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Local increase in trapezius muscle oxygenation during and after acupuncture.

Ohkubo M, Hamaoka T, Niwayama M, Murase N, Osada T, Kime R, Kurosawa Y, Sakamoto A, Katsumura T - Dyn Med (2009)

Bottom Line: Two sets of near-infrared spectrometer (NIRS) probes, with 40-mm light-source detector spacing, were placed on the right trapezius muscle, with a 50-mm distance between the probes.Nine healthy acupuncture-experienced subjects were chosen as the experimental (AS) group, and 10 healthy acupuncture-experienced subjects were chosen for the control (no AS) group.In conclusion, oxygenation and blood volume increased, indicating elevated blood flow to the small vessels, not in the distant region used in this study, but in the stimulation region of the trapezius muscle during and after a 2-min AS.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Institute of Fitness and Sports in Kanoya, 1 Shiromizu, Kanoya, Kagoshima, Japan. ohkubo@kuretake.ac.jp

ABSTRACT

Purpose: This study aimed to compare the trapezius muscle blood volume and oxygenation in the stimulation region and in a distant region in the same muscle during acupuncture stimulation (AS). We hypothesized that AS provokes a localized increase in muscle blood volume and oxygenation in the stimulation region.

Methods: Two sets of near-infrared spectrometer (NIRS) probes, with 40-mm light-source detector spacing, were placed on the right trapezius muscle, with a 50-mm distance between the probes. Changes in muscle oxygenation (oxy-Hb) and blood volume (t-Hb) in stimulation and distant regions (50 mm away from the stimulation point) were measured using NIRS. Nine healthy acupuncture-experienced subjects were chosen as the experimental (AS) group, and 10 healthy acupuncture-experienced subjects were chosen for the control (no AS) group. Measurements began with a 3-min rest period, followed by "Jakutaku" (AS) for 2 min, and recovery after stimulation.

Results: There was a significant increase in oxy-Hb (60.7 muM at maximum) and t-Hb (48.1 muM at maximum) in the stimulation region compared to the distant region. In the stimulation region, a significant increase in oxy-Hb and t-Hb compared with the pre-stimulation level was first noted at 58.5 s and 13.5 s, respectively, after the onset of stimulation.

Conclusion: In conclusion, oxygenation and blood volume increased, indicating elevated blood flow to the small vessels, not in the distant region used in this study, but in the stimulation region of the trapezius muscle during and after a 2-min AS.

No MeSH data available.


Measurement site and stimulation point (a) and measurement area and stimulation point (b).
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Figure 1: Measurement site and stimulation point (a) and measurement area and stimulation point (b).

Mentions: The subjects were instructed to fast and refrain from smoking for at least 3 h prior to the experiment. They were asked to maintain natural breathing (10–15 times per min) and stay relaxed and awake throughout the experiment. Prior to starting the experiment, the subjects sat on a chair, with the right upper limb at rest in a vertical position and the left upper limb naturally extended and placed level with the heart. Two sets of NIRS probes were placed on the belly of the right trapezius muscle of the shoulder, with a 50-mm separation between probes (Fig. 1a &1b). We affixed the probe in the measurement area with surgical tape. First, the attachment edge of the probe was affixed. In addition, the probe's light source and detector were more strongly affixed to the measurement area using surgical tape extending from the right frontal area of the chest, over the shoulder and extending into the shoulder blade area. The measurement range was in the hemisphere of 1/2 of the distance between the light source and detector (20 mm) [16]. It is necessary to separate the measurement areas by 40 mm or more to prevent the near-infrared rays interfering when two NIRS are measured simultaneously. Therefore, we assumed a distance of 50 mm in consideration of this issue (Figure 1b). The distance between the paired light input and the detector was set at 40 mm, and the stimulation point was at the centre of the path between the light source and detector. We left a hole of a diameter of 8 mm in the probe between the light source and detector as shown in figure 1b. We covered all windows in the laboratory so that no extraneous light could enter. A fluorescent light was used in an area farthest away from the measurement point. Therefore, we were able to avoid the influence of other near infrared rays on the measurement value. We confirmed beforehand that the measurement of NIRS was not influenced by the creation of the hole. In this experiment, we determined the stimulation point anatomically in the center of the line, which tied up the acromion edge to the seventh cervical spine spinous process. This position is the same as GB21 (Jianjing) unified by WHO/WPRO [17]. The other probe located on the same muscle, 50 mm away from the stimulation region, was provided as the reference point (distant region). The subjects were instructed to report the perceived sensation at the end of AS to evaluate a subjective perception of the stimulation.


