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Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

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Related in: MedlinePlus

Percentage of bioactive strains isolated from composite soil samples collected from the mangrove sites. The percentages indicate the number of positive hits against each of 4 targets over the total number of strains isolated at each site.
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f1-marinedrugs-07-00024: Percentage of bioactive strains isolated from composite soil samples collected from the mangrove sites. The percentages indicate the number of positive hits against each of 4 targets over the total number of strains isolated at each site.

Mentions: The highest percentage of anti-tumor and anti- C. albicans activity was found with strains isolated from samples collected from Zhanjiang. Isolates from the Wenchang samples showed activity against all of the screening models, including two out of the three isolates inhibit on caspase 3, possibly reflecting the diverse nature of the plant species growing at these well preserved locations (Figure 1). In contrast, the lowest number of bioactive strains was recorded from the Guangxi samples; it was noted that the soil from this location contained more sand and less organic matter than those from other sites.


Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China.

Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS - Mar Drugs (2009)

Percentage of bioactive strains isolated from composite soil samples collected from the mangrove sites. The percentages indicate the number of positive hits against each of 4 targets over the total number of strains isolated at each site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666887&req=5

f1-marinedrugs-07-00024: Percentage of bioactive strains isolated from composite soil samples collected from the mangrove sites. The percentages indicate the number of positive hits against each of 4 targets over the total number of strains isolated at each site.
Mentions: The highest percentage of anti-tumor and anti- C. albicans activity was found with strains isolated from samples collected from Zhanjiang. Isolates from the Wenchang samples showed activity against all of the screening models, including two out of the three isolates inhibit on caspase 3, possibly reflecting the diverse nature of the plant species growing at these well preserved locations (Figure 1). In contrast, the lowest number of bioactive strains was recorded from the Guangxi samples; it was noted that the soil from this location contained more sand and less organic matter than those from other sites.

Bottom Line: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites.Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces.Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, PR China. k1022@163.net

ABSTRACT
The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.

Show MeSH
Related in: MedlinePlus