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Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamine.

Zhong JP, Wang G, Shang JH, Pan JQ, Li K, Huang Y, Liu HZ - Mar Drugs (2009)

Bottom Line: At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells.Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05).In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals.

View Article: PubMed Central - PubMed

Affiliation: Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, PR China.

ABSTRACT
To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.

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Related in: MedlinePlus

The effects of squid ink on antioxidant ability of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. &P<0.05, or &&P<0.01, vs the value of control mice (group A). *P<0.05, or **P<0.01, vs the value of control mice (group A). #P<0.05, or ##P<0.01, vs the value of model mice (group C). Group B represents positive control group.
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f3-marinedrugs-07-00009: The effects of squid ink on antioxidant ability of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. &P<0.05, or &&P<0.01, vs the value of control mice (group A). *P<0.05, or **P<0.01, vs the value of control mice (group A). #P<0.05, or ##P<0.01, vs the value of model mice (group C). Group B represents positive control group.

Mentions: Data (Figure 3) indicated that four parameters of spleen antioxidant ability were all modified markedly by cyclophosphamine (P<0.05 or P<0.01), whereas, the changes were all reversed by squid ink extract and low dosage squid ink extract redressed significantly the alterations induced by the chemotherapeutics (P<0.01). Effects of the high dosage of marine drug on the changes were also observed, but the results were diverse, as the medicine changed significantly activities of CAT and GSH-Px (P<0.01) as well as content of MDA (P<0.05), and did not alter the activity of SOD (P>0.05). In addition, this experiment revealed that squid ink extract changed the antioxidant ability of the spleen in mice, as the SOD and GSH-Px activities in the spleens from the positive control mice were higher than those in spleens from control mice (P<0.05 or P<0.01), and the spleen MDA content in group B was downregulated by sepia extract (P<0.01).


Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamine.

Zhong JP, Wang G, Shang JH, Pan JQ, Li K, Huang Y, Liu HZ - Mar Drugs (2009)

The effects of squid ink on antioxidant ability of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. &P<0.05, or &&P<0.01, vs the value of control mice (group A). *P<0.05, or **P<0.01, vs the value of control mice (group A). #P<0.05, or ##P<0.01, vs the value of model mice (group C). Group B represents positive control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666885&req=5

f3-marinedrugs-07-00009: The effects of squid ink on antioxidant ability of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. &P<0.05, or &&P<0.01, vs the value of control mice (group A). *P<0.05, or **P<0.01, vs the value of control mice (group A). #P<0.05, or ##P<0.01, vs the value of model mice (group C). Group B represents positive control group.
Mentions: Data (Figure 3) indicated that four parameters of spleen antioxidant ability were all modified markedly by cyclophosphamine (P<0.05 or P<0.01), whereas, the changes were all reversed by squid ink extract and low dosage squid ink extract redressed significantly the alterations induced by the chemotherapeutics (P<0.01). Effects of the high dosage of marine drug on the changes were also observed, but the results were diverse, as the medicine changed significantly activities of CAT and GSH-Px (P<0.01) as well as content of MDA (P<0.05), and did not alter the activity of SOD (P>0.05). In addition, this experiment revealed that squid ink extract changed the antioxidant ability of the spleen in mice, as the SOD and GSH-Px activities in the spleens from the positive control mice were higher than those in spleens from control mice (P<0.05 or P<0.01), and the spleen MDA content in group B was downregulated by sepia extract (P<0.01).

Bottom Line: At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells.Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05).In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals.

View Article: PubMed Central - PubMed

Affiliation: Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, PR China.

ABSTRACT
To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.

Show MeSH
Related in: MedlinePlus