Limits...
Viral vector induction of CREB expression in the periaqueductal gray induces a predator stress-like pattern of changes in pCREB expression, neuroplasticity, and anxiety in rodents.

Adamec R, Berton O, Abdul Razek W - Neural Plast. (2009)

Bottom Line: Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray).Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation.Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.

ABSTRACT
Predator stress is lastingly anxiogenic. Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray). Predator stress also potentiates right but not left CeA-PAG (central amygdala-PAG) transmission up to 12 days after stress. The present study explored the functional significance of pCREB changes by increasing CREB expression in non-predator stressed rats through viral vectoring, and assessing the behavioral, electrophysiological and pCREB expression changes in comparison with handled and predator stressed controls. Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation. Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected. The findings of this study suggest that by increasing CREB and pCREB in the right lateral PAG, it is possible to produce rats that exhibit behavioral, brain, and molecular changes that closely resemble those seen in predator stressed rats.

Show MeSH

Related in: MedlinePlus

(a) shows example fits (solid line) to an FFT smoothed (20%) function (dashedline) of the means of peak startle amplitude (dotted line) over 20 trials forHandled GFP control (left) and predator stressed (right) rats. Plotted in (b) are median peak startle amplitudes (left) and 									τ ± SE									 (right),estimated from declining exponential functions, for rats in each experimentalgroup. Medians and Tau values marked with a different letter differ from eachother (P < .05).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2664642&req=5

fig3: (a) shows example fits (solid line) to an FFT smoothed (20%) function (dashedline) of the means of peak startle amplitude (dotted line) over 20 trials forHandled GFP control (left) and predator stressed (right) rats. Plotted in (b) are median peak startle amplitudes (left) and τ ± SE (right),estimated from declining exponential functions, for rats in each experimentalgroup. Medians and Tau values marked with a different letter differ from eachother (P < .05).

Mentions: Between groups startle data were notnormally distributed (Omnibus Normality Test = 148.07, P < .0001). Therefore, Kruskal-Wallis one way non-parametricANOVA on medians of peak startle amplitude over trials was used. Groupsdiffered (χ2 (2) = 119.90, P < .001). Planned comparisons(Kruskal-Wallis multiple comparison z-test z > 3.98, P < .01)revealed that predator stress increased startle over both handled groups(Figure 3, bottom left panel). Nevertheless startle amplitude of Handled CREB rats was alsohigher than Handled GFP, but lower than predator stressed animals (Figure 3,(b) left panel).


Viral vector induction of CREB expression in the periaqueductal gray induces a predator stress-like pattern of changes in pCREB expression, neuroplasticity, and anxiety in rodents.

Adamec R, Berton O, Abdul Razek W - Neural Plast. (2009)

(a) shows example fits (solid line) to an FFT smoothed (20%) function (dashedline) of the means of peak startle amplitude (dotted line) over 20 trials forHandled GFP control (left) and predator stressed (right) rats. Plotted in (b) are median peak startle amplitudes (left) and 									τ ± SE									 (right),estimated from declining exponential functions, for rats in each experimentalgroup. Medians and Tau values marked with a different letter differ from eachother (P < .05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664642&req=5

fig3: (a) shows example fits (solid line) to an FFT smoothed (20%) function (dashedline) of the means of peak startle amplitude (dotted line) over 20 trials forHandled GFP control (left) and predator stressed (right) rats. Plotted in (b) are median peak startle amplitudes (left) and τ ± SE (right),estimated from declining exponential functions, for rats in each experimentalgroup. Medians and Tau values marked with a different letter differ from eachother (P < .05).
Mentions: Between groups startle data were notnormally distributed (Omnibus Normality Test = 148.07, P < .0001). Therefore, Kruskal-Wallis one way non-parametricANOVA on medians of peak startle amplitude over trials was used. Groupsdiffered (χ2 (2) = 119.90, P < .001). Planned comparisons(Kruskal-Wallis multiple comparison z-test z > 3.98, P < .01)revealed that predator stress increased startle over both handled groups(Figure 3, bottom left panel). Nevertheless startle amplitude of Handled CREB rats was alsohigher than Handled GFP, but lower than predator stressed animals (Figure 3,(b) left panel).

Bottom Line: Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray).Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation.Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.

ABSTRACT
Predator stress is lastingly anxiogenic. Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray). Predator stress also potentiates right but not left CeA-PAG (central amygdala-PAG) transmission up to 12 days after stress. The present study explored the functional significance of pCREB changes by increasing CREB expression in non-predator stressed rats through viral vectoring, and assessing the behavioral, electrophysiological and pCREB expression changes in comparison with handled and predator stressed controls. Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation. Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected. The findings of this study suggest that by increasing CREB and pCREB in the right lateral PAG, it is possible to produce rats that exhibit behavioral, brain, and molecular changes that closely resemble those seen in predator stressed rats.

Show MeSH
Related in: MedlinePlus