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Negative Smad expression and regulation in the developing chick limb.

Vargesson N, Laufer E - PLoS ONE (2009)

Bottom Line: The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery.Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb.Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Development, Columbia University, New York, New York, United States of America.

ABSTRACT
The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery. We report the cloning of one Smad6 and two Smad7 (Smad7a and Smad7b) chick homologs and their expression and regulation in the developing limb. Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb. Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

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Related in: MedlinePlus

cSmad6 is expressed in limb mesenchyme, epidermis and cartilage.Expression of cSmad6 mRNA in longitudinal (A–C, F–H) and transverse (D, F, I) sections of HH st29 forelimbs (A–E) and hindlimbs (F–I). B, C, G, H are higher power images of labeled areas in A and F. Arrows indicate expression of cSmad6 in interdigital mesenchyme (black arrow); hypertrophic chondrocytes (blue arrow); perichondrium/mesenchyme adjacent to cartilage (magenta arrow); nascent joints (purple arrow); interstitial mesenchyme (yellow arrow); subepidermal mesenchyme (white arrow); epidermis (green arrow). Scale bars indicate 100 µm.
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pone-0005173-g003: cSmad6 is expressed in limb mesenchyme, epidermis and cartilage.Expression of cSmad6 mRNA in longitudinal (A–C, F–H) and transverse (D, F, I) sections of HH st29 forelimbs (A–E) and hindlimbs (F–I). B, C, G, H are higher power images of labeled areas in A and F. Arrows indicate expression of cSmad6 in interdigital mesenchyme (black arrow); hypertrophic chondrocytes (blue arrow); perichondrium/mesenchyme adjacent to cartilage (magenta arrow); nascent joints (purple arrow); interstitial mesenchyme (yellow arrow); subepidermal mesenchyme (white arrow); epidermis (green arrow). Scale bars indicate 100 µm.

Mentions: We also examined cSmad6 expression by section in situ hybridization on HH st29 forelimb (Figure 3A–E) and hindlimb (Figure 3F–I) cryosectioned tissue. We detected expression in interdigital mesenchyme (Figure 3A,F,I), perichrondrium (Figure 3B,D,F), hypertrophic chondrocytes (Figure 3A,B), forming joints (Figure 3A,F–H), interstitial mesenchyme (Figure 3A,F,G), subepidermal mesenchyme (Figure 3A,D,E) and epidermis (Figure 3F,G).


Negative Smad expression and regulation in the developing chick limb.

Vargesson N, Laufer E - PLoS ONE (2009)

cSmad6 is expressed in limb mesenchyme, epidermis and cartilage.Expression of cSmad6 mRNA in longitudinal (A–C, F–H) and transverse (D, F, I) sections of HH st29 forelimbs (A–E) and hindlimbs (F–I). B, C, G, H are higher power images of labeled areas in A and F. Arrows indicate expression of cSmad6 in interdigital mesenchyme (black arrow); hypertrophic chondrocytes (blue arrow); perichondrium/mesenchyme adjacent to cartilage (magenta arrow); nascent joints (purple arrow); interstitial mesenchyme (yellow arrow); subepidermal mesenchyme (white arrow); epidermis (green arrow). Scale bars indicate 100 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2663054&req=5

pone-0005173-g003: cSmad6 is expressed in limb mesenchyme, epidermis and cartilage.Expression of cSmad6 mRNA in longitudinal (A–C, F–H) and transverse (D, F, I) sections of HH st29 forelimbs (A–E) and hindlimbs (F–I). B, C, G, H are higher power images of labeled areas in A and F. Arrows indicate expression of cSmad6 in interdigital mesenchyme (black arrow); hypertrophic chondrocytes (blue arrow); perichondrium/mesenchyme adjacent to cartilage (magenta arrow); nascent joints (purple arrow); interstitial mesenchyme (yellow arrow); subepidermal mesenchyme (white arrow); epidermis (green arrow). Scale bars indicate 100 µm.
Mentions: We also examined cSmad6 expression by section in situ hybridization on HH st29 forelimb (Figure 3A–E) and hindlimb (Figure 3F–I) cryosectioned tissue. We detected expression in interdigital mesenchyme (Figure 3A,F,I), perichrondrium (Figure 3B,D,F), hypertrophic chondrocytes (Figure 3A,B), forming joints (Figure 3A,F–H), interstitial mesenchyme (Figure 3A,F,G), subepidermal mesenchyme (Figure 3A,D,E) and epidermis (Figure 3F,G).

Bottom Line: The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery.Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb.Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Development, Columbia University, New York, New York, United States of America.

ABSTRACT
The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery. We report the cloning of one Smad6 and two Smad7 (Smad7a and Smad7b) chick homologs and their expression and regulation in the developing limb. Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb. Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

Show MeSH
Related in: MedlinePlus