Mentions: A Gilson parallel analytical chiral systemoptimizes chiral separations in a fraction of the time compared to conventionalchiral screening. The system screens chiral compounds on four separate chiralcolumns simultaneously, increasing throughput by 300%. Four analytical separations with chiral detectionin 15 minutes versus more than 60 minutes for a chiral analysis in series.The liquid handler automatically dilutes each sample to a specified concentration.Various solvents can be accessed for the dilution through a syringeless solventdelivery system, and solubilized via an orbital shaker. The liquid handlersimultaneously introduces the sample to four parallel chiral columns. Eachseparation is monitored by UV/Vis and chiral detection. The system is capableof gradient and isocratic mobile phase and mobile phase solvent selection. Onlyone chiral detector is required for the system, drastically reducing capitalinvestment. The system also monitors flow rate changes for each column throughpressure sensors. The systems' flow rate and sample are split in four ways by amanifold prior to the columns. After the sample has been chromatographed, theflow rate is combined into a single stream by a second manifold prior to thechiral detector, allowing for determination of optical rotation of theseparated peaks. Automatic overlays of the four UV traces relative to theoptical detector predict the best conditions for the separation based on typeof chiral column performance, mobile phase composition, and optical rotationwithin a single chromatographic run. Both gradient and isocratic profiles wereextremely reproducible. The system was designed to maintain the entireness ofthe separation through the parallel columns and into the chiral detector, whichis accomplished by minimizing tubing lengths and IDs to be equal throughout theentire system. The results from the analytical chiral scout chromatographicanalysis can then be implemented in preparative purification (see Figure 5).
Affiliation: P S Analytical, Arthur House, Crayfields Industrial Estate, Main Road, Orpington, Kent BR5 3Hp, UK.
No MeSH data available.