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Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis.

Potu BK, Rao MS, Kutty NG, Bhat KM, Chamallamudi MR, Nayak SR - Clinics (Sao Paulo) (2008)

Bottom Line: The ossified cartilage (bone) was morphometrically analyzed using Scion image software.Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Kasturba Medical College, Manipal University.

ABSTRACT

Objective: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats.

Methods: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software.

Results: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.

Conclusion: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

Show MeSH
Percentages of total lengths of humerus, radius, and ulna ossified in pups born to control and treated (petroleum ether extract of CQ, 500 mg/kg/day) mothers. Note that there are significant increases in the lengths of the ossified humerus, (**** P<0.0001, Student’s t-test), radius, and ulna (*** P<0.001 Student’s t-test) in pups born to CQ-treated mothers
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f1-18-br-0158: Percentages of total lengths of humerus, radius, and ulna ossified in pups born to control and treated (petroleum ether extract of CQ, 500 mg/kg/day) mothers. Note that there are significant increases in the lengths of the ossified humerus, (**** P<0.0001, Student’s t-test), radius, and ulna (*** P<0.001 Student’s t-test) in pups born to CQ-treated mothers

Mentions: The scion image data concerning the length of ossified skeleton revealed a significant difference in the extent of ossification of the humerus in treated compared to control pups. The mean length of the ossified humerus in the control pups was 3.97 ± 0.06 mm (n=6; 64.97 ± 1.09% of total length of the humerus), while the mean length of the ossified humerus in pups born to CQ-treated mothers was 4.36 ± 0.04 mm (n=6;73.84 ± 0.89% of total length of the humerus (P<0.0001, Student’s t-test; Figures 1 and 2).


Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis.

Potu BK, Rao MS, Kutty NG, Bhat KM, Chamallamudi MR, Nayak SR - Clinics (Sao Paulo) (2008)

Percentages of total lengths of humerus, radius, and ulna ossified in pups born to control and treated (petroleum ether extract of CQ, 500 mg/kg/day) mothers. Note that there are significant increases in the lengths of the ossified humerus, (**** P<0.0001, Student’s t-test), radius, and ulna (*** P<0.001 Student’s t-test) in pups born to CQ-treated mothers
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664284&req=5

f1-18-br-0158: Percentages of total lengths of humerus, radius, and ulna ossified in pups born to control and treated (petroleum ether extract of CQ, 500 mg/kg/day) mothers. Note that there are significant increases in the lengths of the ossified humerus, (**** P<0.0001, Student’s t-test), radius, and ulna (*** P<0.001 Student’s t-test) in pups born to CQ-treated mothers
Mentions: The scion image data concerning the length of ossified skeleton revealed a significant difference in the extent of ossification of the humerus in treated compared to control pups. The mean length of the ossified humerus in the control pups was 3.97 ± 0.06 mm (n=6; 64.97 ± 1.09% of total length of the humerus), while the mean length of the ossified humerus in pups born to CQ-treated mothers was 4.36 ± 0.04 mm (n=6;73.84 ± 0.89% of total length of the humerus (P<0.0001, Student’s t-test; Figures 1 and 2).

Bottom Line: The ossified cartilage (bone) was morphometrically analyzed using Scion image software.Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Kasturba Medical College, Manipal University.

ABSTRACT

Objective: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats.

Methods: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software.

Results: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.

Conclusion: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

Show MeSH