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Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamine.

Zhong JP, Wang G, Shang JH, Pan JQ, Li K, Huang Y, Liu HZ - Mar Drugs (2009)

Bottom Line: At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells.Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05).In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals.

View Article: PubMed Central - PubMed

Affiliation: Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, PR China.

ABSTRACT
To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.

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Related in: MedlinePlus

The effects of squid ink on organ index of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. * indicates that there was a significant difference (P<0.05) between model mice (group C) and control mice (group A), ## indicates a difference (P<0.01) between low-dose group (group D) and model mice (group C), # indicates a difference (P<0.05) between high-dose group (group E) and model mice. Group B represents positive control group.
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f2-marinedrugs-07-00009: The effects of squid ink on organ index of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. * indicates that there was a significant difference (P<0.05) between model mice (group C) and control mice (group A), ## indicates a difference (P<0.01) between low-dose group (group D) and model mice (group C), # indicates a difference (P<0.05) between high-dose group (group E) and model mice. Group B represents positive control group.

Mentions: In Figure 2 it is shown that the organs in the model mice were affected by cyclophosphamine, and the organ indexes of their spleens were significantly synchronously downregulated (P<0.05) by the chemotherapeutic treatment. The figure shows that the parameters in the experimental mice (groups D and E) showed an obvious elevation (P<0.01 or P<0.05) compared to model group mice.


Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamine.

Zhong JP, Wang G, Shang JH, Pan JQ, Li K, Huang Y, Liu HZ - Mar Drugs (2009)

The effects of squid ink on organ index of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. * indicates that there was a significant difference (P<0.05) between model mice (group C) and control mice (group A), ## indicates a difference (P<0.01) between low-dose group (group D) and model mice (group C), # indicates a difference (P<0.05) between high-dose group (group E) and model mice. Group B represents positive control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2666885&req=5

f2-marinedrugs-07-00009: The effects of squid ink on organ index of spleen in mice induced by cyclophosphamine. Bars indicate means±standard deviations. * indicates that there was a significant difference (P<0.05) between model mice (group C) and control mice (group A), ## indicates a difference (P<0.01) between low-dose group (group D) and model mice (group C), # indicates a difference (P<0.05) between high-dose group (group E) and model mice. Group B represents positive control group.
Mentions: In Figure 2 it is shown that the organs in the model mice were affected by cyclophosphamine, and the organ indexes of their spleens were significantly synchronously downregulated (P<0.05) by the chemotherapeutic treatment. The figure shows that the parameters in the experimental mice (groups D and E) showed an obvious elevation (P<0.01 or P<0.05) compared to model group mice.

Bottom Line: At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells.Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05).In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals.

View Article: PubMed Central - PubMed

Affiliation: Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, PR China.

ABSTRACT
To investigate the protective effects of squid ink in chemotherapy, BALB/c mice were used as animal models of injuries induced by cyclophosphamine, a well known chemotherapeutic drug. The mice were randomly divided into five groups with the same number of males and females in each group. At the end of the experiment, animals were sacrificed to investigate organ indexes and antioxidant ability of the spleen, peripheral blood profile and quantities of bone marrow nucleated cells. Results showed that the hemopoietic function of mice was injured by cyclophosphamine, as indicated by decreases of contents of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), while platelets were not affected (P>0.05), as well as modification of organ indexes (P<0.05) and spleen antioxidant ability (P<0.05 or P<0.01), whereas sepia extract markedly increased the levels of erythrocytes, leukocytes, hemoglobin and bone marrow nucleated cells (P<0.01), but not platelets (P>0.05), and reversed the effects of cyclophosphamine on organ indexes and antioxidant ability of spleen (P<0.01 or P<0.05). In addition, squid ink extract did not change marrow hemopoiesis but improved the antioxidant ability of spleen in the animals. The data suggest that squid ink extract can protect the hemopoietic system from chemotherapeutic injury and could be employed to develop cell-protective drugs for use in clinical treatment of tumours.

Show MeSH
Related in: MedlinePlus