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Human parainfluenza virus type 2 (HPIV2) induced host ADAM8 expression in human salivary adenocarcinoma cell line (HSY) during cell fusion.

Ma GF, Miettinen S, Porola P, Hedman K, Salo J, Konttinen YT - BMC Microbiol. (2009)

Bottom Line: Without HPIV2 the corresponding percentages were only 7.7% and 8.8%.Moreover, ADAM8 positive cells formed bi- (16.2%) and multinuclear cells (3.5%) on day one and the corresponding percentages on day three were 15.6% for binuclear and 57.2% for multinuclear cells.The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.

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Affiliation: Department of Medicine/invärtes medicin, Helsinki University Central Hospital, PO Box 700, Helsinki, Finland. guofeng.ma@helsinki.fi

ABSTRACT

Background: The aim of the study was to investigate expression of ADAMs (A Disintegrin and A Metalloproteinase) of host cell origin during cell-cell fusion induced by human parainfluenza virus type 2 (HPIV2).

Results: Induction of host cell ADAM9 was observed in GMK cells, but the applicability of this model was restricted by lack of cross-reactivity of the anti-human ADAM8 antibodies with the corresponding green monkey antigens. HSG cells were not susceptible to HPIV2 virus infection. In contrast, in human parotid gland HSY cells, a natural host cell for paramyxoviruses, HPIV2 induced ADAM8 expression. ADAM8 staining increased dramatically over time from 7.9 +/- 3% at zero hours to 99.2 +/- 0.8% at 72 hours (p = 0.0001). Without HPIV2 the corresponding percentages were only 7.7% and 8.8%. Moreover, ADAM8 positive cells formed bi- (16.2%) and multinuclear cells (3.5%) on day one and the corresponding percentages on day three were 15.6% for binuclear and 57.2% for multinuclear cells.

Conclusion: ADAM8, well recognized for participation in cell-to-cell fusion especially in osteoclast formation, is up-regulated upon formation of multinuclear giant cells after HPIV2 induction in HSY cells. The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.

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The proportion of mononuclear (black square), binuclear (black upwards pointing triangle) and multinuclear positive cells (downwards pointing triangle) of all ADAM8 positive cells in the immunofluorescence staining of ADAM8 in HPIV2 stimulated human salivary adenocarcinoma cell cultures on culture days 0, 1 and 3 as a function of time.
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Figure 3: The proportion of mononuclear (black square), binuclear (black upwards pointing triangle) and multinuclear positive cells (downwards pointing triangle) of all ADAM8 positive cells in the immunofluorescence staining of ADAM8 in HPIV2 stimulated human salivary adenocarcinoma cell cultures on culture days 0, 1 and 3 as a function of time.


Human parainfluenza virus type 2 (HPIV2) induced host ADAM8 expression in human salivary adenocarcinoma cell line (HSY) during cell fusion.

Ma GF, Miettinen S, Porola P, Hedman K, Salo J, Konttinen YT - BMC Microbiol. (2009)

The proportion of mononuclear (black square), binuclear (black upwards pointing triangle) and multinuclear positive cells (downwards pointing triangle) of all ADAM8 positive cells in the immunofluorescence staining of ADAM8 in HPIV2 stimulated human salivary adenocarcinoma cell cultures on culture days 0, 1 and 3 as a function of time.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662866&req=5

Figure 3: The proportion of mononuclear (black square), binuclear (black upwards pointing triangle) and multinuclear positive cells (downwards pointing triangle) of all ADAM8 positive cells in the immunofluorescence staining of ADAM8 in HPIV2 stimulated human salivary adenocarcinoma cell cultures on culture days 0, 1 and 3 as a function of time.
Bottom Line: Without HPIV2 the corresponding percentages were only 7.7% and 8.8%.Moreover, ADAM8 positive cells formed bi- (16.2%) and multinuclear cells (3.5%) on day one and the corresponding percentages on day three were 15.6% for binuclear and 57.2% for multinuclear cells.The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Medicine/invärtes medicin, Helsinki University Central Hospital, PO Box 700, Helsinki, Finland. guofeng.ma@helsinki.fi

ABSTRACT

Background: The aim of the study was to investigate expression of ADAMs (A Disintegrin and A Metalloproteinase) of host cell origin during cell-cell fusion induced by human parainfluenza virus type 2 (HPIV2).

Results: Induction of host cell ADAM9 was observed in GMK cells, but the applicability of this model was restricted by lack of cross-reactivity of the anti-human ADAM8 antibodies with the corresponding green monkey antigens. HSG cells were not susceptible to HPIV2 virus infection. In contrast, in human parotid gland HSY cells, a natural host cell for paramyxoviruses, HPIV2 induced ADAM8 expression. ADAM8 staining increased dramatically over time from 7.9 +/- 3% at zero hours to 99.2 +/- 0.8% at 72 hours (p = 0.0001). Without HPIV2 the corresponding percentages were only 7.7% and 8.8%. Moreover, ADAM8 positive cells formed bi- (16.2%) and multinuclear cells (3.5%) on day one and the corresponding percentages on day three were 15.6% for binuclear and 57.2% for multinuclear cells.

Conclusion: ADAM8, well recognized for participation in cell-to-cell fusion especially in osteoclast formation, is up-regulated upon formation of multinuclear giant cells after HPIV2 induction in HSY cells. The virus-HSY cell system provides a novel experimental model for study of the molecular mechanism of cell fusion events.

Show MeSH
Related in: MedlinePlus