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Cell proliferation in the Drosophila adult brain revealed by clonal analysis and bromodeoxyuridine labelling.

von Trotha JW, Egger B, Brand AH - Neural Dev (2009)

Bottom Line: Of the BrdU-labelled cells, 86% express the glial gene reversed polarity (repo), and 14% are repo negative.We have observed cell proliferation in the Drosophila adult brain.The dividing cells may be adult stem cells, generating glial and/or non-glial cell types.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Wellcome Trust/Cancer Research UK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK. j.v.trotha@gurdon.cam.ac.uk

ABSTRACT

Background: The production of new neurons during adulthood and their subsequent integration into a mature central nervous system have been shown to occur in all vertebrate species examined to date. However, the situation in insects is less clear and, in particular, it has been reported that there is no proliferation in the Drosophila adult brain.

Results: We report here, using clonal analysis and 5'-bromo-2'-deoxyuridine (BrdU) labelling, that cell proliferation does occur in the Drosophila adult brain. The majority of clones cluster on the ventrolateral side of the antennal lobes, as do the BrdU-positive cells. Of the BrdU-labelled cells, 86% express the glial gene reversed polarity (repo), and 14% are repo negative.

Conclusion: We have observed cell proliferation in the Drosophila adult brain. The dividing cells may be adult stem cells, generating glial and/or non-glial cell types.

Show MeSH
Mitotic clones around the antennal nerve label with 5'-bromo-2'-deoxyuridine (BrdU). Two mitotic, single cell clones are marked by expression of lacZ in a 3-day-old adult X15-29/X15-33 brain. One of the two β-galactosidase-positive cells also stains for BrdU (white arrow), while the second does not (white arrowhead). The images are a z-series projection of 24 0.3 μm sections. Dorsal is to the upper left. AL, antennal lobe; AN, antennal nerve. The scale bar is 10 μm.
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Figure 5: Mitotic clones around the antennal nerve label with 5'-bromo-2'-deoxyuridine (BrdU). Two mitotic, single cell clones are marked by expression of lacZ in a 3-day-old adult X15-29/X15-33 brain. One of the two β-galactosidase-positive cells also stains for BrdU (white arrow), while the second does not (white arrowhead). The images are a z-series projection of 24 0.3 μm sections. Dorsal is to the upper left. AL, antennal lobe; AN, antennal nerve. The scale bar is 10 μm.

Mentions: To detemine whether the BrdU-labelled cells corresponded directly to the dividing cells we observed by MARCM, we combined BrdU labelling with clonal analysis. In order to avoid the high background of clones we observed in the control MARCM samples, we used a different lineage tracing technique that gives few, if any, background clones [31,42]. In 2 out of 18 BrdU-positive brains we observed cell clones (marked by nuclear β-galactosidase) labelled with BrdU around the antennal nerve (Figure 5). We conclude that cells in the adult brain are actively dividing, and that the majority of these cells express Repo.


Cell proliferation in the Drosophila adult brain revealed by clonal analysis and bromodeoxyuridine labelling.

von Trotha JW, Egger B, Brand AH - Neural Dev (2009)

Mitotic clones around the antennal nerve label with 5'-bromo-2'-deoxyuridine (BrdU). Two mitotic, single cell clones are marked by expression of lacZ in a 3-day-old adult X15-29/X15-33 brain. One of the two β-galactosidase-positive cells also stains for BrdU (white arrow), while the second does not (white arrowhead). The images are a z-series projection of 24 0.3 μm sections. Dorsal is to the upper left. AL, antennal lobe; AN, antennal nerve. The scale bar is 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662830&req=5

Figure 5: Mitotic clones around the antennal nerve label with 5'-bromo-2'-deoxyuridine (BrdU). Two mitotic, single cell clones are marked by expression of lacZ in a 3-day-old adult X15-29/X15-33 brain. One of the two β-galactosidase-positive cells also stains for BrdU (white arrow), while the second does not (white arrowhead). The images are a z-series projection of 24 0.3 μm sections. Dorsal is to the upper left. AL, antennal lobe; AN, antennal nerve. The scale bar is 10 μm.
Mentions: To detemine whether the BrdU-labelled cells corresponded directly to the dividing cells we observed by MARCM, we combined BrdU labelling with clonal analysis. In order to avoid the high background of clones we observed in the control MARCM samples, we used a different lineage tracing technique that gives few, if any, background clones [31,42]. In 2 out of 18 BrdU-positive brains we observed cell clones (marked by nuclear β-galactosidase) labelled with BrdU around the antennal nerve (Figure 5). We conclude that cells in the adult brain are actively dividing, and that the majority of these cells express Repo.

Bottom Line: Of the BrdU-labelled cells, 86% express the glial gene reversed polarity (repo), and 14% are repo negative.We have observed cell proliferation in the Drosophila adult brain.The dividing cells may be adult stem cells, generating glial and/or non-glial cell types.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Wellcome Trust/Cancer Research UK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK. j.v.trotha@gurdon.cam.ac.uk

ABSTRACT

Background: The production of new neurons during adulthood and their subsequent integration into a mature central nervous system have been shown to occur in all vertebrate species examined to date. However, the situation in insects is less clear and, in particular, it has been reported that there is no proliferation in the Drosophila adult brain.

Results: We report here, using clonal analysis and 5'-bromo-2'-deoxyuridine (BrdU) labelling, that cell proliferation does occur in the Drosophila adult brain. The majority of clones cluster on the ventrolateral side of the antennal lobes, as do the BrdU-positive cells. Of the BrdU-labelled cells, 86% express the glial gene reversed polarity (repo), and 14% are repo negative.

Conclusion: We have observed cell proliferation in the Drosophila adult brain. The dividing cells may be adult stem cells, generating glial and/or non-glial cell types.

Show MeSH