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Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae).

Schetelig MF, Caceres C, Zacharopoulou A, Franz G, Wimmer EA - BMC Biol. (2009)

Bottom Line: These elements act differently in expression strength and their ability to drive lethal effector gene activation.Moreover, position effects strongly influence the efficiency of the system.Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Developmental Biology, Göttingen Center for Molecular Biosciences, Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology, Georg-August-University Göttingen, GZMB, Ernst-Caspari-Haus, Göttingen, Germany. marc.schetelig@ars.usda.gov

ABSTRACT

Background: The sterile insect technique (SIT) is an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae). Ionizing radiation used to generate reproductive sterility in the mass-reared populations before release leads to reduction of competitiveness.

Results: Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality. This system is dependent on newly isolated medfly promoter/enhancer elements of cellularization-specifically-expressed genes. These elements act differently in expression strength and their ability to drive lethal effector gene activation. Moreover, position effects strongly influence the efficiency of the system. Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality. This line was highly competitive to wildtype medfly in laboratory and field cage tests.

Conclusion: The high competitiveness of the transgenic lines and the achieved 100% embryonic lethality causing reproductive sterility without the need of irradiation can improve the efficacy of operational medfly SIT programs.

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Related in: MedlinePlus

tTA and hidAla5 expression depending on different integration sites. The expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hidAla5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.
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Figure 3: tTA and hidAla5 expression depending on different integration sites. The expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hidAla5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.

Mentions: Five driver constructs (sl1-tTA, sl2-tTA, 99-tTA, CG2186-tTA, and sryα 2-tTA) and the three effector constructs were used for germline transformation of medfly. For each construct we obtained transgenes of which we further analyzed a maximum of three independent lines (Figure 2 and Figure 3).


Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae).

Schetelig MF, Caceres C, Zacharopoulou A, Franz G, Wimmer EA - BMC Biol. (2009)

tTA and hidAla5 expression depending on different integration sites. The expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hidAla5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662800&req=5

Figure 3: tTA and hidAla5 expression depending on different integration sites. The expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hidAla5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.
Mentions: Five driver constructs (sl1-tTA, sl2-tTA, 99-tTA, CG2186-tTA, and sryα 2-tTA) and the three effector constructs were used for germline transformation of medfly. For each construct we obtained transgenes of which we further analyzed a maximum of three independent lines (Figure 2 and Figure 3).

Bottom Line: These elements act differently in expression strength and their ability to drive lethal effector gene activation.Moreover, position effects strongly influence the efficiency of the system.Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Developmental Biology, Göttingen Center for Molecular Biosciences, Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology, Georg-August-University Göttingen, GZMB, Ernst-Caspari-Haus, Göttingen, Germany. marc.schetelig@ars.usda.gov

ABSTRACT

Background: The sterile insect technique (SIT) is an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae). Ionizing radiation used to generate reproductive sterility in the mass-reared populations before release leads to reduction of competitiveness.

Results: Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality. This system is dependent on newly isolated medfly promoter/enhancer elements of cellularization-specifically-expressed genes. These elements act differently in expression strength and their ability to drive lethal effector gene activation. Moreover, position effects strongly influence the efficiency of the system. Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality. This line was highly competitive to wildtype medfly in laboratory and field cage tests.

Conclusion: The high competitiveness of the transgenic lines and the achieved 100% embryonic lethality causing reproductive sterility without the need of irradiation can improve the efficacy of operational medfly SIT programs.

Show MeSH
Related in: MedlinePlus