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Abstracts of papers presented at the 2008 pittsburgh conference.

Stockwell PB - J Autom Methods Manag Chem (2009)

View Article: PubMed Central - PubMed

Affiliation: P S Analytical, Arthur House, Crayfields Industrial Estate, Main Road, Orpington, Kent BR5 3Hp, UK.

No MeSH data available.


Measurements of glycerol-dissolved ovalbumin adsorption by using QCM. ΔF2; adsorbed amount of ovalbumin only, Δ(F1 − F2)/2; viscous load of glycerol, ΔFs; adsorbed amount of ovalbumin + viscous load of glycerol.
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fig7: Measurements of glycerol-dissolved ovalbumin adsorption by using QCM. ΔF2; adsorbed amount of ovalbumin only, Δ(F1 − F2)/2; viscous load of glycerol, ΔFs; adsorbed amount of ovalbumin + viscous load of glycerol.

Mentions: The piezoelectric quartz crystal microbalance (QCM) is ahighly sensitive mass-measuring device on a surface, and has been used as abiosensor in aqueous solutions with its properties of nonlabel and real-timemonitoring. Applications of QCM instruments cover wide range of research fieldssuch as protein-protein interaction, protein-DNA interaction,material-biomolecule interaction, and material evaluations. In some cases (e.g.,protein samples dissolved in highly viscous solution such as glycerol), viscousloading significantly affected signal of mass load of protein adsorption on aQCM. Recently, we succeeded to develop the separate monitoring system of massload and viscous load by using frequency F2 measurement of QCM. In this study,we demonstrated some applications by using this system, such as viscoussolution-dissolved protein adsorption, ethanol-dissolved molecule adsorption,cation-dependent DNA conformation change, and temperature-dependentconformation change of polymer materials. We could analyze these reactions thatwere complicated by using conventional QCM system; these results can expand thepossibility of QCM instruments as biosensors (see Figure 7).


Abstracts of papers presented at the 2008 pittsburgh conference.

Stockwell PB - J Autom Methods Manag Chem (2009)

Measurements of glycerol-dissolved ovalbumin adsorption by using QCM. ΔF2; adsorbed amount of ovalbumin only, Δ(F1 − F2)/2; viscous load of glycerol, ΔFs; adsorbed amount of ovalbumin + viscous load of glycerol.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2665694&req=5

fig7: Measurements of glycerol-dissolved ovalbumin adsorption by using QCM. ΔF2; adsorbed amount of ovalbumin only, Δ(F1 − F2)/2; viscous load of glycerol, ΔFs; adsorbed amount of ovalbumin + viscous load of glycerol.
Mentions: The piezoelectric quartz crystal microbalance (QCM) is ahighly sensitive mass-measuring device on a surface, and has been used as abiosensor in aqueous solutions with its properties of nonlabel and real-timemonitoring. Applications of QCM instruments cover wide range of research fieldssuch as protein-protein interaction, protein-DNA interaction,material-biomolecule interaction, and material evaluations. In some cases (e.g.,protein samples dissolved in highly viscous solution such as glycerol), viscousloading significantly affected signal of mass load of protein adsorption on aQCM. Recently, we succeeded to develop the separate monitoring system of massload and viscous load by using frequency F2 measurement of QCM. In this study,we demonstrated some applications by using this system, such as viscoussolution-dissolved protein adsorption, ethanol-dissolved molecule adsorption,cation-dependent DNA conformation change, and temperature-dependentconformation change of polymer materials. We could analyze these reactions thatwere complicated by using conventional QCM system; these results can expand thepossibility of QCM instruments as biosensors (see Figure 7).

View Article: PubMed Central - PubMed

Affiliation: P S Analytical, Arthur House, Crayfields Industrial Estate, Main Road, Orpington, Kent BR5 3Hp, UK.

No MeSH data available.