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Corneal allograft endothelial cell replacement represents a reparative response to transplant injury.

Gong N, Pleyer U, Ritter T, Knop E, Chen X - Mol. Vis. (2009)

Bottom Line: The corneal transplantation model in an major histocompatibility complex (MHC) class I/II disparate Dark Agouti (DA)-Lewis combination was used.The rejection kinetics was observed in 16 cases in which the corneal opacity grade was recorded after grafting and after the onset of rejection.Moreover, although the whole endothelium would be fully recovered after rejection, the ratio of regenerative EC density reached only half of normal levels as long as six months after the transplant.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Purpose: To elucidate the injury of corneal allograft endothelial cells (ECs) upon rejection and the subsequent replacement process of the cells.

Methods: The corneal transplantation model in an major histocompatibility complex (MHC) class I/II disparate Dark Agouti (DA)-Lewis combination was used. The rejection kinetics was observed in 16 cases in which the corneal opacity grade was recorded after grafting and after the onset of rejection. Four normal corneas and four allografts were subjected to EC staining to investigate the EC integrity in cases of rejection. Furthermore, a series of rejected allografts were examined and the EC integrity compared at one week, three weeks, three months, and six months after the onset of rejection.

Results: All corneal allografts were rejected, resulting in EC integrity loss. However, the allografts recovered transparency around 18 days after the onset of rejection with repaired endothelium by regenerative ECs. Moreover, although the whole endothelium would be fully recovered after rejection, the ratio of regenerative EC density reached only half of normal levels as long as six months after the transplant.

Conclusions: Corneal allograft EC replacement represents a reparative response to transplant-related injury.

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Related in: MedlinePlus

The effect of rejection upon corneal allografts. A: The rejection kinetics of corneal transplantation in the DA-Lewis combination is shown. Sixteen Lewis rats served as recipients of DA allografts. The opacity grade 3 or 4 is defined as rejection onset. The rejection happened in all allografts from day 11 to day 14 with an MST of 13.1±0.3 days (n=16). Meanwhile, no rejection was observed in any of the isografts (n=6). B: The EC staining in a normal DA cornea is displayed. The cells were distributed regularly, and their nuclei exhibited similar shape (magnification 400X). C: The EC staining in rejected corneal allograft is shown. All ECs were lost except the remaining apoptotic cell (400X).
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f1: The effect of rejection upon corneal allografts. A: The rejection kinetics of corneal transplantation in the DA-Lewis combination is shown. Sixteen Lewis rats served as recipients of DA allografts. The opacity grade 3 or 4 is defined as rejection onset. The rejection happened in all allografts from day 11 to day 14 with an MST of 13.1±0.3 days (n=16). Meanwhile, no rejection was observed in any of the isografts (n=6). B: The EC staining in a normal DA cornea is displayed. The cells were distributed regularly, and their nuclei exhibited similar shape (magnification 400X). C: The EC staining in rejected corneal allograft is shown. All ECs were lost except the remaining apoptotic cell (400X).

Mentions: To investigate the rejection kinetics of the MHC class I/II disparate DA-Lewis corneal transplant, 16 Lewis rats were employed as the recipients of DA corneal grafts. No treatment was given to either the donors or the recipients. During the first week after transplantation, the grafts had slight stroma edema but then recovered clarity within one week. Afterward, their opacity grade increased gradually to 3 or 4, which was defined as rejection onset. The rejection kinetics of these allografts is shown in Figure 1A. The rejection happened in all allografts from day 11 to day 14 (MST 13.1±0.3 days, n=16). In the isograft control group, except when the slight edema happened due to surgical trauma, all transplants retained clarity in the observation of four weeks, showing that the opacity grade increase was attributable to the immunological response.


Corneal allograft endothelial cell replacement represents a reparative response to transplant injury.

Gong N, Pleyer U, Ritter T, Knop E, Chen X - Mol. Vis. (2009)

The effect of rejection upon corneal allografts. A: The rejection kinetics of corneal transplantation in the DA-Lewis combination is shown. Sixteen Lewis rats served as recipients of DA allografts. The opacity grade 3 or 4 is defined as rejection onset. The rejection happened in all allografts from day 11 to day 14 with an MST of 13.1±0.3 days (n=16). Meanwhile, no rejection was observed in any of the isografts (n=6). B: The EC staining in a normal DA cornea is displayed. The cells were distributed regularly, and their nuclei exhibited similar shape (magnification 400X). C: The EC staining in rejected corneal allograft is shown. All ECs were lost except the remaining apoptotic cell (400X).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2664843&req=5

f1: The effect of rejection upon corneal allografts. A: The rejection kinetics of corneal transplantation in the DA-Lewis combination is shown. Sixteen Lewis rats served as recipients of DA allografts. The opacity grade 3 or 4 is defined as rejection onset. The rejection happened in all allografts from day 11 to day 14 with an MST of 13.1±0.3 days (n=16). Meanwhile, no rejection was observed in any of the isografts (n=6). B: The EC staining in a normal DA cornea is displayed. The cells were distributed regularly, and their nuclei exhibited similar shape (magnification 400X). C: The EC staining in rejected corneal allograft is shown. All ECs were lost except the remaining apoptotic cell (400X).
Mentions: To investigate the rejection kinetics of the MHC class I/II disparate DA-Lewis corneal transplant, 16 Lewis rats were employed as the recipients of DA corneal grafts. No treatment was given to either the donors or the recipients. During the first week after transplantation, the grafts had slight stroma edema but then recovered clarity within one week. Afterward, their opacity grade increased gradually to 3 or 4, which was defined as rejection onset. The rejection kinetics of these allografts is shown in Figure 1A. The rejection happened in all allografts from day 11 to day 14 (MST 13.1±0.3 days, n=16). In the isograft control group, except when the slight edema happened due to surgical trauma, all transplants retained clarity in the observation of four weeks, showing that the opacity grade increase was attributable to the immunological response.

Bottom Line: The corneal transplantation model in an major histocompatibility complex (MHC) class I/II disparate Dark Agouti (DA)-Lewis combination was used.The rejection kinetics was observed in 16 cases in which the corneal opacity grade was recorded after grafting and after the onset of rejection.Moreover, although the whole endothelium would be fully recovered after rejection, the ratio of regenerative EC density reached only half of normal levels as long as six months after the transplant.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Purpose: To elucidate the injury of corneal allograft endothelial cells (ECs) upon rejection and the subsequent replacement process of the cells.

Methods: The corneal transplantation model in an major histocompatibility complex (MHC) class I/II disparate Dark Agouti (DA)-Lewis combination was used. The rejection kinetics was observed in 16 cases in which the corneal opacity grade was recorded after grafting and after the onset of rejection. Four normal corneas and four allografts were subjected to EC staining to investigate the EC integrity in cases of rejection. Furthermore, a series of rejected allografts were examined and the EC integrity compared at one week, three weeks, three months, and six months after the onset of rejection.

Results: All corneal allografts were rejected, resulting in EC integrity loss. However, the allografts recovered transparency around 18 days after the onset of rejection with repaired endothelium by regenerative ECs. Moreover, although the whole endothelium would be fully recovered after rejection, the ratio of regenerative EC density reached only half of normal levels as long as six months after the transplant.

Conclusions: Corneal allograft EC replacement represents a reparative response to transplant-related injury.

Show MeSH
Related in: MedlinePlus