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The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats.

Budin SB, Othman F, Louis SR, Bakar MA, Das S, Mohamed J - Clinics (Sao Paulo) (2009)

Bottom Line: Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions.Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Allied Health Sciences, Universiti Kebangsaan, Malaysia.

ABSTRACT

Objective: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats.

Methods: Animals were divided into three groups: (i) normal non-diabetic (NDM), (ii) diabetic treated (tocotrienol-rich fractions - TRF) and (iii) diabetic untreated (non-TRF). The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight) daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated.

Results: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE) and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.

Conclusion: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

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Electron microscopic findings on the aorta of control rats at eight weeks of study. (A) (Original magnification × 4400) The media layer is composed mainly of smooth muscle cells (SMC) with normal appearance, elastic lamina (EL) and extracellular matrix (EM) between the elastic lamina and smooth muscle cells. (B) (Original magnification × 11000) Extracellular matrix contains scattered collagen fibers (CF) and the endothelial cells appear smooth
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f3-15-br-0219: Electron microscopic findings on the aorta of control rats at eight weeks of study. (A) (Original magnification × 4400) The media layer is composed mainly of smooth muscle cells (SMC) with normal appearance, elastic lamina (EL) and extracellular matrix (EM) between the elastic lamina and smooth muscle cells. (B) (Original magnification × 11000) Extracellular matrix contains scattered collagen fibers (CF) and the endothelial cells appear smooth

Mentions: Under electron microscopic examination, the aortas from the control rats were observed to have smooth muscle cells, internal elastic lamina and a small amount of extracellular matrix between the elastic lamina and vascular smooth muscle cells (VSMC) (Figure 3A). The aortic tunica media also showed a regular appearance, including smooth muscle cells, between the elastic lamina and within a homogenous interstitial matrix. Endothelial cells in the aortas from the control rats demonstrated a characteristic smooth and uniform phenotype (Figure 3B).


The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats.

Budin SB, Othman F, Louis SR, Bakar MA, Das S, Mohamed J - Clinics (Sao Paulo) (2009)

Electron microscopic findings on the aorta of control rats at eight weeks of study. (A) (Original magnification × 4400) The media layer is composed mainly of smooth muscle cells (SMC) with normal appearance, elastic lamina (EL) and extracellular matrix (EM) between the elastic lamina and smooth muscle cells. (B) (Original magnification × 11000) Extracellular matrix contains scattered collagen fibers (CF) and the endothelial cells appear smooth
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2666447&req=5

f3-15-br-0219: Electron microscopic findings on the aorta of control rats at eight weeks of study. (A) (Original magnification × 4400) The media layer is composed mainly of smooth muscle cells (SMC) with normal appearance, elastic lamina (EL) and extracellular matrix (EM) between the elastic lamina and smooth muscle cells. (B) (Original magnification × 11000) Extracellular matrix contains scattered collagen fibers (CF) and the endothelial cells appear smooth
Mentions: Under electron microscopic examination, the aortas from the control rats were observed to have smooth muscle cells, internal elastic lamina and a small amount of extracellular matrix between the elastic lamina and vascular smooth muscle cells (VSMC) (Figure 3A). The aortic tunica media also showed a regular appearance, including smooth muscle cells, between the elastic lamina and within a homogenous interstitial matrix. Endothelial cells in the aortas from the control rats demonstrated a characteristic smooth and uniform phenotype (Figure 3B).

Bottom Line: Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions.Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Science, Faculty of Allied Health Sciences, Universiti Kebangsaan, Malaysia.

ABSTRACT

Objective: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats.

Methods: Animals were divided into three groups: (i) normal non-diabetic (NDM), (ii) diabetic treated (tocotrienol-rich fractions - TRF) and (iii) diabetic untreated (non-TRF). The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight) daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated.

Results: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE) and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall.

Conclusion: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

Show MeSH
Related in: MedlinePlus