Mentions: We have recently implemented surface-induceddissociation (SID) within a Q-TOF mass spectrometer for the study of proteinassemblies. The sudden activation provided by SID results in a more symmetricproduct ion distribution and fragments other than monomer and (n-1)-mer,indicating that SID may yield additional information about the organization ofsubunits within a complex. Results are presented for SID of bacteriophage Croproteins, bovine insulin oligomers, and heat shock proteins from severalorganisms. These complexes demonstrate how the surface-induced dissociation ofprotein assemblies is affected by their molecular weight, subunit number,interfacial contact area, and subunit conformation (see Figure 4).
Affiliation: P S Analytical, Arthur House, Crayfields Industrial Estate, Main Road, Orpington, Kent BR5 3Hp, UK.
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