Mentions: We will review a new chip design with a high-capacityenrichment column specifically developed for the analysis of a wide polarityrange of pharmaceutical small molecules. Retention time precision was betterthan 0.2% RSD on an Agilent 6410 QQQ HPLC chip system (see Figure 12). Typical pharmaceuticalcompounds were detected with sensitivities in the lower fg range absolute oncolumn. Linearity for quantitation was achieved over a wide concentration range.Both hydrophilic and hydrophobic analytes in a log P range from 0 to 4 werereproducibly enriched on the new small molecule chip. To demonstrate chipperformance for DMPK samples, imipramine was analyzed out of human serum.Despite the complex biological matrices, low fg levels were detected.
Affiliation: P S Analytical, Arthur House, Crayfields Industrial Estate, Main Road, Orpington, Kent BR5 3Hp, UK.
No MeSH data available.