Local increase in trapezius muscle oxygenation during and after acupuncture.

Ohkubo M, Hamaoka T, Niwayama M, Murase N, Osada T, Kime R, Kurosawa Y, Sakamoto A, Katsumura T - Dyn Med (2009)

Measurement site and stimulation point (a) and measurement area and stimulation point (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662816&req=5

Figure 1: Measurement site and stimulation point (a) and measurement area and stimulation point (b).
Mentions: The subjects were instructed to fast and refrain from smoking for at least 3 h prior to the experiment. They were asked to maintain natural breathing (10–15 times per min) and stay relaxed and awake throughout the experiment. Prior to starting the experiment, the subjects sat on a chair, with the right upper limb at rest in a vertical position and the left upper limb naturally extended and placed level with the heart. Two sets of NIRS probes were placed on the belly of the right trapezius muscle of the shoulder, with a 50-mm separation between probes (Fig. 1a &1b). We affixed the probe in the measurement area with surgical tape. First, the attachment edge of the probe was affixed. In addition, the probe's light source and detector were more strongly affixed to the measurement area using surgical tape extending from the right frontal area of the chest, over the shoulder and extending into the shoulder blade area. The measurement range was in the hemisphere of 1/2 of the distance between the light source and detector (20 mm) [16]. It is necessary to separate the measurement areas by 40 mm or more to prevent the near-infrared rays interfering when two NIRS are measured simultaneously. Therefore, we assumed a distance of 50 mm in consideration of this issue (Figure 1b). The distance between the paired light input and the detector was set at 40 mm, and the stimulation point was at the centre of the path between the light source and detector. We left a hole of a diameter of 8 mm in the probe between the light source and detector as shown in figure 1b. We covered all windows in the laboratory so that no extraneous light could enter. A fluorescent light was used in an area farthest away from the measurement point. Therefore, we were able to avoid the influence of other near infrared rays on the measurement value. We confirmed beforehand that the measurement of NIRS was not influenced by the creation of the hole. In this experiment, we determined the stimulation point anatomically in the center of the line, which tied up the acromion edge to the seventh cervical spine spinous process. This position is the same as GB21 (Jianjing) unified by WHO/WPRO [17]. The other probe located on the same muscle, 50 mm away from the stimulation region, was provided as the reference point (distant region). The subjects were instructed to report the perceived sensation at the end of AS to evaluate a subjective perception of the stimulation.

Bottom Line: Two sets of near-infrared spectrometer (NIRS) probes, with 40-mm light-source detector spacing, were placed on the right trapezius muscle, with a 50-mm distance between the probes.Nine healthy acupuncture-experienced subjects were chosen as the experimental (AS) group, and 10 healthy acupuncture-experienced subjects were chosen for the control (no AS) group.In conclusion, oxygenation and blood volume increased, indicating elevated blood flow to the small vessels, not in the distant region used in this study, but in the stimulation region of the trapezius muscle during and after a 2-min AS.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Institute of Fitness and Sports in Kanoya, 1 Shiromizu, Kanoya, Kagoshima, Japan. ohkubo@kuretake.ac.jp

ABSTRACT

Purpose: This study aimed to compare the trapezius muscle blood volume and oxygenation in the stimulation region and in a distant region in the same muscle during acupuncture stimulation (AS). We hypothesized that AS provokes a localized increase in muscle blood volume and oxygenation in the stimulation region.

Methods: Two sets of near-infrared spectrometer (NIRS) probes, with 40-mm light-source detector spacing, were placed on the right trapezius muscle, with a 50-mm distance between the probes. Changes in muscle oxygenation (oxy-Hb) and blood volume (t-Hb) in stimulation and distant regions (50 mm away from the stimulation point) were measured using NIRS. Nine healthy acupuncture-experienced subjects were chosen as the experimental (AS) group, and 10 healthy acupuncture-experienced subjects were chosen for the control (no AS) group. Measurements began with a 3-min rest period, followed by "Jakutaku" (AS) for 2 min, and recovery after stimulation.

Results: There was a significant increase in oxy-Hb (60.7 muM at maximum) and t-Hb (48.1 muM at maximum) in the stimulation region compared to the distant region. In the stimulation region, a significant increase in oxy-Hb and t-Hb compared with the pre-stimulation level was first noted at 58.5 s and 13.5 s, respectively, after the onset of stimulation.

Conclusion: In conclusion, oxygenation and blood volume increased, indicating elevated blood flow to the small vessels, not in the distant region used in this study, but in the stimulation region of the trapezius muscle during and after a 2-min AS.

No MeSH data available.