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Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

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Related in: MedlinePlus

Neighbor-joining tree based on almost complete 16S rRNA gene sequences showing relationships between the 26 selected actinomycetes found to inhibit tumor cells in vitro, and between them and the type strains of the highest 16S rDNA sequence similarity. Numbers at the nodes indicate bootstrap values based on 1000 replicates; only values above 50% are shown. Bar, 1% sequence divergence.
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f2-marinedrugs-07-00024: Neighbor-joining tree based on almost complete 16S rRNA gene sequences showing relationships between the 26 selected actinomycetes found to inhibit tumor cells in vitro, and between them and the type strains of the highest 16S rDNA sequence similarity. Numbers at the nodes indicate bootstrap values based on 1000 replicates; only values above 50% are shown. Bar, 1% sequence divergence.

Mentions: The most taxonomically diverse actinomycetes were those showed activity against the tumor cells. It can be seen from Figure 2 that the 26 tested strains which gave positive results were assigned to 7 genera with just over half of them belonging to the genus Streptomyces. Most of the Streptomyces isolates formed distinct phyletic lines though strain 172621 was found to have an identical sequence to that of the type strain of S. coelicolor. Similarly, the 6 isolates belonging to the genus Micromonospora formed a diverse group, as did the 4 Nocardia isolates. It was interesting that the single isolate assigned to the genus Actinomadura had an identical 16S rRNA gene sequence to the type strain of A. glauciflava. In contrast, the single isolates belonging to the genera Nonomuraea, Rhodococcus, and Verrucosispora formed relatively distinct phyletic lines.


Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Neighbor-joining tree based on almost complete 16S rRNA gene sequences showing relationships between the 26 selected actinomycetes found to inhibit tumor cells in vitro, and between them and the type strains of the highest 16S rDNA sequence similarity. Numbers at the nodes indicate bootstrap values based on 1000 replicates; only values above 50% are shown. Bar, 1% sequence divergence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666887&req=5

f2-marinedrugs-07-00024: Neighbor-joining tree based on almost complete 16S rRNA gene sequences showing relationships between the 26 selected actinomycetes found to inhibit tumor cells in vitro, and between them and the type strains of the highest 16S rDNA sequence similarity. Numbers at the nodes indicate bootstrap values based on 1000 replicates; only values above 50% are shown. Bar, 1% sequence divergence.
Mentions: The most taxonomically diverse actinomycetes were those showed activity against the tumor cells. It can be seen from Figure 2 that the 26 tested strains which gave positive results were assigned to 7 genera with just over half of them belonging to the genus Streptomyces. Most of the Streptomyces isolates formed distinct phyletic lines though strain 172621 was found to have an identical sequence to that of the type strain of S. coelicolor. Similarly, the 6 isolates belonging to the genus Micromonospora formed a diverse group, as did the 4 Nocardia isolates. It was interesting that the single isolate assigned to the genus Actinomadura had an identical 16S rRNA gene sequence to the type strain of A. glauciflava. In contrast, the single isolates belonging to the genera Nonomuraea, Rhodococcus, and Verrucosispora formed relatively distinct phyletic lines.

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

Show MeSH
Related in: MedlinePlus