Limits...
A study of the antioxidant effect of alpha lipoic acids on sperm quality.

Ibrahim SF, Osman K, Das S, Othman AM, Majid NA, Rahman MP - Clinics (Sao Paulo) (2008)

Bottom Line: The comet assay was used to observe the effect of Alpha lipoic acids on sperm DNA integrity.This concentration was also capable of reducing DNA damage.In conclusion, Alpha lipoic acids renders cryoprotection to sperm, thereby improving sperm quality.

View Article: PubMed Central - PubMed

Affiliation: Universiti Kebangsaan, Physiology, Fakulti Perubatan, Malaysia. timi@medic.ukm.my

ABSTRACT

Objective: Assisted reproductive techniques are useful in helping infertile couples achieve successful conception. Initial studies have shown that sperm cryopreservation, one step in assisted reproduction, causes a dramatic reduction in sperm quality. This has been attributed to, among other things, free radical activities. The aim of the present study was to minimize this oxidative attack by adding an antioxidant into the sperm microenvironment. Alpha lipoic acids were selected for this purpose for their efficient free radical scavenging properties and solubility in lipid and aqueous phases.

Methods: For this investigation, semen from six Boer bucks was pooled. Seminal analysis of the baseline prior to incubation of samples with different concentrations of Alpha lipoic acids (0.00625, 0.0125, 0.025, 0.05, 0.1 mmol/ml) was performed, and post-seminal analysis was conducted after a one-hour incubation. The comet assay was used to observe the effect of Alpha lipoic acids on sperm DNA integrity. Statistical analysis using an unpaired t-test with a significance level of p<0.05 was then performed.

Results: Our results indicate that the sperm motility rate was improved after incubation with Alpha lipoic acids at a concentration of 0.02 mmol/ml. This concentration was also capable of reducing DNA damage.

Conclusion: In conclusion, Alpha lipoic acids renders cryoprotection to sperm, thereby improving sperm quality.

Show MeSH

Related in: MedlinePlus

Dose response curve of ALA against sperm motility. Sperm motility was assessed using light microscope with Mtrack J Imaging System on the Weber sterility chamber. Mann-Whitney t-test was used to determine the differences between baseline (pre) and after one-hour incubation (post). r2 = 0.0649 *P<0.05. N=31
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2664134&req=5

f1-cln63_4p0545: Dose response curve of ALA against sperm motility. Sperm motility was assessed using light microscope with Mtrack J Imaging System on the Weber sterility chamber. Mann-Whitney t-test was used to determine the differences between baseline (pre) and after one-hour incubation (post). r2 = 0.0649 *P<0.05. N=31

Mentions: There was a significant change in sperm motility in all concentration used. However, the percentages of changes were different for each of the concentration groups. The percentage of motile sperm increased dramatically, from 112.8% (concentration of 0.00625 mmol/ml) to 251.0% (0.0125 mmol/ml). The increasing phase pattern change reversed following introduction of more concentrated solutions of ALA. This is clearly shown in the graph in Figure 1.


A study of the antioxidant effect of alpha lipoic acids on sperm quality.

Ibrahim SF, Osman K, Das S, Othman AM, Majid NA, Rahman MP - Clinics (Sao Paulo) (2008)

Dose response curve of ALA against sperm motility. Sperm motility was assessed using light microscope with Mtrack J Imaging System on the Weber sterility chamber. Mann-Whitney t-test was used to determine the differences between baseline (pre) and after one-hour incubation (post). r2 = 0.0649 *P<0.05. N=31
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2664134&req=5

f1-cln63_4p0545: Dose response curve of ALA against sperm motility. Sperm motility was assessed using light microscope with Mtrack J Imaging System on the Weber sterility chamber. Mann-Whitney t-test was used to determine the differences between baseline (pre) and after one-hour incubation (post). r2 = 0.0649 *P<0.05. N=31
Mentions: There was a significant change in sperm motility in all concentration used. However, the percentages of changes were different for each of the concentration groups. The percentage of motile sperm increased dramatically, from 112.8% (concentration of 0.00625 mmol/ml) to 251.0% (0.0125 mmol/ml). The increasing phase pattern change reversed following introduction of more concentrated solutions of ALA. This is clearly shown in the graph in Figure 1.

Bottom Line: The comet assay was used to observe the effect of Alpha lipoic acids on sperm DNA integrity.This concentration was also capable of reducing DNA damage.In conclusion, Alpha lipoic acids renders cryoprotection to sperm, thereby improving sperm quality.

View Article: PubMed Central - PubMed

Affiliation: Universiti Kebangsaan, Physiology, Fakulti Perubatan, Malaysia. timi@medic.ukm.my

ABSTRACT

Objective: Assisted reproductive techniques are useful in helping infertile couples achieve successful conception. Initial studies have shown that sperm cryopreservation, one step in assisted reproduction, causes a dramatic reduction in sperm quality. This has been attributed to, among other things, free radical activities. The aim of the present study was to minimize this oxidative attack by adding an antioxidant into the sperm microenvironment. Alpha lipoic acids were selected for this purpose for their efficient free radical scavenging properties and solubility in lipid and aqueous phases.

Methods: For this investigation, semen from six Boer bucks was pooled. Seminal analysis of the baseline prior to incubation of samples with different concentrations of Alpha lipoic acids (0.00625, 0.0125, 0.025, 0.05, 0.1 mmol/ml) was performed, and post-seminal analysis was conducted after a one-hour incubation. The comet assay was used to observe the effect of Alpha lipoic acids on sperm DNA integrity. Statistical analysis using an unpaired t-test with a significance level of p<0.05 was then performed.

Results: Our results indicate that the sperm motility rate was improved after incubation with Alpha lipoic acids at a concentration of 0.02 mmol/ml. This concentration was also capable of reducing DNA damage.

Conclusion: In conclusion, Alpha lipoic acids renders cryoprotection to sperm, thereby improving sperm quality.

Show MeSH
Related in: MedlinePlus