique strain) antigens were determined by ELISA in cattle vaccinated with Bm86 (Gavac, Cuban Camcord strain), Bm86 (Mozambique strain), Ba86 (Israeli strain) and adjuvant/saline control. Antibody titers in immunized cattle were expressed as the OD450 nm value for the highest serum dilution (1:1000) and compared between vaccinated and control cattle using an ANOVA test (*P < 0.05). The time of vaccination shots (arrows) and tick infestation are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667501&req=5

Figure 1: Antibody response in vaccinated cattle. Bovine serum antibody titers to recombinant Ba86 (Israeli strain) and Bm86 (Mozambique strain) antigens were determined by ELISA in cattle vaccinated with Bm86 (Gavac, Cuban Camcord strain), Bm86 (Mozambique strain), Ba86 (Israeli strain) and adjuvant/saline control. Antibody titers in immunized cattle were expressed as the OD450 nm value for the highest serum dilution (1:1000) and compared between vaccinated and control cattle using an ANOVA test (*P < 0.05). The time of vaccination shots (arrows) and tick infestation are indicated.
Mentions: This is the first report on the protective capacity of recombinant Ba86 for the control of cattle tick infestations. To evaluate the protective capacity of Ba86 against B. microplus and B. annulatus infestations, cattle were vaccinated with the recombinant protein and compared to cattle vaccinated with two Bm86 preparations and adjuvant/saline control. The vaccinated animals but not the controls developed antibodies against recombinant proteins (Fig. 1). The antibody titers were similar for all groups when measured against Ba86 and Bm86 antigens, thus reflecting the presence of common antigenic epitopes between both proteins (ref. [23] and Fig. 2). The antibody titers in cattle vaccinated with Ba86 and Bm86 (Mozambique strain) were similar and higher than those in animals vaccinated with Bm86 (Gavac) (Fig. 1). As in previous experiments [11,20], except for animals vaccinated with Bm86 (Gavac), antibody titers increased after successive vaccinations and reached a peak two weeks after the third vaccination shot. The differences in the antibody response elicited by Bm86 (Gavac) and Bm86 (Mozambique strain) antigens could be attributed to differences in vaccine preparations (the Bm86 in Gavac was expressed in P. pastoris as membrane-bound while the Bm86 of the Mozambique strain was secreted in P. pastoris; ref. 23] and/or to other unknown factors such as cattle physiological status that have been demonstrated to affect cattle antibody response to Bm86 [14]. Additionally, as discussed bellow, Bm86 polymorphisms between Gavac and Mozambique strain antigens may affect antigen processing and immune response after vaccination [19].

Bottom Line: Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control.Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively.These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, Ciudad Real, Spain. mario.canales@uclm.es

ABSTRACT

Background: The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick infestations. Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris.

Results: Recombinant Ba86 (Israel strain) was used to immunize cattle to test its efficacy for the control of B. annulatus (Mercedes, Texas, USA strain) and B. microplus (Susceptible, Mexico strain) infestations. Bm86 (Gavac and Mozambique strain) and adjuvant/saline were used as positive and negative controls, respectively. Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively. The efficacy of both Ba86 and Bm86 was higher for B. annulatus than for B. microplus. The efficacy of Ba86 was higher for B. annulatus (83.0%) than for B. microplus (71.5%). The efficacy of Bm86 (Gavac; 85.2%) but not Bm86 (Mozambique strain; 70.4%) was higher than that of Ba86 (71.5%) on B. microplus. However, the efficacy of Bm86 (both Gavac and Mozambique strain; 99.6%) was higher than that of Ba86 (83.0%) on B. annulatus.

Conclusion: These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.

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Related in: MedlinePlus