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Burn model for in vivo investigations of microcirculatory changes.

Goertz O, Vogelpohl J, Jettkant B, Daigeler A, Steinau HU, Steinstraesser L, Langer S - Eplasty (2009)

Bottom Line: Besides the administration of physiologic saline, local disinfection, and symptomatic medications, no causal therapy is known to reduce the tissue damage and accelerate wound healing.The nonperfused area decreased during the observed period and perfusion was almost completely due to angiogenesis at day 14.The formation of edema occurred immediately postburn and decreased during the following observation time.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Buerkle-de-la-Camp-Platz 1, Bochum 44789, Germany. ole.goertz@rub.de

ABSTRACT

Objective: The treatment of burns remains a challenge due to the associated high morbidity and mortality. Besides the administration of physiologic saline, local disinfection, and symptomatic medications, no causal therapy is known to reduce the tissue damage and accelerate wound healing. The aim of the study was to develop a reliable burn model that allows for reproducible quantitative in vivo analysis of the microcirculation, angiogenesis, and leukocyte endothelium interaction after burn injury.

Methods: Experiments were carried out on male hairless mice (n = 9). Full-thickness burns were inflicted with a hot air jet without any contact to the tissue (117 +/- 2.1 degrees C for 1 second; burn area: 1.3 mm(2)). Intravital fluorescent microscopy, in combination with FITC-dextran as plasma marker, was used to assess microcirculatory standard parameters; leukocytes were stained with rhodamine 6G. Values were obtained before, immediately after, as well as at days 1, 3, 7, and 14 postburn.

Results: The nonperfused area decreased during the observed period and perfusion was almost completely due to angiogenesis at day 14. No posttraumatic expansion of the nonperfused area after 24 hours could be observed. Leukocyte endothelium interaction showed its maximum 24 hours postburn. The formation of edema occurred immediately postburn and decreased during the following observation time.

Conclusion: The developed burn model allows a reproducible assessment with significant results of the microcirculation, angiogenesis, and leukocyte endothelium interaction without causing mechanical damage to the tissue; therefore, this model qualifies for the further investigations of interventional drugs to decrease the effects of burn injury.

No MeSH data available.


Related in: MedlinePlus

Burn device. Drawing of the experimental setup with the mouse under inhalation anesthesia placed on an acryl-glass platform with the left ear spread out by 3 microsurgical loops. The ear was exposed to an air jet for 1 second at a temperature of 117°C. The distance between the air tube and the surface of the ear is 1 mm.
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Figure 1: Burn device. Drawing of the experimental setup with the mouse under inhalation anesthesia placed on an acryl-glass platform with the left ear spread out by 3 microsurgical loops. The ear was exposed to an air jet for 1 second at a temperature of 117°C. The distance between the air tube and the surface of the ear is 1 mm.

Mentions: The full-thickness burn was inflicted on the dorsal side of the ear with a specially designed device, inducing a defined burn injury by a hot air jet without any tissue contact (Fig 1, 117 ± 2.1°C for 1 second; distance between the ear and the air jet = 1 mm; burn area of approximately 1.3 mm2).


Burn model for in vivo investigations of microcirculatory changes.

Goertz O, Vogelpohl J, Jettkant B, Daigeler A, Steinau HU, Steinstraesser L, Langer S - Eplasty (2009)

Burn device. Drawing of the experimental setup with the mouse under inhalation anesthesia placed on an acryl-glass platform with the left ear spread out by 3 microsurgical loops. The ear was exposed to an air jet for 1 second at a temperature of 117°C. The distance between the air tube and the surface of the ear is 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2667331&req=5

Figure 1: Burn device. Drawing of the experimental setup with the mouse under inhalation anesthesia placed on an acryl-glass platform with the left ear spread out by 3 microsurgical loops. The ear was exposed to an air jet for 1 second at a temperature of 117°C. The distance between the air tube and the surface of the ear is 1 mm.
Mentions: The full-thickness burn was inflicted on the dorsal side of the ear with a specially designed device, inducing a defined burn injury by a hot air jet without any tissue contact (Fig 1, 117 ± 2.1°C for 1 second; distance between the ear and the air jet = 1 mm; burn area of approximately 1.3 mm2).

Bottom Line: Besides the administration of physiologic saline, local disinfection, and symptomatic medications, no causal therapy is known to reduce the tissue damage and accelerate wound healing.The nonperfused area decreased during the observed period and perfusion was almost completely due to angiogenesis at day 14.The formation of edema occurred immediately postburn and decreased during the following observation time.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr University Bochum, Buerkle-de-la-Camp-Platz 1, Bochum 44789, Germany. ole.goertz@rub.de

ABSTRACT

Objective: The treatment of burns remains a challenge due to the associated high morbidity and mortality. Besides the administration of physiologic saline, local disinfection, and symptomatic medications, no causal therapy is known to reduce the tissue damage and accelerate wound healing. The aim of the study was to develop a reliable burn model that allows for reproducible quantitative in vivo analysis of the microcirculation, angiogenesis, and leukocyte endothelium interaction after burn injury.

Methods: Experiments were carried out on male hairless mice (n = 9). Full-thickness burns were inflicted with a hot air jet without any contact to the tissue (117 +/- 2.1 degrees C for 1 second; burn area: 1.3 mm(2)). Intravital fluorescent microscopy, in combination with FITC-dextran as plasma marker, was used to assess microcirculatory standard parameters; leukocytes were stained with rhodamine 6G. Values were obtained before, immediately after, as well as at days 1, 3, 7, and 14 postburn.

Results: The nonperfused area decreased during the observed period and perfusion was almost completely due to angiogenesis at day 14. No posttraumatic expansion of the nonperfused area after 24 hours could be observed. Leukocyte endothelium interaction showed its maximum 24 hours postburn. The formation of edema occurred immediately postburn and decreased during the following observation time.

Conclusion: The developed burn model allows a reproducible assessment with significant results of the microcirculation, angiogenesis, and leukocyte endothelium interaction without causing mechanical damage to the tissue; therefore, this model qualifies for the further investigations of interventional drugs to decrease the effects of burn injury.

No MeSH data available.


Related in: MedlinePlus