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Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity.

Frank MB, Yang Q, Osban J, Azzarello JT, Saban MR, Saban R, Ashley RA, Welter JC, Fung KM, Lin HK - BMC Complement Altern Med (2009)

Bottom Line: Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees.Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells.However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. frank@omrf.org

ABSTRACT

Background: Originating from Africa, India, and the Middle East, frankincense oil has been important both socially and economically as an ingredient in incense and perfumes for thousands of years. Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees. One of the main components of frankincense oil is boswellic acid, a component known to have anti-neoplastic properties. The goal of this study was to evaluate frankincense oil for its anti-tumor activity and signaling pathways in bladder cancer cells.

Methods: Frankincense oil-induced cell viability was investigated in human bladder cancer J82 cells and immortalized normal bladder urothelial UROtsa cells. Temporal regulation of frankincense oil-activated gene expression in bladder cancer cells was identified by microarray and bioinformatics analysis.

Results: Within a range of concentration, frankincense oil suppressed cell viability in bladder transitional carcinoma J82 cells but not in UROtsa cells. Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells. However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

Conclusion: Frankincense oil appears to distinguish cancerous from normal bladder cells and suppress cancer cell viability. Microarray and bioinformatics analysis proposed multiple pathways that can be activated by frankincense oil to induce bladder cancer cell death. Frankincense oil might represent an alternative intravesical agent for bladder cancer treatment.

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Related in: MedlinePlus

Frankincense oil-induced J82 cell death. To determine whether frankincense oil-induced apoptosis in bladder cancer cells, J82 cells were seeded in 60 mm tissue culture plates at the concentration of 2 × 105 cells per plate, cultured overnight for adherence, and either left untreated or treated with 1:1,000 dilution of frankincense oil. (A) TUNEL analysis was performed at 3 hours following treatment. Apoptotic cells with damaged DNA were stained positive with a bright red color (inserts). (B) DNA fragmentation was determined by separating genomic DNA on a 2% agaorse gel; and the gel image was captured using Gel Doc 100 system (Bio-Rad, Hercules, CA).
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Figure 4: Frankincense oil-induced J82 cell death. To determine whether frankincense oil-induced apoptosis in bladder cancer cells, J82 cells were seeded in 60 mm tissue culture plates at the concentration of 2 × 105 cells per plate, cultured overnight for adherence, and either left untreated or treated with 1:1,000 dilution of frankincense oil. (A) TUNEL analysis was performed at 3 hours following treatment. Apoptotic cells with damaged DNA were stained positive with a bright red color (inserts). (B) DNA fragmentation was determined by separating genomic DNA on a 2% agaorse gel; and the gel image was captured using Gel Doc 100 system (Bio-Rad, Hercules, CA).

Mentions: TUNEL analysis was performed to determine whether frankincense oil treated J82 cells undergo apoptosis. Frankincense oil treatment resulted in an increased number of bright red colored TUNEL positive cells as compared to untreated cells (Figure 4A). Genomic DNA fragmentation was determined between hours 1 and 6 in J82 cells following frankincense oil treatment. Agarose gel electrophoresis results showed that all genomic DNA remained as large pieces of DNA without forming a small DNA ladder (Figure 4B). There was no detectable genomic DNA for J82 cells harvested at 12 hours following frankincense oil treatment (data not shown).


Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity.

Frank MB, Yang Q, Osban J, Azzarello JT, Saban MR, Saban R, Ashley RA, Welter JC, Fung KM, Lin HK - BMC Complement Altern Med (2009)

Frankincense oil-induced J82 cell death. To determine whether frankincense oil-induced apoptosis in bladder cancer cells, J82 cells were seeded in 60 mm tissue culture plates at the concentration of 2 × 105 cells per plate, cultured overnight for adherence, and either left untreated or treated with 1:1,000 dilution of frankincense oil. (A) TUNEL analysis was performed at 3 hours following treatment. Apoptotic cells with damaged DNA were stained positive with a bright red color (inserts). (B) DNA fragmentation was determined by separating genomic DNA on a 2% agaorse gel; and the gel image was captured using Gel Doc 100 system (Bio-Rad, Hercules, CA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2664784&req=5

Figure 4: Frankincense oil-induced J82 cell death. To determine whether frankincense oil-induced apoptosis in bladder cancer cells, J82 cells were seeded in 60 mm tissue culture plates at the concentration of 2 × 105 cells per plate, cultured overnight for adherence, and either left untreated or treated with 1:1,000 dilution of frankincense oil. (A) TUNEL analysis was performed at 3 hours following treatment. Apoptotic cells with damaged DNA were stained positive with a bright red color (inserts). (B) DNA fragmentation was determined by separating genomic DNA on a 2% agaorse gel; and the gel image was captured using Gel Doc 100 system (Bio-Rad, Hercules, CA).
Mentions: TUNEL analysis was performed to determine whether frankincense oil treated J82 cells undergo apoptosis. Frankincense oil treatment resulted in an increased number of bright red colored TUNEL positive cells as compared to untreated cells (Figure 4A). Genomic DNA fragmentation was determined between hours 1 and 6 in J82 cells following frankincense oil treatment. Agarose gel electrophoresis results showed that all genomic DNA remained as large pieces of DNA without forming a small DNA ladder (Figure 4B). There was no detectable genomic DNA for J82 cells harvested at 12 hours following frankincense oil treatment (data not shown).

Bottom Line: Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees.Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells.However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. frank@omrf.org

ABSTRACT

Background: Originating from Africa, India, and the Middle East, frankincense oil has been important both socially and economically as an ingredient in incense and perfumes for thousands of years. Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees. One of the main components of frankincense oil is boswellic acid, a component known to have anti-neoplastic properties. The goal of this study was to evaluate frankincense oil for its anti-tumor activity and signaling pathways in bladder cancer cells.

Methods: Frankincense oil-induced cell viability was investigated in human bladder cancer J82 cells and immortalized normal bladder urothelial UROtsa cells. Temporal regulation of frankincense oil-activated gene expression in bladder cancer cells was identified by microarray and bioinformatics analysis.

Results: Within a range of concentration, frankincense oil suppressed cell viability in bladder transitional carcinoma J82 cells but not in UROtsa cells. Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells. However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

Conclusion: Frankincense oil appears to distinguish cancerous from normal bladder cells and suppress cancer cell viability. Microarray and bioinformatics analysis proposed multiple pathways that can be activated by frankincense oil to induce bladder cancer cell death. Frankincense oil might represent an alternative intravesical agent for bladder cancer treatment.

Show MeSH
Related in: MedlinePlus