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Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity.

Frank MB, Yang Q, Osban J, Azzarello JT, Saban MR, Saban R, Ashley RA, Welter JC, Fung KM, Lin HK - BMC Complement Altern Med (2009)

Bottom Line: Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees.Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells.However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. frank@omrf.org

ABSTRACT

Background: Originating from Africa, India, and the Middle East, frankincense oil has been important both socially and economically as an ingredient in incense and perfumes for thousands of years. Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees. One of the main components of frankincense oil is boswellic acid, a component known to have anti-neoplastic properties. The goal of this study was to evaluate frankincense oil for its anti-tumor activity and signaling pathways in bladder cancer cells.

Methods: Frankincense oil-induced cell viability was investigated in human bladder cancer J82 cells and immortalized normal bladder urothelial UROtsa cells. Temporal regulation of frankincense oil-activated gene expression in bladder cancer cells was identified by microarray and bioinformatics analysis.

Results: Within a range of concentration, frankincense oil suppressed cell viability in bladder transitional carcinoma J82 cells but not in UROtsa cells. Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells. However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

Conclusion: Frankincense oil appears to distinguish cancerous from normal bladder cells and suppress cancer cell viability. Microarray and bioinformatics analysis proposed multiple pathways that can be activated by frankincense oil to induce bladder cancer cell death. Frankincense oil might represent an alternative intravesical agent for bladder cancer treatment.

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Hierarchical clustering of frankincense oil-regulated apoptosis-related genes in J82 cells. The map was obtained using Biometric Research Branch (BRB) ArrayTools version 3.4.0 – Beta_2 software  after log2 transformation of fluorescence intensities. Each column represents time intervals following frankincense oil exposure, and each row represents a gene probe set. The expression levels for individual genes are indicated by green/red color indicating an elevated/suppressed level of expression, respectively.
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Figure 3: Hierarchical clustering of frankincense oil-regulated apoptosis-related genes in J82 cells. The map was obtained using Biometric Research Branch (BRB) ArrayTools version 3.4.0 – Beta_2 software after log2 transformation of fluorescence intensities. Each column represents time intervals following frankincense oil exposure, and each row represents a gene probe set. The expression levels for individual genes are indicated by green/red color indicating an elevated/suppressed level of expression, respectively.

Mentions: Levels of a large number of genes that are responsible for apoptosis were found to be modulated by frankincense oil (Figure 3). These genes included CDKN1A, DEDD2, IER3, IL6, SGK, and TNFAIP3 (up-regulated between 1 and 2 hours and remained up-regulated) as well as GAD45B, NUDT2, and others (up-regulated between 2 and 3 hours). In addition, the cell survival gene, AXL, was down-regulated by frankincense oil. However, two anti-apoptotic genes, GSTP1 and IL1A, were up-regulated. A similar contradiction was seen with a pro-apoptotic gene, ING4, being down-regulated.


Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity.

Frank MB, Yang Q, Osban J, Azzarello JT, Saban MR, Saban R, Ashley RA, Welter JC, Fung KM, Lin HK - BMC Complement Altern Med (2009)

Hierarchical clustering of frankincense oil-regulated apoptosis-related genes in J82 cells. The map was obtained using Biometric Research Branch (BRB) ArrayTools version 3.4.0 – Beta_2 software  after log2 transformation of fluorescence intensities. Each column represents time intervals following frankincense oil exposure, and each row represents a gene probe set. The expression levels for individual genes are indicated by green/red color indicating an elevated/suppressed level of expression, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2664784&req=5

Figure 3: Hierarchical clustering of frankincense oil-regulated apoptosis-related genes in J82 cells. The map was obtained using Biometric Research Branch (BRB) ArrayTools version 3.4.0 – Beta_2 software after log2 transformation of fluorescence intensities. Each column represents time intervals following frankincense oil exposure, and each row represents a gene probe set. The expression levels for individual genes are indicated by green/red color indicating an elevated/suppressed level of expression, respectively.
Mentions: Levels of a large number of genes that are responsible for apoptosis were found to be modulated by frankincense oil (Figure 3). These genes included CDKN1A, DEDD2, IER3, IL6, SGK, and TNFAIP3 (up-regulated between 1 and 2 hours and remained up-regulated) as well as GAD45B, NUDT2, and others (up-regulated between 2 and 3 hours). In addition, the cell survival gene, AXL, was down-regulated by frankincense oil. However, two anti-apoptotic genes, GSTP1 and IL1A, were up-regulated. A similar contradiction was seen with a pro-apoptotic gene, ING4, being down-regulated.

Bottom Line: Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees.Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells.However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. frank@omrf.org

ABSTRACT

Background: Originating from Africa, India, and the Middle East, frankincense oil has been important both socially and economically as an ingredient in incense and perfumes for thousands of years. Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees. One of the main components of frankincense oil is boswellic acid, a component known to have anti-neoplastic properties. The goal of this study was to evaluate frankincense oil for its anti-tumor activity and signaling pathways in bladder cancer cells.

Methods: Frankincense oil-induced cell viability was investigated in human bladder cancer J82 cells and immortalized normal bladder urothelial UROtsa cells. Temporal regulation of frankincense oil-activated gene expression in bladder cancer cells was identified by microarray and bioinformatics analysis.

Results: Within a range of concentration, frankincense oil suppressed cell viability in bladder transitional carcinoma J82 cells but not in UROtsa cells. Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells. However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.

Conclusion: Frankincense oil appears to distinguish cancerous from normal bladder cells and suppress cancer cell viability. Microarray and bioinformatics analysis proposed multiple pathways that can be activated by frankincense oil to induce bladder cancer cell death. Frankincense oil might represent an alternative intravesical agent for bladder cancer treatment.

Show MeSH
Related in: MedlinePlus