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The transcriptome of retinal Müller glial cells.

Roesch K, Jadhav AP, Trimarchi JM, Stadler MB, Roska B, Sun BB, Cepko CL - J. Comp. Neurol. (2008)

Bottom Line: Genes expressed exclusively by Müller glia were identified as novel markers.In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated.The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

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Related in: MedlinePlus

Cluster analysis of retinal single cells. A hierarchical cluster analysis was run on the Affymetrix signals from 19 developing amacrine (ACs) and ganglion cells (RGCs), 2 immature rod photoreceptors (PRs) from postnatal day 0 (P0), 2 mature rod photoreceptors, and 5 mature Müller glial cells (MGs). Selected portions of clusters are shown as a heatmap. Columns represent the samples, and rows represent the transcripts (with abbreviated name and Affymetrix ID). A: Part of the cluster around the gene Aqp4, revealing genes enriched in Müller glia. B: A cluster of photoreceptor specific genes.
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fig03: Cluster analysis of retinal single cells. A hierarchical cluster analysis was run on the Affymetrix signals from 19 developing amacrine (ACs) and ganglion cells (RGCs), 2 immature rod photoreceptors (PRs) from postnatal day 0 (P0), 2 mature rod photoreceptors, and 5 mature Müller glial cells (MGs). Selected portions of clusters are shown as a heatmap. Columns represent the samples, and rows represent the transcripts (with abbreviated name and Affymetrix ID). A: Part of the cluster around the gene Aqp4, revealing genes enriched in Müller glia. B: A cluster of photoreceptor specific genes.

Mentions: A representative part of the resulting expression level matrix is shown in Supplemental Figure S1 and a portion of that same cluster around Aqp4 is shown enlarged in Figure 3A. Interestingly, tight clustering of genes in a given cell type can be observed, with Figure 3A showing a portion of a Müller glial cluster, and Figure 3B showing a rod photoreceptor cluster. All Müller glial cells shared a similar gene expression profile over a wide range of genes (Supplemental Fig. S1). Other cell types showed expression values that were considerably distinct from those of the Müller glia. The data in Figure 3A of genes enriched in Müller glia suggest a Müller glia core transcriptome, which likely comprises genes encoding some of the key functions of Müller glia. One of the most prominent examples is the inwardly rectifying potassium channel (Kcnj10). Müller glia have been previously shown to play an active role in regulating retinal functions by allowing transport of small molecules through specific ion channels. Other potassium channels expressed in Müller glial cells include Kir4.1 and Kir2.1 (Newman and Reichenbach, 1996; Bringmann et al., 2000). Genes included in this cluster are also ApoE and Abca8a, which have been identified to be among the most highly expressed genes in the previous analysis (Fig. 2). Genes not previously identified in Müller glia, such as two members of the lysosomal cystein protease family, cathepsin O (Ctso) and cathepsin H (Ctsh), and rhophilin, which is involved in actin cytoskeleton reorganization, have been found to be present in this cluster as well.


The transcriptome of retinal Müller glial cells.

Roesch K, Jadhav AP, Trimarchi JM, Stadler MB, Roska B, Sun BB, Cepko CL - J. Comp. Neurol. (2008)

Cluster analysis of retinal single cells. A hierarchical cluster analysis was run on the Affymetrix signals from 19 developing amacrine (ACs) and ganglion cells (RGCs), 2 immature rod photoreceptors (PRs) from postnatal day 0 (P0), 2 mature rod photoreceptors, and 5 mature Müller glial cells (MGs). Selected portions of clusters are shown as a heatmap. Columns represent the samples, and rows represent the transcripts (with abbreviated name and Affymetrix ID). A: Part of the cluster around the gene Aqp4, revealing genes enriched in Müller glia. B: A cluster of photoreceptor specific genes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2665263&req=5

fig03: Cluster analysis of retinal single cells. A hierarchical cluster analysis was run on the Affymetrix signals from 19 developing amacrine (ACs) and ganglion cells (RGCs), 2 immature rod photoreceptors (PRs) from postnatal day 0 (P0), 2 mature rod photoreceptors, and 5 mature Müller glial cells (MGs). Selected portions of clusters are shown as a heatmap. Columns represent the samples, and rows represent the transcripts (with abbreviated name and Affymetrix ID). A: Part of the cluster around the gene Aqp4, revealing genes enriched in Müller glia. B: A cluster of photoreceptor specific genes.
Mentions: A representative part of the resulting expression level matrix is shown in Supplemental Figure S1 and a portion of that same cluster around Aqp4 is shown enlarged in Figure 3A. Interestingly, tight clustering of genes in a given cell type can be observed, with Figure 3A showing a portion of a Müller glial cluster, and Figure 3B showing a rod photoreceptor cluster. All Müller glial cells shared a similar gene expression profile over a wide range of genes (Supplemental Fig. S1). Other cell types showed expression values that were considerably distinct from those of the Müller glia. The data in Figure 3A of genes enriched in Müller glia suggest a Müller glia core transcriptome, which likely comprises genes encoding some of the key functions of Müller glia. One of the most prominent examples is the inwardly rectifying potassium channel (Kcnj10). Müller glia have been previously shown to play an active role in regulating retinal functions by allowing transport of small molecules through specific ion channels. Other potassium channels expressed in Müller glial cells include Kir4.1 and Kir2.1 (Newman and Reichenbach, 1996; Bringmann et al., 2000). Genes included in this cluster are also ApoE and Abca8a, which have been identified to be among the most highly expressed genes in the previous analysis (Fig. 2). Genes not previously identified in Müller glia, such as two members of the lysosomal cystein protease family, cathepsin O (Ctso) and cathepsin H (Ctsh), and rhophilin, which is involved in actin cytoskeleton reorganization, have been found to be present in this cluster as well.

Bottom Line: Genes expressed exclusively by Müller glia were identified as novel markers.In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated.The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Show MeSH
Related in: MedlinePlus