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The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats.

Budin SB, Othman F, Louis SR, Bakar MA, Das S, Mohamed J - Clinics (Sao Paulo) (2009)

Bottom Line: Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions.Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Allied Health Sciences, Universiti Kebangsaan, Malaysia.

ABSTRACT

Objective: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats.

Methods: Animals were divided into three groups: (i) normal non-diabetic (NDM), (ii) diabetic treated (tocotrienol-rich fractions - TRF) and (iii) diabetic untreated (non-TRF). The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight) daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated.

Results: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE) and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.

Conclusion: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

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Related in: MedlinePlus

Electron microscopic findings on the aorta of the diabetic rats following eight weeks of study. (A) - (Original magnification × 4400) Proliferation and fragmentation of medial smooth muscle cells (SMC) in both the intima and media layers. The extracellular matrix also consists predominantly of amorphous material (AM), as apparent in the media of diabetic rats. Endothelial cells (EC) lost their squamous characteristics and became atrophic. (B) - (Original magnification × 4400) A smooth muscle cell (SMC) is migrating from the media into the intima, breaking through the elastic lamina (EL). (C) - (Original magnification × 6500) A mononuclear cell (MC) and fragmented smooth muscle cell (SMC) at the subendothelial layer of the intima. We observed fragmentation and reduplication of elastic lamina
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f4-15-br-0219: Electron microscopic findings on the aorta of the diabetic rats following eight weeks of study. (A) - (Original magnification × 4400) Proliferation and fragmentation of medial smooth muscle cells (SMC) in both the intima and media layers. The extracellular matrix also consists predominantly of amorphous material (AM), as apparent in the media of diabetic rats. Endothelial cells (EC) lost their squamous characteristics and became atrophic. (B) - (Original magnification × 4400) A smooth muscle cell (SMC) is migrating from the media into the intima, breaking through the elastic lamina (EL). (C) - (Original magnification × 6500) A mononuclear cell (MC) and fragmented smooth muscle cell (SMC) at the subendothelial layer of the intima. We observed fragmentation and reduplication of elastic lamina

Mentions: STZ-induced diabetes caused severe alterations in the structure of the vascular wall in non-TRF rats, as shown in Fig. 4A, 4B and 4C. The tunica intima of the aorta was irregular and the endothelial cells showed irregular distributions with atrophic characteristics. The subendothelial region was thickened in patches; infiltrating mononuclear cells were observed. Aortic media showed irregular VSMCs between partly fragmented elastic lamina within degenerative interstitial matrix. The elastic lamina also appeared fragmented and reduplicated. Irregularly fragmented and atrophic smooth muscle cells were frequently observed together with excess extracellular matrix, predominantly consisting of electron-dense amorphous material. There was a migration of VSMC from the tunica media into the tunica intima, with a break in the elastic lamina.


The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats.

Budin SB, Othman F, Louis SR, Bakar MA, Das S, Mohamed J - Clinics (Sao Paulo) (2009)

Electron microscopic findings on the aorta of the diabetic rats following eight weeks of study. (A) - (Original magnification × 4400) Proliferation and fragmentation of medial smooth muscle cells (SMC) in both the intima and media layers. The extracellular matrix also consists predominantly of amorphous material (AM), as apparent in the media of diabetic rats. Endothelial cells (EC) lost their squamous characteristics and became atrophic. (B) - (Original magnification × 4400) A smooth muscle cell (SMC) is migrating from the media into the intima, breaking through the elastic lamina (EL). (C) - (Original magnification × 6500) A mononuclear cell (MC) and fragmented smooth muscle cell (SMC) at the subendothelial layer of the intima. We observed fragmentation and reduplication of elastic lamina
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2666447&req=5

f4-15-br-0219: Electron microscopic findings on the aorta of the diabetic rats following eight weeks of study. (A) - (Original magnification × 4400) Proliferation and fragmentation of medial smooth muscle cells (SMC) in both the intima and media layers. The extracellular matrix also consists predominantly of amorphous material (AM), as apparent in the media of diabetic rats. Endothelial cells (EC) lost their squamous characteristics and became atrophic. (B) - (Original magnification × 4400) A smooth muscle cell (SMC) is migrating from the media into the intima, breaking through the elastic lamina (EL). (C) - (Original magnification × 6500) A mononuclear cell (MC) and fragmented smooth muscle cell (SMC) at the subendothelial layer of the intima. We observed fragmentation and reduplication of elastic lamina
Mentions: STZ-induced diabetes caused severe alterations in the structure of the vascular wall in non-TRF rats, as shown in Fig. 4A, 4B and 4C. The tunica intima of the aorta was irregular and the endothelial cells showed irregular distributions with atrophic characteristics. The subendothelial region was thickened in patches; infiltrating mononuclear cells were observed. Aortic media showed irregular VSMCs between partly fragmented elastic lamina within degenerative interstitial matrix. The elastic lamina also appeared fragmented and reduplicated. Irregularly fragmented and atrophic smooth muscle cells were frequently observed together with excess extracellular matrix, predominantly consisting of electron-dense amorphous material. There was a migration of VSMC from the tunica media into the tunica intima, with a break in the elastic lamina.

Bottom Line: Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions.Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Allied Health Sciences, Universiti Kebangsaan, Malaysia.

ABSTRACT

Objective: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats.

Methods: Animals were divided into three groups: (i) normal non-diabetic (NDM), (ii) diabetic treated (tocotrienol-rich fractions - TRF) and (iii) diabetic untreated (non-TRF). The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight) daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated.

Results: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE) and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.

Conclusion: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

Show MeSH
Related in: MedlinePlus