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The properties of bioengineered chondrocyte sheets for cartilage regeneration.

Mitani G, Sato M, Lee JI, Kaneshiro N, Ishihara M, Ota N, Kokubo M, Sakai H, Kikuchi T, Mochida J - BMC Biotechnol. (2009)

Bottom Line: The expression of SOX 9, collagen type 2, 27, integrin alpha 10, and fibronectin genes in triple-layered chondrocyte sheets was significantly increased in comparison to those in conventional monolayer culture and in a single chondrocyte sheet, implying a nature similar to ordinary cartilage.In addition, immunohistochemistry demonstrated that collagen type II, fibronectin, and integrin alpha 10 were present in the triple-layered chondrocyte sheets.The results of this study indicate that these chondrocyte sheets with a consistent cartilaginous phenotype and adhesive properties may lead to a new strategy for cartilage regeneration.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Kanagawa, Japan. genya@syd.odn.ne.jp

ABSTRACT

Background: Although the clinical results of autologous chondrocyte implantation for articular cartilage defects have recently improved as a result of advanced techniques based on tissue engineering procedures, problems with cell handling and scaffold imperfections remain to be solved. A new cell-sheet technique has been developed, and is potentially able to overcome these obstacles. Chondrocyte sheets applicable to cartilage regeneration can be prepared with this cell-sheet technique using temperature-responsive culture dishes. However, for clinical application, it is necessary to evaluate the characteristics of the cells in these sheets and to identify their similarities to naive cartilage.

Results: The expression of SOX 9, collagen type 2, 27, integrin alpha 10, and fibronectin genes in triple-layered chondrocyte sheets was significantly increased in comparison to those in conventional monolayer culture and in a single chondrocyte sheet, implying a nature similar to ordinary cartilage. In addition, immunohistochemistry demonstrated that collagen type II, fibronectin, and integrin alpha 10 were present in the triple-layered chondrocyte sheets.

Conclusion: The results of this study indicate that these chondrocyte sheets with a consistent cartilaginous phenotype and adhesive properties may lead to a new strategy for cartilage regeneration.

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Schematic illustration of the distribution pattern of extracellular adhesion molecules in triple-layered chondrocyte sheets. Fibronectin was located peripherally in the triple-layered chondrocyte sheet and integrin α10 was observed close to the chondrocytes, in the pericellular matrix, in the layered construct. However, collagen type II was scattered diffusely throughout the layered cell sheets.
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Figure 5: Schematic illustration of the distribution pattern of extracellular adhesion molecules in triple-layered chondrocyte sheets. Fibronectin was located peripherally in the triple-layered chondrocyte sheet and integrin α10 was observed close to the chondrocytes, in the pericellular matrix, in the layered construct. However, collagen type II was scattered diffusely throughout the layered cell sheets.

Mentions: Immunohistochemical examination revealed that fibronectin, integrin α10, and COL2 were present in the triple-layered chondrocyte cell sheet (Fig. 4). Interestingly, fibronectin was located in the periphery of the triple-layered chondrocyte sheets. (Fig. 4A, D) and COL2 was observed in the pericellular matrix of the triple-layered chondrocyte sheets (Fig. 4B, E). However, in contrast to these two proteins, integrin α10 was diffusely distributed throughout the triple-layered chondrocyte sheets (Fig. 4C, F). These different immunohistochemical features of target proteins are illustrated in Fig. 5, where the main results are presented together.


The properties of bioengineered chondrocyte sheets for cartilage regeneration.

Mitani G, Sato M, Lee JI, Kaneshiro N, Ishihara M, Ota N, Kokubo M, Sakai H, Kikuchi T, Mochida J - BMC Biotechnol. (2009)

Schematic illustration of the distribution pattern of extracellular adhesion molecules in triple-layered chondrocyte sheets. Fibronectin was located peripherally in the triple-layered chondrocyte sheet and integrin α10 was observed close to the chondrocytes, in the pericellular matrix, in the layered construct. However, collagen type II was scattered diffusely throughout the layered cell sheets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662823&req=5

Figure 5: Schematic illustration of the distribution pattern of extracellular adhesion molecules in triple-layered chondrocyte sheets. Fibronectin was located peripherally in the triple-layered chondrocyte sheet and integrin α10 was observed close to the chondrocytes, in the pericellular matrix, in the layered construct. However, collagen type II was scattered diffusely throughout the layered cell sheets.
Mentions: Immunohistochemical examination revealed that fibronectin, integrin α10, and COL2 were present in the triple-layered chondrocyte cell sheet (Fig. 4). Interestingly, fibronectin was located in the periphery of the triple-layered chondrocyte sheets. (Fig. 4A, D) and COL2 was observed in the pericellular matrix of the triple-layered chondrocyte sheets (Fig. 4B, E). However, in contrast to these two proteins, integrin α10 was diffusely distributed throughout the triple-layered chondrocyte sheets (Fig. 4C, F). These different immunohistochemical features of target proteins are illustrated in Fig. 5, where the main results are presented together.

Bottom Line: The expression of SOX 9, collagen type 2, 27, integrin alpha 10, and fibronectin genes in triple-layered chondrocyte sheets was significantly increased in comparison to those in conventional monolayer culture and in a single chondrocyte sheet, implying a nature similar to ordinary cartilage.In addition, immunohistochemistry demonstrated that collagen type II, fibronectin, and integrin alpha 10 were present in the triple-layered chondrocyte sheets.The results of this study indicate that these chondrocyte sheets with a consistent cartilaginous phenotype and adhesive properties may lead to a new strategy for cartilage regeneration.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Kanagawa, Japan. genya@syd.odn.ne.jp

ABSTRACT

Background: Although the clinical results of autologous chondrocyte implantation for articular cartilage defects have recently improved as a result of advanced techniques based on tissue engineering procedures, problems with cell handling and scaffold imperfections remain to be solved. A new cell-sheet technique has been developed, and is potentially able to overcome these obstacles. Chondrocyte sheets applicable to cartilage regeneration can be prepared with this cell-sheet technique using temperature-responsive culture dishes. However, for clinical application, it is necessary to evaluate the characteristics of the cells in these sheets and to identify their similarities to naive cartilage.

Results: The expression of SOX 9, collagen type 2, 27, integrin alpha 10, and fibronectin genes in triple-layered chondrocyte sheets was significantly increased in comparison to those in conventional monolayer culture and in a single chondrocyte sheet, implying a nature similar to ordinary cartilage. In addition, immunohistochemistry demonstrated that collagen type II, fibronectin, and integrin alpha 10 were present in the triple-layered chondrocyte sheets.

Conclusion: The results of this study indicate that these chondrocyte sheets with a consistent cartilaginous phenotype and adhesive properties may lead to a new strategy for cartilage regeneration.

Show MeSH
Related in: MedlinePlus