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Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

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Neighbor-joining tree based on nearly complete 16S rRNA gene sequences showing relationships between the 12 isolates active in the PIP1B assay and between them and the highest 16S rDNA sequence similar Micromonospora and Streptomyces species. Bifidobacterium bifidum DSM 20456 T (M38018) was used as the outgroup. Numbers at the nodes are percentage bootstrap values based on 1,000 replicates; only values 50% or above are given. Bar, 2% sequence divergence.
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f3-marinedrugs-07-00024: Neighbor-joining tree based on nearly complete 16S rRNA gene sequences showing relationships between the 12 isolates active in the PIP1B assay and between them and the highest 16S rDNA sequence similar Micromonospora and Streptomyces species. Bifidobacterium bifidum DSM 20456 T (M38018) was used as the outgroup. Numbers at the nodes are percentage bootstrap values based on 1,000 replicates; only values 50% or above are given. Bar, 2% sequence divergence.

Mentions: The 12 actinomycetes those metabolites active in the PTP1B screen were assigned to the genera Micromonospora and Streptomyces (Figure 3). Six out of the 8 Micromonospora strains formed relatively distinct phyletic lines though isolates 201806 and 215009 had identical 16S rRNA gene sequences to one another and to the type strain of M. chalcea. The 4 Streptomyces isolates were relatively closely related to the type strains of Streptomyces species. Indeed, isolate 172610 had an identical 16S rRNA gene sequence to the type strain of S. albus whereas isolate 216802 had a nearly identical sequence to S. violaceochromogenes NBRC 13100T.


Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Neighbor-joining tree based on nearly complete 16S rRNA gene sequences showing relationships between the 12 isolates active in the PIP1B assay and between them and the highest 16S rDNA sequence similar Micromonospora and Streptomyces species. Bifidobacterium bifidum DSM 20456 T (M38018) was used as the outgroup. Numbers at the nodes are percentage bootstrap values based on 1,000 replicates; only values 50% or above are given. Bar, 2% sequence divergence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666887&req=5

f3-marinedrugs-07-00024: Neighbor-joining tree based on nearly complete 16S rRNA gene sequences showing relationships between the 12 isolates active in the PIP1B assay and between them and the highest 16S rDNA sequence similar Micromonospora and Streptomyces species. Bifidobacterium bifidum DSM 20456 T (M38018) was used as the outgroup. Numbers at the nodes are percentage bootstrap values based on 1,000 replicates; only values 50% or above are given. Bar, 2% sequence divergence.
Mentions: The 12 actinomycetes those metabolites active in the PTP1B screen were assigned to the genera Micromonospora and Streptomyces (Figure 3). Six out of the 8 Micromonospora strains formed relatively distinct phyletic lines though isolates 201806 and 215009 had identical 16S rRNA gene sequences to one another and to the type strain of M. chalcea. The 4 Streptomyces isolates were relatively closely related to the type strains of Streptomyces species. Indeed, isolate 172610 had an identical 16S rRNA gene sequence to the type strain of S. albus whereas isolate 216802 had a nearly identical sequence to S. violaceochromogenes NBRC 13100T.

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

Show MeSH
Related in: MedlinePlus