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Impact of cytomegalovirus and grafts versus host disease on the dynamics of CD57+CD28-CD8+ T cells after bone marrow transplant.

Mendes AV, Kallas EG, Benard G, Pannuti CS, Menezes R, Dulley FL, Evans TG, Salomão R, Machado CM - Clinics (Sao Paulo) (2008)

Bottom Line: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients.Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05).This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. avamendes@gmail.com

ABSTRACT

Objectives: The present study aimed to evaluate the dynamics of CD28 and CD57 expression in CD8+ T lymphocytes during cytomegalovirus viremia in bone marrow transplant recipients.

Methods: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients. To evaluate the expression of CD57 and CD28 on CD8+ T lymphocytes, flow cytometry analysis was performed on blood samples for four months after bone marrow transplant, together with cytomegalovirus antigenemia assays.

Results: Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05). A linear regression model demonstrated a continuous decrease in CD28+ expression and a continuous increase in CD57+ expression after bone marrow transplant. The occurrence of cytomegalovirus antigenemia was associated with a steep drop in the percentage of CD28+ cells (5.94%, p<0.01) and an increase in CD57+ lymphocytes (5.60%, p<0.01). This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients. The development of acute graft versus host disease, which occurred at an earlier time than antigenemia (day 26 vs. day 56 post- bone marrow transplant), also had an impact on the CD57+ subset, triggering an increase of 4.9% in CD57+ lymphocytes (p<0.05).

Conclusion: We found continuous relative changes in the CD28+ and CD57+ subsets during the first 120 days post- bone marrow transplant, as part of immune system reconstitution and maturation. A clear correlation was observed between the expansion of the CD57+CD28-CD8+ T lymphocyte subpopulation and the occurrence of graft versus host disease and cytomegalovirus viremia.

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Relative decrease of CD57−CD28+ subset (A) and a relative increase of CD57+CD28− subset (B) in the CD8+ T lymphocyte population occur continuously after BMT, as assessed by Spearman’s correlation test
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f2-16-0109: Relative decrease of CD57−CD28+ subset (A) and a relative increase of CD57+CD28− subset (B) in the CD8+ T lymphocyte population occur continuously after BMT, as assessed by Spearman’s correlation test

Mentions: Using a linear regression model, the dynamics of the CD28+ and CD57+ subpopulations within CD8+ T lymphocytes were analyzed. In general, after BMT, the percentage of CD28+ cells decreased, while that of CD57+ cells increased. These changes occurred at a relatively constant rate, independent of transplant type or the occurrence of CMV viremia or GVHD (Figure 2). On average, the baseline level of CD28+ cells (measured early during engraftment) was higher in the allogeneic BMT patients (72.11% at baseline, with −0.38%/day thereafter) than in the autologous BMT patients (46.23% at baseline, with −0.17%/day thereafter). Consistent with their mutually exclusive expression, the baseline level of CD57+ was lower in the allogeneic BMT group (14.40% at baseline, with +0.27%/day thereafter) than in the autologous BMT group (18.42% at baseline, with +0.12%/day thereafter).


Impact of cytomegalovirus and grafts versus host disease on the dynamics of CD57+CD28-CD8+ T cells after bone marrow transplant.

Mendes AV, Kallas EG, Benard G, Pannuti CS, Menezes R, Dulley FL, Evans TG, Salomão R, Machado CM - Clinics (Sao Paulo) (2008)

Relative decrease of CD57−CD28+ subset (A) and a relative increase of CD57+CD28− subset (B) in the CD8+ T lymphocyte population occur continuously after BMT, as assessed by Spearman’s correlation test
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664726&req=5

f2-16-0109: Relative decrease of CD57−CD28+ subset (A) and a relative increase of CD57+CD28− subset (B) in the CD8+ T lymphocyte population occur continuously after BMT, as assessed by Spearman’s correlation test
Mentions: Using a linear regression model, the dynamics of the CD28+ and CD57+ subpopulations within CD8+ T lymphocytes were analyzed. In general, after BMT, the percentage of CD28+ cells decreased, while that of CD57+ cells increased. These changes occurred at a relatively constant rate, independent of transplant type or the occurrence of CMV viremia or GVHD (Figure 2). On average, the baseline level of CD28+ cells (measured early during engraftment) was higher in the allogeneic BMT patients (72.11% at baseline, with −0.38%/day thereafter) than in the autologous BMT patients (46.23% at baseline, with −0.17%/day thereafter). Consistent with their mutually exclusive expression, the baseline level of CD57+ was lower in the allogeneic BMT group (14.40% at baseline, with +0.27%/day thereafter) than in the autologous BMT group (18.42% at baseline, with +0.12%/day thereafter).

Bottom Line: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients.Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05).This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Virologia, Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. avamendes@gmail.com

ABSTRACT

Objectives: The present study aimed to evaluate the dynamics of CD28 and CD57 expression in CD8+ T lymphocytes during cytomegalovirus viremia in bone marrow transplant recipients.

Methods: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients. To evaluate the expression of CD57 and CD28 on CD8+ T lymphocytes, flow cytometry analysis was performed on blood samples for four months after bone marrow transplant, together with cytomegalovirus antigenemia assays.

Results: Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05). A linear regression model demonstrated a continuous decrease in CD28+ expression and a continuous increase in CD57+ expression after bone marrow transplant. The occurrence of cytomegalovirus antigenemia was associated with a steep drop in the percentage of CD28+ cells (5.94%, p<0.01) and an increase in CD57+ lymphocytes (5.60%, p<0.01). This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients. The development of acute graft versus host disease, which occurred at an earlier time than antigenemia (day 26 vs. day 56 post- bone marrow transplant), also had an impact on the CD57+ subset, triggering an increase of 4.9% in CD57+ lymphocytes (p<0.05).

Conclusion: We found continuous relative changes in the CD28+ and CD57+ subsets during the first 120 days post- bone marrow transplant, as part of immune system reconstitution and maturation. A clear correlation was observed between the expansion of the CD57+CD28-CD8+ T lymphocyte subpopulation and the occurrence of graft versus host disease and cytomegalovirus viremia.

Show MeSH
Related in: MedlinePlus