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Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.

Unkrüer B, Pekcec A, Fuest C, Wehmeyer A, Balda MS, Horn A, Baumgärtner W, Potschka H - BMC Neurosci (2009)

Bottom Line: The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans.In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans.Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany. berni-unkrueer@web.de

ABSTRACT

Background: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.

Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.

Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

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Analysis of neural YB-1 expression in the hippocampal hilus. Data are given as neural density (mean ± SEM). Significant differences to controls are indicated by an asterisk. One way analysis of variance indicated that groups differ significantly (p = 0.0044). (A) Analysis of the hilar density of YB-1 reactive neurons two (n = 2), four (n = 6) or eight hours (n = 7) after status epilepticus did not show significant differences to controls (n = 8) (in all cases p > 0.05). In contrast, the neural density of YB-1 expressing cells was decreased 48 hours (n = 6) after status epilepticus (p = 0.0017). (B) Representative image of the dentate hilus (H) of a control rat. (C) Representative image of the dentate hilus (H) of a rat 48 hours after status epilepticus. Note the tremendous decrease in YB-1 expressing hilar neurons. (DG = dentate gyrus). Scale bar = 100 μm.
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Figure 3: Analysis of neural YB-1 expression in the hippocampal hilus. Data are given as neural density (mean ± SEM). Significant differences to controls are indicated by an asterisk. One way analysis of variance indicated that groups differ significantly (p = 0.0044). (A) Analysis of the hilar density of YB-1 reactive neurons two (n = 2), four (n = 6) or eight hours (n = 7) after status epilepticus did not show significant differences to controls (n = 8) (in all cases p > 0.05). In contrast, the neural density of YB-1 expressing cells was decreased 48 hours (n = 6) after status epilepticus (p = 0.0017). (B) Representative image of the dentate hilus (H) of a control rat. (C) Representative image of the dentate hilus (H) of a rat 48 hours after status epilepticus. Note the tremendous decrease in YB-1 expressing hilar neurons. (DG = dentate gyrus). Scale bar = 100 μm.

Mentions: The overall expression pattern of YB-1 in hippocampal cells with a neuronal morphology did not change during the first 48 h following SE. No induction was observed in hippocampal cells at the different time points investigated (Fig. 3). The significant reduction 48 h following SE is likely to be related to hippocampal cell loss that is known to be evident at this time point (Fig. 3).


Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.

Unkrüer B, Pekcec A, Fuest C, Wehmeyer A, Balda MS, Horn A, Baumgärtner W, Potschka H - BMC Neurosci (2009)

Analysis of neural YB-1 expression in the hippocampal hilus. Data are given as neural density (mean ± SEM). Significant differences to controls are indicated by an asterisk. One way analysis of variance indicated that groups differ significantly (p = 0.0044). (A) Analysis of the hilar density of YB-1 reactive neurons two (n = 2), four (n = 6) or eight hours (n = 7) after status epilepticus did not show significant differences to controls (n = 8) (in all cases p > 0.05). In contrast, the neural density of YB-1 expressing cells was decreased 48 hours (n = 6) after status epilepticus (p = 0.0017). (B) Representative image of the dentate hilus (H) of a control rat. (C) Representative image of the dentate hilus (H) of a rat 48 hours after status epilepticus. Note the tremendous decrease in YB-1 expressing hilar neurons. (DG = dentate gyrus). Scale bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2666744&req=5

Figure 3: Analysis of neural YB-1 expression in the hippocampal hilus. Data are given as neural density (mean ± SEM). Significant differences to controls are indicated by an asterisk. One way analysis of variance indicated that groups differ significantly (p = 0.0044). (A) Analysis of the hilar density of YB-1 reactive neurons two (n = 2), four (n = 6) or eight hours (n = 7) after status epilepticus did not show significant differences to controls (n = 8) (in all cases p > 0.05). In contrast, the neural density of YB-1 expressing cells was decreased 48 hours (n = 6) after status epilepticus (p = 0.0017). (B) Representative image of the dentate hilus (H) of a control rat. (C) Representative image of the dentate hilus (H) of a rat 48 hours after status epilepticus. Note the tremendous decrease in YB-1 expressing hilar neurons. (DG = dentate gyrus). Scale bar = 100 μm.
Mentions: The overall expression pattern of YB-1 in hippocampal cells with a neuronal morphology did not change during the first 48 h following SE. No induction was observed in hippocampal cells at the different time points investigated (Fig. 3). The significant reduction 48 h following SE is likely to be related to hippocampal cell loss that is known to be evident at this time point (Fig. 3).

Bottom Line: The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans.In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans.Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany. berni-unkrueer@web.de

ABSTRACT

Background: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.

Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.

Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

Show MeSH
Related in: MedlinePlus