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Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens.

Perniconi SE, Simões Mde J, Simões Rdos S, Haidar MA, Baracat EC, Soares JM - Clinics (Sao Paulo) (2008)

Bottom Line: The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01).However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight.Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.

View Article: PubMed Central - PubMed

Affiliation: Morphology Department, UNIFESP, São Paulo, SP, Brazil.

ABSTRACT

Objective: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats.

Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 microg/kg; GE0.5mg, group receiving CEE at 500 microg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program.

Results: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight.

Conclusion: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.

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Photomicrography showing the cortical region of an ovary in 1.25 mm extension. Letters indicate the delimitation applied to histomorphometric parameters. A) tracing along the superficial epithelial cells. B) tracing along the epithelium-stroma interface. Staining: H&E. Magnification: 400X
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f1-cln63_3p0381: Photomicrography showing the cortical region of an ovary in 1.25 mm extension. Letters indicate the delimitation applied to histomorphometric parameters. A) tracing along the superficial epithelial cells. B) tracing along the epithelium-stroma interface. Staining: H&E. Magnification: 400X

Mentions: Morphometric studies were performed using a light microscope (Axiolab, Carl Zeiss) attached to an image capture system (AxionLab, Carl Zeiss). OSE measurements were made at four clock positions (12, 3, 6, and 9 o’clock) on serial sections using the AxionVision Morphometric Program (Carl Zeiss). A total of 40 consecutive observations were recorded for each animal (10 histological sections per rat). Also, the total evaluated width of the epithelium was approximately 5 mm per ovary (sums of all clock positions). The ovarian surface epithelial thickness, area, and perimeter were measured on profiles showing the whole epithelium from basement membrane to surface. The thickness was calculated from the average distances between the epithelium-stroma interface and the cell surface. The perimeter and area were measured by tracing the epithelium-stroma interface (Fig. 1). The area (A) and perimeter (P) formulas are as follows: A = length x width, and P = (2 x length) + (2 x width).


Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens.

Perniconi SE, Simões Mde J, Simões Rdos S, Haidar MA, Baracat EC, Soares JM - Clinics (Sao Paulo) (2008)

Photomicrography showing the cortical region of an ovary in 1.25 mm extension. Letters indicate the delimitation applied to histomorphometric parameters. A) tracing along the superficial epithelial cells. B) tracing along the epithelium-stroma interface. Staining: H&E. Magnification: 400X
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664246&req=5

f1-cln63_3p0381: Photomicrography showing the cortical region of an ovary in 1.25 mm extension. Letters indicate the delimitation applied to histomorphometric parameters. A) tracing along the superficial epithelial cells. B) tracing along the epithelium-stroma interface. Staining: H&E. Magnification: 400X
Mentions: Morphometric studies were performed using a light microscope (Axiolab, Carl Zeiss) attached to an image capture system (AxionLab, Carl Zeiss). OSE measurements were made at four clock positions (12, 3, 6, and 9 o’clock) on serial sections using the AxionVision Morphometric Program (Carl Zeiss). A total of 40 consecutive observations were recorded for each animal (10 histological sections per rat). Also, the total evaluated width of the epithelium was approximately 5 mm per ovary (sums of all clock positions). The ovarian surface epithelial thickness, area, and perimeter were measured on profiles showing the whole epithelium from basement membrane to surface. The thickness was calculated from the average distances between the epithelium-stroma interface and the cell surface. The perimeter and area were measured by tracing the epithelium-stroma interface (Fig. 1). The area (A) and perimeter (P) formulas are as follows: A = length x width, and P = (2 x length) + (2 x width).

Bottom Line: The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01).However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight.Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.

View Article: PubMed Central - PubMed

Affiliation: Morphology Department, UNIFESP, São Paulo, SP, Brazil.

ABSTRACT

Objective: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats.

Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 microg/kg; GE0.5mg, group receiving CEE at 500 microg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program.

Results: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight.

Conclusion: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.

Show MeSH