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Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.

Unkrüer B, Pekcec A, Fuest C, Wehmeyer A, Balda MS, Horn A, Baumgärtner W, Potschka H - BMC Neurosci (2009)

Bottom Line: The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans.In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans.Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany. berni-unkrueer@web.de

ABSTRACT

Background: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.

Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.

Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

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Micrographs showing expression of the transcription factor YB-1 in the rat, macaque and human brain (A-H). (A) Image displaying hippocampal YB-1 expression visualized by a DAB-staining method in a 14 μm thin section of an adult rat's hippocampus (DG = dentate gyrus, H = hilus of the dentate gyrus, CA = cornu ammonis formation). (B) Representative example of YB-1 expression in the piriform cortex (layer I-III) of an adult rat. Note, that the expression of YB-1 is predominantly related to cells in the layer III of the piriform cortex. (C) Image showing YB-1 expressing granule cells of the dentate gyrus and YB-1 expressing cells in the hilar (H) subregion of the hippocampus of an adult rat. (D) Image displaying YB-1 expression visualized by a DAB-staining method in a 14 μm thick section of the parahippocampal cortex (PHC) of a rat. YB-1 expression appears throughout all layers of the cortex. (E) Image displaying YB-1 expression visualized by a DAB-staining method in a 40 μm thick section of a macaque's parahippocampal cortex (PHC). In this image YB-1 appears to be stronger expressed as in the rat's brain due to the relative thickness of this section in comparison to the thinner sections of the rat. (F) Image displaying YB-1 expression visualized by a DAB-staining method in a 3 μm thin section of a human parahippocampal cortex (PHC). Due to the section thickness of only 3 μm, YB-1 appears to be less expressed compared to images of the rat's and macaque's brain. (G) Image displaying YB-1 expressing granule cells of the dentate gyrus (DG) as well as YB-1 expressing cells in the hilar (H) subregion and the molecular layer (Mol) of the hippocampal formation in the brain of a macaque. (H) YB-1 expression in the hippocampal CA-1 region of the human brain. A: scale bar = 100 μm. B-H: scale bar = 20 μm.
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Figure 1: Micrographs showing expression of the transcription factor YB-1 in the rat, macaque and human brain (A-H). (A) Image displaying hippocampal YB-1 expression visualized by a DAB-staining method in a 14 μm thin section of an adult rat's hippocampus (DG = dentate gyrus, H = hilus of the dentate gyrus, CA = cornu ammonis formation). (B) Representative example of YB-1 expression in the piriform cortex (layer I-III) of an adult rat. Note, that the expression of YB-1 is predominantly related to cells in the layer III of the piriform cortex. (C) Image showing YB-1 expressing granule cells of the dentate gyrus and YB-1 expressing cells in the hilar (H) subregion of the hippocampus of an adult rat. (D) Image displaying YB-1 expression visualized by a DAB-staining method in a 14 μm thick section of the parahippocampal cortex (PHC) of a rat. YB-1 expression appears throughout all layers of the cortex. (E) Image displaying YB-1 expression visualized by a DAB-staining method in a 40 μm thick section of a macaque's parahippocampal cortex (PHC). In this image YB-1 appears to be stronger expressed as in the rat's brain due to the relative thickness of this section in comparison to the thinner sections of the rat. (F) Image displaying YB-1 expression visualized by a DAB-staining method in a 3 μm thin section of a human parahippocampal cortex (PHC). Due to the section thickness of only 3 μm, YB-1 appears to be less expressed compared to images of the rat's and macaque's brain. (G) Image displaying YB-1 expressing granule cells of the dentate gyrus (DG) as well as YB-1 expressing cells in the hilar (H) subregion and the molecular layer (Mol) of the hippocampal formation in the brain of a macaque. (H) YB-1 expression in the hippocampal CA-1 region of the human brain. A: scale bar = 100 μm. B-H: scale bar = 20 μm.

Mentions: Analysis of YB-1 immunolabelling in rat brain tissue revealed a strong expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex, as well as throughout all layers of the parahippocampal cortex (Fig. 1A–D). Based on the morphology the vast majority of labelled cells presented a neuronal phenotype. An identical expression pattern was observed in the hippocampus and the parahippocampal cortex of macaques and humans (Fig. 1E–H). In the hilus of the hippocampus of all three species single YB-1 positive neurons were evident. Expression of YB-1 in neurons was substantiated by double-labelling with the neuronal cell marker NeuN. In rat and macaque tissue representative YB-1 immunoreactive cells with a neuronal morphology were carefully analyzed on serial optical planes. This analysis confirmed a co-localization of YB-1 and NeuN (Fig. 2A–B). The majority of cells showed a dominant YB-1 expression in the cytoplasm, whereas a predominant nuclear localization of YB-1 was obvious in some cells only (Fig. 2A–B). In contrast, there was no evidence for a co-localization of YB-1 with the astroglial cell marker GFAP in different regions of the rat brain (Fig. 2C). Thereby these data imply that YB-1 is not expressed in astrocytes in the naïve brain to a relevant extent.


Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.

