Limits...
Viral vector induction of CREB expression in the periaqueductal gray induces a predator stress-like pattern of changes in pCREB expression, neuroplasticity, and anxiety in rodents.

Adamec R, Berton O, Abdul Razek W - Neural Plast. (2009)

Bottom Line: Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray).Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation.Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.

ABSTRACT
Predator stress is lastingly anxiogenic. Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray). Predator stress also potentiates right but not left CeA-PAG (central amygdala-PAG) transmission up to 12 days after stress. The present study explored the functional significance of pCREB changes by increasing CREB expression in non-predator stressed rats through viral vectoring, and assessing the behavioral, electrophysiological and pCREB expression changes in comparison with handled and predator stressed controls. Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation. Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected. The findings of this study suggest that by increasing CREB and pCREB in the right lateral PAG, it is possible to produce rats that exhibit behavioral, brain, and molecular changes that closely resemble those seen in predator stressed rats.

Show MeSH

Related in: MedlinePlus

Plotted over groups are mean ± SEM of  EPM and hole board behaviors. Means markedwith a different letter differ from each other (P < .05). (a), shows frequency of closed arm entries (left)and ratio time (right) in the EPM. (b),  shows the frequency of rears(left) and head dips (right) in the hole board test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2664642&req=5

fig1: Plotted over groups are mean ± SEM of EPM and hole board behaviors. Means markedwith a different letter differ from each other (P < .05). (a), shows frequency of closed arm entries (left)and ratio time (right) in the EPM. (b), shows the frequency of rears(left) and head dips (right) in the hole board test.

Mentions: Groupsdiffered in the measures of open arm exploration-ratio time and ratio entry(all P (2,9) ≥ 14.78, P < .002). Predator stress reducedratio time and ratio entry in the EPM, consistent with many past studies(Figure 1, upper right panel, ratio time only is shown, ratio entry findingswere very similar). Predator stress reduced open arm exploration (increasedanxiety) the most relative to controls (Handled GFP). Injection of HSV-CREB in the right PAG alone was alsoanxiogenic in the EPM, reducing ratio time and entries in Handled-CREB rat to a level between Handled GFP andPredator Stressed GFP rats (Figure 1, Tukey Kramer test, P < .05).


Viral vector induction of CREB expression in the periaqueductal gray induces a predator stress-like pattern of changes in pCREB expression, neuroplasticity, and anxiety in rodents.

Adamec R, Berton O, Abdul Razek W - Neural Plast. (2009)

Plotted over groups are mean ± SEM of  EPM and hole board behaviors. Means markedwith a different letter differ from each other (P < .05). (a), shows frequency of closed arm entries (left)and ratio time (right) in the EPM. (b),  shows the frequency of rears(left) and head dips (right) in the hole board test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664642&req=5

fig1: Plotted over groups are mean ± SEM of EPM and hole board behaviors. Means markedwith a different letter differ from each other (P < .05). (a), shows frequency of closed arm entries (left)and ratio time (right) in the EPM. (b), shows the frequency of rears(left) and head dips (right) in the hole board test.
Mentions: Groupsdiffered in the measures of open arm exploration-ratio time and ratio entry(all P (2,9) ≥ 14.78, P < .002). Predator stress reducedratio time and ratio entry in the EPM, consistent with many past studies(Figure 1, upper right panel, ratio time only is shown, ratio entry findingswere very similar). Predator stress reduced open arm exploration (increasedanxiety) the most relative to controls (Handled GFP). Injection of HSV-CREB in the right PAG alone was alsoanxiogenic in the EPM, reducing ratio time and entries in Handled-CREB rat to a level between Handled GFP andPredator Stressed GFP rats (Figure 1, Tukey Kramer test, P < .05).

Bottom Line: Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray).Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation.Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.

ABSTRACT
Predator stress is lastingly anxiogenic. Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray). Predator stress also potentiates right but not left CeA-PAG (central amygdala-PAG) transmission up to 12 days after stress. The present study explored the functional significance of pCREB changes by increasing CREB expression in non-predator stressed rats through viral vectoring, and assessing the behavioral, electrophysiological and pCREB expression changes in comparison with handled and predator stressed controls. Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation. Potentiation of the right but not left CeA-PAG pathway was also observed. pCREB expression was slightly elevated in the right lateral column of the PAG, while the dorsal and ventral columns were not affected. The findings of this study suggest that by increasing CREB and pCREB in the right lateral PAG, it is possible to produce rats that exhibit behavioral, brain, and molecular changes that closely resemble those seen in predator stressed rats.

Show MeSH
Related in: MedlinePlus