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A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case.

Roth S, Fromm B, Gäde G, Predel R - BMC Evol. Biol. (2009)

Bottom Line: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved.When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Zoology, University of Jena, Erbertstrasse, Germany. steffen.roth@macnews.de

ABSTRACT

Background: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed.

Results: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.

Conclusion: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

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Related in: MedlinePlus

MALDI-TOF mass spectra (neuropeptide mass fingerprints) from single thoracic PSO preparations of three Blaberus/Eublaberus species, representing FMRFamide related peptides which accumulate in the neurohaemal organs of insects (see Predel et al. 2004). The selected species were not distinguishable by screening the CAPA peptides from abdominal PSOs. All ion signals different from those of B. craniifer are marked. Such fingerprint data exist from all neurohaemal organs of all cockroach species investigated.
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Figure 5: MALDI-TOF mass spectra (neuropeptide mass fingerprints) from single thoracic PSO preparations of three Blaberus/Eublaberus species, representing FMRFamide related peptides which accumulate in the neurohaemal organs of insects (see Predel et al. 2004). The selected species were not distinguishable by screening the CAPA peptides from abdominal PSOs. All ion signals different from those of B. craniifer are marked. Such fingerprint data exist from all neurohaemal organs of all cockroach species investigated.

Mentions: From a single cockroach, P. americana, roughly 80 neuropeptides have been elucidated by biochemical methods in recent years. Today, most of these peptides can be identified by mass spectrometric techniques as described in this manuscript, which makes these peptides generally suitable for phylogenetic studies. Fast evolving neuropeptides such as FMRFamides [57] can provide phylogenetic information at the generic level (see Figure 5) [58] but are not suitable for studying the deep level relationships of higher taxa within an insect order because the homology of such peptide copies among far related taxa can be difficult to assess. Other peptide families with multiple members, such as tachykinin-related peptides, pyrokinins, and allatostatins [59] are likely to be most suitable for the incorporation in phylogenetic analyses. These peptide families are represented by more than 30 paralogues in P. americana. Previous experiments have already shown that members of the Blattidae, which cannot be further separated from each other by the analysis of CAPA peptides, AKHs, and sulfakinins, are clearly distinguishable if species-specific pyrokinin sequences are identified [23]. These findings confirm that even short neuropeptide sequences of insects are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.


A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case.

Roth S, Fromm B, Gäde G, Predel R - BMC Evol. Biol. (2009)

MALDI-TOF mass spectra (neuropeptide mass fingerprints) from single thoracic PSO preparations of three Blaberus/Eublaberus species, representing FMRFamide related peptides which accumulate in the neurohaemal organs of insects (see Predel et al. 2004). The selected species were not distinguishable by screening the CAPA peptides from abdominal PSOs. All ion signals different from those of B. craniifer are marked. Such fingerprint data exist from all neurohaemal organs of all cockroach species investigated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667406&req=5

Figure 5: MALDI-TOF mass spectra (neuropeptide mass fingerprints) from single thoracic PSO preparations of three Blaberus/Eublaberus species, representing FMRFamide related peptides which accumulate in the neurohaemal organs of insects (see Predel et al. 2004). The selected species were not distinguishable by screening the CAPA peptides from abdominal PSOs. All ion signals different from those of B. craniifer are marked. Such fingerprint data exist from all neurohaemal organs of all cockroach species investigated.
Mentions: From a single cockroach, P. americana, roughly 80 neuropeptides have been elucidated by biochemical methods in recent years. Today, most of these peptides can be identified by mass spectrometric techniques as described in this manuscript, which makes these peptides generally suitable for phylogenetic studies. Fast evolving neuropeptides such as FMRFamides [57] can provide phylogenetic information at the generic level (see Figure 5) [58] but are not suitable for studying the deep level relationships of higher taxa within an insect order because the homology of such peptide copies among far related taxa can be difficult to assess. Other peptide families with multiple members, such as tachykinin-related peptides, pyrokinins, and allatostatins [59] are likely to be most suitable for the incorporation in phylogenetic analyses. These peptide families are represented by more than 30 paralogues in P. americana. Previous experiments have already shown that members of the Blattidae, which cannot be further separated from each other by the analysis of CAPA peptides, AKHs, and sulfakinins, are clearly distinguishable if species-specific pyrokinin sequences are identified [23]. These findings confirm that even short neuropeptide sequences of insects are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

Bottom Line: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved.When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Zoology, University of Jena, Erbertstrasse, Germany. steffen.roth@macnews.de

ABSTRACT

Background: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed.

Results: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.

Conclusion: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

Show MeSH
Related in: MedlinePlus