Unkrüer B, Pekcec A, Fuest C, Wehmeyer A, Balda MS, Horn A, Baumgärtner W, Potschka H - BMC Neurosci (2009)

Micrographs showing expression of the transcription factor YB-1 in the rat, macaque and human brain (A-H). (A) Image displaying hippocampal YB-1 expression visualized by a DAB-staining method in a 14 μm thin section of an adult rat's hippocampus (DG = dentate gyrus, H = hilus of the dentate gyrus, CA = cornu ammonis formation). (B) Representative example of YB-1 expression in the piriform cortex (layer I-III) of an adult rat. Note, that the expression of YB-1 is predominantly related to cells in the layer III of the piriform cortex. (C) Image showing YB-1 expressing granule cells of the dentate gyrus and YB-1 expressing cells in the hilar (H) subregion of the hippocampus of an adult rat. (D) Image displaying YB-1 expression visualized by a DAB-staining method in a 14 μm thick section of the parahippocampal cortex (PHC) of a rat. YB-1 expression appears throughout all layers of the cortex. (E) Image displaying YB-1 expression visualized by a DAB-staining method in a 40 μm thick section of a macaque's parahippocampal cortex (PHC). In this image YB-1 appears to be stronger expressed as in the rat's brain due to the relative thickness of this section in comparison to the thinner sections of the rat. (F) Image displaying YB-1 expression visualized by a DAB-staining method in a 3 μm thin section of a human parahippocampal cortex (PHC). Due to the section thickness of only 3 μm, YB-1 appears to be less expressed compared to images of the rat's and macaque's brain. (G) Image displaying YB-1 expressing granule cells of the dentate gyrus (DG) as well as YB-1 expressing cells in the hilar (H) subregion and the molecular layer (Mol) of the hippocampal formation in the brain of a macaque. (H) YB-1 expression in the hippocampal CA-1 region of the human brain. A: scale bar = 100 μm. B-H: scale bar = 20 μm.
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Related In: Results  -  Collection

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Figure 1: Micrographs showing expression of the transcription factor YB-1 in the rat, macaque and human brain (A-H). (A) Image displaying hippocampal YB-1 expression visualized by a DAB-staining method in a 14 μm thin section of an adult rat's hippocampus (DG = dentate gyrus, H = hilus of the dentate gyrus, CA = cornu ammonis formation). (B) Representative example of YB-1 expression in the piriform cortex (layer I-III) of an adult rat. Note, that the expression of YB-1 is predominantly related to cells in the layer III of the piriform cortex. (C) Image showing YB-1 expressing granule cells of the dentate gyrus and YB-1 expressing cells in the hilar (H) subregion of the hippocampus of an adult rat. (D) Image displaying YB-1 expression visualized by a DAB-staining method in a 14 μm thick section of the parahippocampal cortex (PHC) of a rat. YB-1 expression appears throughout all layers of the cortex. (E) Image displaying YB-1 expression visualized by a DAB-staining method in a 40 μm thick section of a macaque's parahippocampal cortex (PHC). In this image YB-1 appears to be stronger expressed as in the rat's brain due to the relative thickness of this section in comparison to the thinner sections of the rat. (F) Image displaying YB-1 expression visualized by a DAB-staining method in a 3 μm thin section of a human parahippocampal cortex (PHC). Due to the section thickness of only 3 μm, YB-1 appears to be less expressed compared to images of the rat's and macaque's brain. (G) Image displaying YB-1 expressing granule cells of the dentate gyrus (DG) as well as YB-1 expressing cells in the hilar (H) subregion and the molecular layer (Mol) of the hippocampal formation in the brain of a macaque. (H) YB-1 expression in the hippocampal CA-1 region of the human brain. A: scale bar = 100 μm. B-H: scale bar = 20 μm.
Mentions: Analysis of YB-1 immunolabelling in rat brain tissue revealed a strong expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex, as well as throughout all layers of the parahippocampal cortex (Fig. 1A–D). Based on the morphology the vast majority of labelled cells presented a neuronal phenotype. An identical expression pattern was observed in the hippocampus and the parahippocampal cortex of macaques and humans (Fig. 1E–H). In the hilus of the hippocampus of all three species single YB-1 positive neurons were evident. Expression of YB-1 in neurons was substantiated by double-labelling with the neuronal cell marker NeuN. In rat and macaque tissue representative YB-1 immunoreactive cells with a neuronal morphology were carefully analyzed on serial optical planes. This analysis confirmed a co-localization of YB-1 and NeuN (Fig. 2A–B). The majority of cells showed a dominant YB-1 expression in the cytoplasm, whereas a predominant nuclear localization of YB-1 was obvious in some cells only (Fig. 2A–B). In contrast, there was no evidence for a co-localization of YB-1 with the astroglial cell marker GFAP in different regions of the rat brain (Fig. 2C). Thereby these data imply that YB-1 is not expressed in astrocytes in the naïve brain to a relevant extent.

Bottom Line: The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans.In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans.Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany. berni-unkrueer@web.de

ABSTRACT

Background: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.

Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.

Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

Show MeSH
Related in: MedlinePlus