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Cellular liver regeneration after extended hepatic resection in pigs.

Ladurner R, Traub F, Schenk M, Königsrainer A, Glatzle J - HPB Surg (2009)

Bottom Line: During follow up, liver regeneration was estimated by macroscopic markers such as liver volume and size of the portal fields [mm(2)] as well as the amount of hepatocytes per portal field and the amount of hepatocytes per mm(2).The size of the portal fields increased significantly as well as the number of hepatocytes in the portal fields.Interestingly, the number of hepatocytes per mm(2) remained the same.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Visceral and Transplant Surgery, University Hospital Tübingen, Hoppe-Seyler-Street 3, D-72076 Tübingen, Germany. ruth.ladurner@med.uni-tuebingen.de

ABSTRACT

Background: The liver has an enormous capacity to regenerate itself. The aim of this study was to evaluate whether the regeneration is due to hypertrophy or hyperplasia of the remnant liver after extended resection and whether a portosystemic shunt is beneficial.

Material and methods: An extended left hemihepatectomy was performed in 25 pigs, and in 14 after performing a portosystemic shunt. During follow up, liver regeneration was estimated by macroscopic markers such as liver volume and size of the portal fields [mm(2)] as well as the amount of hepatocytes per portal field and the amount of hepatocytes per mm(2).

Results: Regardless of the operation procedure, the volume of the remnant liver increased about 2.5 fold at the end of the first week after resection. The size of the portal fields increased significantly as well as the number of hepatocytes in the portal fields. Interestingly, the number of hepatocytes per mm(2) remained the same.

Conclusion: After extended resection, liver regeneration was achieved by an extensive and significant hyperplasia of hepatocytes within the preexisting portal fields and not by de novo synthesis of new portal fields. However, there was no difference in liver regeneration regarding the operation procedure performed with or without portosystemic shunt.

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Related in: MedlinePlus

The density of hepatocytes per mm2 is expressed in Figure 3 in animals operated without (Figures 3(a) and 3(c)) and with portosystemic H-shunt (Figures 3(b) and 3(d)). The density of hepatocytes is shown in relation to the postoperative time (weeks) and is expressed as mean  ± SEM in Figures 3(a) and 3(b) and as correlation coefficient in Figures 3(c) and 3(d).
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fig3: The density of hepatocytes per mm2 is expressed in Figure 3 in animals operated without (Figures 3(a) and 3(c)) and with portosystemic H-shunt (Figures 3(b) and 3(d)). The density of hepatocytes is shown in relation to the postoperative time (weeks) and is expressed as mean ± SEM in Figures 3(a) and 3(b) and as correlation coefficient in Figures 3(c) and 3(d).

Mentions: The number of hepatocytes within the portal fields increased significantly over time, at approximately 2.6 fold within 3 weeks, regardless of the operation procedure with or without portosystemic shunt. The correlation coefficient was in both groups high, measuring r2 = 0.935 and r2 = 0.909 in pigs resected with or without shunt, respectively, (hepatocytes/portal field, animals with shunt before resection: 3438 ± 1281; 1 week after resection: 5831 ± 3419*; 3 weeks: 8793 ± 3690*, *P < .05; animals without shunt before resection: 3053 ± 1096; 1 week after resection: 4580 ± 2007*; 3 weeks: 8014 ± 4809*, *P < .05).


Cellular liver regeneration after extended hepatic resection in pigs.

Ladurner R, Traub F, Schenk M, Königsrainer A, Glatzle J - HPB Surg (2009)

The density of hepatocytes per mm2 is expressed in Figure 3 in animals operated without (Figures 3(a) and 3(c)) and with portosystemic H-shunt (Figures 3(b) and 3(d)). The density of hepatocytes is shown in relation to the postoperative time (weeks) and is expressed as mean  ± SEM in Figures 3(a) and 3(b) and as correlation coefficient in Figures 3(c) and 3(d).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2663376&req=5

fig3: The density of hepatocytes per mm2 is expressed in Figure 3 in animals operated without (Figures 3(a) and 3(c)) and with portosystemic H-shunt (Figures 3(b) and 3(d)). The density of hepatocytes is shown in relation to the postoperative time (weeks) and is expressed as mean ± SEM in Figures 3(a) and 3(b) and as correlation coefficient in Figures 3(c) and 3(d).
Mentions: The number of hepatocytes within the portal fields increased significantly over time, at approximately 2.6 fold within 3 weeks, regardless of the operation procedure with or without portosystemic shunt. The correlation coefficient was in both groups high, measuring r2 = 0.935 and r2 = 0.909 in pigs resected with or without shunt, respectively, (hepatocytes/portal field, animals with shunt before resection: 3438 ± 1281; 1 week after resection: 5831 ± 3419*; 3 weeks: 8793 ± 3690*, *P < .05; animals without shunt before resection: 3053 ± 1096; 1 week after resection: 4580 ± 2007*; 3 weeks: 8014 ± 4809*, *P < .05).

Bottom Line: During follow up, liver regeneration was estimated by macroscopic markers such as liver volume and size of the portal fields [mm(2)] as well as the amount of hepatocytes per portal field and the amount of hepatocytes per mm(2).The size of the portal fields increased significantly as well as the number of hepatocytes in the portal fields.Interestingly, the number of hepatocytes per mm(2) remained the same.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Visceral and Transplant Surgery, University Hospital Tübingen, Hoppe-Seyler-Street 3, D-72076 Tübingen, Germany. ruth.ladurner@med.uni-tuebingen.de

ABSTRACT

Background: The liver has an enormous capacity to regenerate itself. The aim of this study was to evaluate whether the regeneration is due to hypertrophy or hyperplasia of the remnant liver after extended resection and whether a portosystemic shunt is beneficial.

Material and methods: An extended left hemihepatectomy was performed in 25 pigs, and in 14 after performing a portosystemic shunt. During follow up, liver regeneration was estimated by macroscopic markers such as liver volume and size of the portal fields [mm(2)] as well as the amount of hepatocytes per portal field and the amount of hepatocytes per mm(2).

Results: Regardless of the operation procedure, the volume of the remnant liver increased about 2.5 fold at the end of the first week after resection. The size of the portal fields increased significantly as well as the number of hepatocytes in the portal fields. Interestingly, the number of hepatocytes per mm(2) remained the same.

Conclusion: After extended resection, liver regeneration was achieved by an extensive and significant hyperplasia of hepatocytes within the preexisting portal fields and not by de novo synthesis of new portal fields. However, there was no difference in liver regeneration regarding the operation procedure performed with or without portosystemic shunt.

Show MeSH
Related in: MedlinePlus