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A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case.

Roth S, Fromm B, Gäde G, Predel R - BMC Evol. Biol. (2009)

Bottom Line: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved.When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Zoology, University of Jena, Erbertstrasse, Germany. steffen.roth@macnews.de

ABSTRACT

Background: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed.

Results: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.

Conclusion: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

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Related in: MedlinePlus

Comparison of MALDI-TOF mass spectra (mass range 1000–2000 Da) of single abdominal PSO preparations of Blaberus craniifer and Bantua robusta (= peptide hormone fingerprint). Only few abundant substances are detectable. Underlying sequences were used for phylogenetic analyses.
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Figure 1: Comparison of MALDI-TOF mass spectra (mass range 1000–2000 Da) of single abdominal PSO preparations of Blaberus craniifer and Bantua robusta (= peptide hormone fingerprint). Only few abundant substances are detectable. Underlying sequences were used for phylogenetic analyses.

Mentions: Direct mass spectrometric analysis of abdominal PSO preparations of single specimens (examples given in Figures 1, 2) revealed complete sequences of CAPA peptides from 61 cockroach/termite species. The species list covers major taxa of cockroaches (Blattidae, Polyphagidae, Cryptocercidae, Blaberidae, Blattellidae) and the termite Mastotermes darwiniensis. From most species, three CAPA-periviscerokinins (PVKs), and a single CAPA-pyrokinin (PK) were sequenced. Cryptocercus and the blattellid cockroaches Symploce pallens and Loboptera decipiens express only two different PVKs. A fourth PVK (designated PVK-4) was found in the Madagascan Blaberidae and the Table Mountain cockroach Aptera fusca (for sequences see [48]. These PVK-4 peptides, whose sequences suggest an internal gene duplication of PVK-1 (Elliptorhina, Gromphadorhina, Princisia) or PVK-2 (Aptera), did not influence the topology of phylogenetic trees and were not included in the final alignments. The average size of the PVKs was 11 amino acids (aa) and that of the PK was 17 aa. Sequences of the CAPA peptides were combined for each species and aligned (Table 1). With the inclusion of gaps and sequences of the outgroup species (Locusta migratoria and Drosophila melanogaster), the alignment resulted in 58 characters. Thirteen characters were constant, 12 variable characters were parsimony-uninformative, and 33 variable characters were parsimony-informative. The sequence of PVK-2 was found to be highly conserved and did not contain phylogenetically informative substitutions.


A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case.

Roth S, Fromm B, Gäde G, Predel R - BMC Evol. Biol. (2009)

Comparison of MALDI-TOF mass spectra (mass range 1000–2000 Da) of single abdominal PSO preparations of Blaberus craniifer and Bantua robusta (= peptide hormone fingerprint). Only few abundant substances are detectable. Underlying sequences were used for phylogenetic analyses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667406&req=5

Figure 1: Comparison of MALDI-TOF mass spectra (mass range 1000–2000 Da) of single abdominal PSO preparations of Blaberus craniifer and Bantua robusta (= peptide hormone fingerprint). Only few abundant substances are detectable. Underlying sequences were used for phylogenetic analyses.
Mentions: Direct mass spectrometric analysis of abdominal PSO preparations of single specimens (examples given in Figures 1, 2) revealed complete sequences of CAPA peptides from 61 cockroach/termite species. The species list covers major taxa of cockroaches (Blattidae, Polyphagidae, Cryptocercidae, Blaberidae, Blattellidae) and the termite Mastotermes darwiniensis. From most species, three CAPA-periviscerokinins (PVKs), and a single CAPA-pyrokinin (PK) were sequenced. Cryptocercus and the blattellid cockroaches Symploce pallens and Loboptera decipiens express only two different PVKs. A fourth PVK (designated PVK-4) was found in the Madagascan Blaberidae and the Table Mountain cockroach Aptera fusca (for sequences see [48]. These PVK-4 peptides, whose sequences suggest an internal gene duplication of PVK-1 (Elliptorhina, Gromphadorhina, Princisia) or PVK-2 (Aptera), did not influence the topology of phylogenetic trees and were not included in the final alignments. The average size of the PVKs was 11 amino acids (aa) and that of the PK was 17 aa. Sequences of the CAPA peptides were combined for each species and aligned (Table 1). With the inclusion of gaps and sequences of the outgroup species (Locusta migratoria and Drosophila melanogaster), the alignment resulted in 58 characters. Thirteen characters were constant, 12 variable characters were parsimony-uninformative, and 33 variable characters were parsimony-informative. The sequence of PVK-2 was found to be highly conserved and did not contain phylogenetically informative substitutions.

Bottom Line: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved.When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Zoology, University of Jena, Erbertstrasse, Germany. steffen.roth@macnews.de

ABSTRACT

Background: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed.

Results: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably.

Conclusion: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.

Show MeSH
Related in: MedlinePlus