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A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera.

Dong DJ, Wang JX, Zhao XF - BMC Dev. Biol. (2009)

Bottom Line: Ha-eIF5C expression was upregulated by 20-hydroxyecdysone (20E).Furthermore, the transcription of Ha-eIF5C was down regulated after silencing of ecdysteroid receptor (EcR) or Ultraspiracle protein (USP) by RNAi.These results suggested that during metamorphosis of the cotton bollworm, Ha-eIF5C was upregulated by 20E through the EcR and USP transcription factors.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China. dujuandong@yahoo.com.cn

ABSTRACT

Background: The orthologs of eukaryotic initiation factor 5C (eIF5C) are essential to the initiation of protein translation, and their regulation during development is not well known.

Results: A cDNA encoding a polypeptide of 419 amino acids containing an N-terminal leucine zipper motif and a C-terminal eIF5C domain was cloned from metamorphic larvae of Helicoverpa armigera. It was subsequently named Ha-eIF5C. Quantitative real-time PCR (QRT-PCR) revealed a high expression of the mRNA of Ha-eIF5C in the head-thorax, integument, midgut, and fat body during metamorphosis. Immunohistochemistry suggested that Ha-eIF5C was distributed into both the cytoplasm and the nucleus in the midgut, fat body and integument. Ha-eIF5C expression was upregulated by 20-hydroxyecdysone (20E). Furthermore, the transcription of Ha-eIF5C was down regulated after silencing of ecdysteroid receptor (EcR) or Ultraspiracle protein (USP) by RNAi.

Conclusion: These results suggested that during metamorphosis of the cotton bollworm, Ha-eIF5C was upregulated by 20E through the EcR and USP transcription factors.

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Multiple alignments of Ha-eIF5C with other insect eIF5C. eIF5C from A. aegypti XP_001655819, A. mellifera XP_395256, B. mori NP_001091797 and D. melanogaster Q9VNE2. The numbers on the right indicate the amino acid position of different sequences. Identical amino acids are shaded in black. Other conserved, but not consensus amino acids, are shaded in grey.
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Figure 2: Multiple alignments of Ha-eIF5C with other insect eIF5C. eIF5C from A. aegypti XP_001655819, A. mellifera XP_395256, B. mori NP_001091797 and D. melanogaster Q9VNE2. The numbers on the right indicate the amino acid position of different sequences. Identical amino acids are shaded in black. Other conserved, but not consensus amino acids, are shaded in grey.

Mentions: The result of the BLASTX analysis suggests that Ha-eIF5C has certain similarities to various genes, including eIF5C from Bombyx mori (88%), eIF5C from Apis mellifera (68%), eIF5C from Aedes aegypti (66%) and eIF5C from D. melanogaster (63%) (Fig. 2). SMART predicted that Ha-eIF5C protein contains a C-terminal eIF5C domain (326–411 aa) and an N-terminal leucine zipper motif (39–60 aa).


A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera.

Dong DJ, Wang JX, Zhao XF - BMC Dev. Biol. (2009)

Multiple alignments of Ha-eIF5C with other insect eIF5C. eIF5C from A. aegypti XP_001655819, A. mellifera XP_395256, B. mori NP_001091797 and D. melanogaster Q9VNE2. The numbers on the right indicate the amino acid position of different sequences. Identical amino acids are shaded in black. Other conserved, but not consensus amino acids, are shaded in grey.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667495&req=5

Figure 2: Multiple alignments of Ha-eIF5C with other insect eIF5C. eIF5C from A. aegypti XP_001655819, A. mellifera XP_395256, B. mori NP_001091797 and D. melanogaster Q9VNE2. The numbers on the right indicate the amino acid position of different sequences. Identical amino acids are shaded in black. Other conserved, but not consensus amino acids, are shaded in grey.
Mentions: The result of the BLASTX analysis suggests that Ha-eIF5C has certain similarities to various genes, including eIF5C from Bombyx mori (88%), eIF5C from Apis mellifera (68%), eIF5C from Aedes aegypti (66%) and eIF5C from D. melanogaster (63%) (Fig. 2). SMART predicted that Ha-eIF5C protein contains a C-terminal eIF5C domain (326–411 aa) and an N-terminal leucine zipper motif (39–60 aa).

Bottom Line: Ha-eIF5C expression was upregulated by 20-hydroxyecdysone (20E).Furthermore, the transcription of Ha-eIF5C was down regulated after silencing of ecdysteroid receptor (EcR) or Ultraspiracle protein (USP) by RNAi.These results suggested that during metamorphosis of the cotton bollworm, Ha-eIF5C was upregulated by 20E through the EcR and USP transcription factors.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China. dujuandong@yahoo.com.cn

ABSTRACT

Background: The orthologs of eukaryotic initiation factor 5C (eIF5C) are essential to the initiation of protein translation, and their regulation during development is not well known.

Results: A cDNA encoding a polypeptide of 419 amino acids containing an N-terminal leucine zipper motif and a C-terminal eIF5C domain was cloned from metamorphic larvae of Helicoverpa armigera. It was subsequently named Ha-eIF5C. Quantitative real-time PCR (QRT-PCR) revealed a high expression of the mRNA of Ha-eIF5C in the head-thorax, integument, midgut, and fat body during metamorphosis. Immunohistochemistry suggested that Ha-eIF5C was distributed into both the cytoplasm and the nucleus in the midgut, fat body and integument. Ha-eIF5C expression was upregulated by 20-hydroxyecdysone (20E). Furthermore, the transcription of Ha-eIF5C was down regulated after silencing of ecdysteroid receptor (EcR) or Ultraspiracle protein (USP) by RNAi.

Conclusion: These results suggested that during metamorphosis of the cotton bollworm, Ha-eIF5C was upregulated by 20E through the EcR and USP transcription factors.

Show MeSH