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Gold-Speckled Multimodal Nanoparticles for Noninvasive Bioimaging.

Sharma P, Brown SC, Bengtsson N, Zhang Q, Walter GA, Grobmyer SR, Santra S, Jiang H, Scott EW, Moudgil BM - Chem Mater (2008)

Bottom Line: Multimodal Gold-speckled silica nanoparticles as contrast agents for noninvasive imaging with magnetic resonance imaging and photoacoustic tomography have been prepared in a simple one-pot synthesis using nonionic microemulsions.Magnetic resonance contrast is provided through gadolinium incorporated in the silica matrix, whereas the photoacoustic signal originates from nonuniform, discontinuous gold nanodomains speckled across the silica surface.

View Article: PubMed Central - PubMed

Affiliation: Materials Science and Engineering and Particle Engineering Research Center, Molecular Genetics and Microbiology, Biomedical Engineering, Physiology and Functional Genomics, and Department of Surgery, University of Florida, P.O. Box 116135 Gainesville, Florida 32611, and NanoScience Technology Center, Chemistry and Biomolecular Science Center, University of Central Florida, Orlando, Florida 32826.

ABSTRACT
Multimodal Gold-speckled silica nanoparticles as contrast agents for noninvasive imaging with magnetic resonance imaging and photoacoustic tomography have been prepared in a simple one-pot synthesis using nonionic microemulsions. Magnetic resonance contrast is provided through gadolinium incorporated in the silica matrix, whereas the photoacoustic signal originates from nonuniform, discontinuous gold nanodomains speckled across the silica surface.

No MeSH data available.


(A) Representative TEM picture of Gd-doped GSS nanoparticles, with an average size of ∼100 nm and (B) Gd-doped GSS particles with an average size of ∼50 nm. (C) High-resolution TEM picture of the nanoparticle showing the presence of electron-dense gold on the silica nanoparticle and (D) dark-field TEM picture showing the contrast from the presence of the electron-dense species.
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fig1: (A) Representative TEM picture of Gd-doped GSS nanoparticles, with an average size of ∼100 nm and (B) Gd-doped GSS particles with an average size of ∼50 nm. (C) High-resolution TEM picture of the nanoparticle showing the presence of electron-dense gold on the silica nanoparticle and (D) dark-field TEM picture showing the contrast from the presence of the electron-dense species.

Mentions: The complete synthesis of the multimodal GSS nanoparticles has been carried out in one pot using the nonionic W/O microemulsion method. The synthesis procedure for these nanoparticles has been described in detail in the . Briefly, Gd-doped silica nanoparticles are first formed by cocondensation of TEOS and a silane reagent that strongly chelates polyvalent metal ions30−34 (TSPETE) in the water core of the TX-100/n-hexanol/water W/O microemulsion. Incorporation of chloroauric acid followed by its reduction was the carried out within the surface layer of the silica nanoparticles. By manipulating W0 of the microemulsions and the reactant concentrations, we were able to tune Gd-doped GSS nanoparticle size from less than 50 to 200 nm. Parts A and B of Figure 1 show representative TEM micrographs for two different samples prepared at W0 10 and 14, depicting mean particle size of ∼100 (±10) and 55 (±5) nm, respectively. GSS nanoparticles up to 200 nm and larger were synthesized at W0 5 (Supporting Information) using the same microemulsion system. The particle sizes were confirmed using DLS and disk centrifuge techniques.


Gold-Speckled Multimodal Nanoparticles for Noninvasive Bioimaging.

Sharma P, Brown SC, Bengtsson N, Zhang Q, Walter GA, Grobmyer SR, Santra S, Jiang H, Scott EW, Moudgil BM - Chem Mater (2008)

(A) Representative TEM picture of Gd-doped GSS nanoparticles, with an average size of ∼100 nm and (B) Gd-doped GSS particles with an average size of ∼50 nm. (C) High-resolution TEM picture of the nanoparticle showing the presence of electron-dense gold on the silica nanoparticle and (D) dark-field TEM picture showing the contrast from the presence of the electron-dense species.
© Copyright Policy - open-access - ccc-price
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2664628&req=5

fig1: (A) Representative TEM picture of Gd-doped GSS nanoparticles, with an average size of ∼100 nm and (B) Gd-doped GSS particles with an average size of ∼50 nm. (C) High-resolution TEM picture of the nanoparticle showing the presence of electron-dense gold on the silica nanoparticle and (D) dark-field TEM picture showing the contrast from the presence of the electron-dense species.
Mentions: The complete synthesis of the multimodal GSS nanoparticles has been carried out in one pot using the nonionic W/O microemulsion method. The synthesis procedure for these nanoparticles has been described in detail in the . Briefly, Gd-doped silica nanoparticles are first formed by cocondensation of TEOS and a silane reagent that strongly chelates polyvalent metal ions30−34 (TSPETE) in the water core of the TX-100/n-hexanol/water W/O microemulsion. Incorporation of chloroauric acid followed by its reduction was the carried out within the surface layer of the silica nanoparticles. By manipulating W0 of the microemulsions and the reactant concentrations, we were able to tune Gd-doped GSS nanoparticle size from less than 50 to 200 nm. Parts A and B of Figure 1 show representative TEM micrographs for two different samples prepared at W0 10 and 14, depicting mean particle size of ∼100 (±10) and 55 (±5) nm, respectively. GSS nanoparticles up to 200 nm and larger were synthesized at W0 5 (Supporting Information) using the same microemulsion system. The particle sizes were confirmed using DLS and disk centrifuge techniques.

Bottom Line: Multimodal Gold-speckled silica nanoparticles as contrast agents for noninvasive imaging with magnetic resonance imaging and photoacoustic tomography have been prepared in a simple one-pot synthesis using nonionic microemulsions.Magnetic resonance contrast is provided through gadolinium incorporated in the silica matrix, whereas the photoacoustic signal originates from nonuniform, discontinuous gold nanodomains speckled across the silica surface.

View Article: PubMed Central - PubMed

Affiliation: Materials Science and Engineering and Particle Engineering Research Center, Molecular Genetics and Microbiology, Biomedical Engineering, Physiology and Functional Genomics, and Department of Surgery, University of Florida, P.O. Box 116135 Gainesville, Florida 32611, and NanoScience Technology Center, Chemistry and Biomolecular Science Center, University of Central Florida, Orlando, Florida 32826.

ABSTRACT
Multimodal Gold-speckled silica nanoparticles as contrast agents for noninvasive imaging with magnetic resonance imaging and photoacoustic tomography have been prepared in a simple one-pot synthesis using nonionic microemulsions. Magnetic resonance contrast is provided through gadolinium incorporated in the silica matrix, whereas the photoacoustic signal originates from nonuniform, discontinuous gold nanodomains speckled across the silica surface.

No MeSH data available.