Limits...
The carotenogenesis pathway via the isoprenoid-beta-carotene interference approach in a new strain of Dunaliella salina isolated from Baja California Mexico.

Paniagua-Michel J, Capa-Robles W, Olmos-Soto J, Gutierrez-Millan LE - Mar Drugs (2009)

Bottom Line: When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 microM fosmidomycin, carotenogenesis and the synthesis of beta-carotene were interrupted after two days in cultured D. salina cells.This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina.The effect at the level of proteins and RNA was not evident.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biotechnology, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada 22860, México. jpaniagu@cicese.mx

ABSTRACT
D. salina is one of the recognized natural sources to produce beta-carotene, and an useful model for studying the role of inhibitors and enhancers of carotenogenesis. However there is little information in D. salina regarding whether the isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective inhibitors which in turn may further contribute to elucidate the early steps of carotenogenesis and biosynthesis of beta-carotene. In this study, Dunaliella salina (BC02) isolated from La Salina BC Mexico, was subjected to the method of isoprenoids-beta-carotene interference in order to promote the interruption or accumulation of the programmed biosynthesis of carotenoids. When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 microM fosmidomycin, carotenogenesis and the synthesis of beta-carotene were interrupted after two days in cultured D. salina cells. This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina. The effect at the level of proteins and RNA was not evident. Mevinolin treated D. salina cells exhibited carotenogenesis and beta-carotene levels very similar to those of control cell cultures indicating that mevinolin not pursued any indirect action in the biosynthesis of isoprenoids and had no effect at the level of the HMG-CoA reductase, the key enzyme of the Ac/MVA pathway.

Show MeSH
Supposed simplified pathway of the early steps of carotenogenesis and β-carotene accumulation in Dunaliella salina (modified after [21]).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2666888&req=5

f5-marinedrugs-07-00045: Supposed simplified pathway of the early steps of carotenogenesis and β-carotene accumulation in Dunaliella salina (modified after [21]).

Mentions: All experiments were performed in order to favor growth and to try to enhance the incorporation of carbon (from carbon dioxide) from air during photosynthesis. The photosynthetic autotrophic conditions promoted in cultures of Dunaliella leads to synthesis of pentose phosphate cycle substrates in the Calvin cycle from pyruvate pools, which conversion stimulate the apparent synthesis of the first precursors of the IPPP/DMAPP and lately incorporated into the chloroplast isoprenoids and carotenoids (Figure 5).


The carotenogenesis pathway via the isoprenoid-beta-carotene interference approach in a new strain of Dunaliella salina isolated from Baja California Mexico.

Paniagua-Michel J, Capa-Robles W, Olmos-Soto J, Gutierrez-Millan LE - Mar Drugs (2009)

Supposed simplified pathway of the early steps of carotenogenesis and β-carotene accumulation in Dunaliella salina (modified after [21]).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666888&req=5

f5-marinedrugs-07-00045: Supposed simplified pathway of the early steps of carotenogenesis and β-carotene accumulation in Dunaliella salina (modified after [21]).
Mentions: All experiments were performed in order to favor growth and to try to enhance the incorporation of carbon (from carbon dioxide) from air during photosynthesis. The photosynthetic autotrophic conditions promoted in cultures of Dunaliella leads to synthesis of pentose phosphate cycle substrates in the Calvin cycle from pyruvate pools, which conversion stimulate the apparent synthesis of the first precursors of the IPPP/DMAPP and lately incorporated into the chloroplast isoprenoids and carotenoids (Figure 5).

Bottom Line: When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 microM fosmidomycin, carotenogenesis and the synthesis of beta-carotene were interrupted after two days in cultured D. salina cells.This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina.The effect at the level of proteins and RNA was not evident.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biotechnology, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada 22860, México. jpaniagu@cicese.mx

ABSTRACT
D. salina is one of the recognized natural sources to produce beta-carotene, and an useful model for studying the role of inhibitors and enhancers of carotenogenesis. However there is little information in D. salina regarding whether the isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective inhibitors which in turn may further contribute to elucidate the early steps of carotenogenesis and biosynthesis of beta-carotene. In this study, Dunaliella salina (BC02) isolated from La Salina BC Mexico, was subjected to the method of isoprenoids-beta-carotene interference in order to promote the interruption or accumulation of the programmed biosynthesis of carotenoids. When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 microM fosmidomycin, carotenogenesis and the synthesis of beta-carotene were interrupted after two days in cultured D. salina cells. This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina. The effect at the level of proteins and RNA was not evident. Mevinolin treated D. salina cells exhibited carotenogenesis and beta-carotene levels very similar to those of control cell cultures indicating that mevinolin not pursued any indirect action in the biosynthesis of isoprenoids and had no effect at the level of the HMG-CoA reductase, the key enzyme of the Ac/MVA pathway.

Show MeSH